Neurobiology of Steroids, Volume 22 (Methods in Neurosciences) (Vol 22)

Steroid hormones are unique compounds in that they are active at the interface of peripheral endocrine events and neural mechanisms. Thus their effects present an important peripheral signaling system to alter brain function. This volume presents state-of-the-art and classical techniques for the study of steroid hormones and their receptors and their effects and actions. Key Features Comprehensive protocols included for the study of * Steroid kinetics and metabolism * Steroid receptors * Molecular and cellular effects of steroids * Steroid effects on integrated systems Content: Methods in Neurosciences Page ii Front Matter Page iii Copyright page Page iv Contributors to Volume 22 Pages ix-xiii Preface Page xv E. RONALD DE KLOET, WIN SUTANTO Methods in Neurosciences Pages xvii-xviii 1 - Steroid Hormone Transport through Blood–Brain Barrier: Methods and Concepts Pages 3-22 William M. Pardridge 2 - Enhanced Delivery of Steroids to the Brain Using a Redox-Based Chemical Delivery System Pages 23-35 James W. Simpkins, Nicholas Bodor 3 - Neurosteroids: Biosynthesis and Function Pages 36-50 Paul Robel, Etienne-Emile Baulieu 4 - Cytochrome P450 Enzymes in Brain Pages 51-66 Margaret Warner, Adrian Wyss, Shigetaka Yoshida, Jan-Åke Gustafsson 5 - Methods for Estimating 11β-Hydroxysteroid Dehydrogenase Activity Pages 67-76 Zygmunt Krozowski 6 - Steroid Hormone Binding to Intracellular Receptors: In Vitro and in Vivo Studies Pages 79-95 Aernout D. van Haarst, Thomas F. Szuran 7 - Steroid Hormone Binding to Membrane Receptors Pages 96-115 Miles Orchinik, Thomas F. Murray 8 - In Vitro Autoradiography for Steroid Receptors Pages 116-142 Neil J. MacLusky, He Yuan, Debbie Bowlby, Richard B. Hochberg, Theodore J. Brown 9 - Immunocytochemical Studies on Glucocorticoid Receptor Pages 143-161 Antonio Cintra, Gunnar Akner, Rafael Coveñas, Mercedes de León, Ann-Charlotte Wikström, Luigi F. Agnati, Jan-Åke Gustafsson, Kjell Fuxe 10 - Confocal Scanning Laser Microscopy of Steroid Receptors in Brain Pages 162-174 Bas van Steensel, Erica P. van Binnendijk, Roel van Driel 11 - Ultrastructural Aspects of Steroid Receptor Localization: Immunocytochemical Perspective Pages 175-188 Zsolt Liposits, Imre Kalló 12 - Hybridization Studies of Adrenocorticosteroid Receptors in the Central Nervous System Pages 189-210 James P. Herman, Seung P. Kwak, Stanley J. Watson 13 - Detection and Characterization of Epalon Receptors: Novel Recognition Sites for Neuroactive Steroids That Modulate the GABA A Receptor Complex Pages 211-225 Linda D. McCauley, Kelvin W. Gee 14 - Mutation Analysis of Steroid Hormone Receptors Pages 226-241 Michael Karl, Heinrich M. Schulte, George P. Chrousos 15 - Protein–DNA-Binding Assay for Analysis of Steroid-Sensitive Neurons in Mammalian Brain Pages 245-264 Yuan-shan Zhu, Donald W. Pfaff 16 - Gene Transfection Studies Using Recombinant Steroid Receptors Pages 265-276 Klaus Damm 17 - Regulation of Neuropeptide Genes: Determination of Responsiveness to Steroids and Identification of Receptors in Brain Nuclei Pages 277-295 Joke J. Cox, Sofia Lopes da Silva, Wiljan Hendriks, J. Peter H. Burbach 18 - Cloning of Steroid-Responsive mRNAs by Differential Hybridization Pages 296-313 Nancy R. Nichols, Jeffrey N. Masters, Caleb E. Finch 19 - Molecular Correlates of Corticosterone Action in Hippocampal Subregions Pages 314-329 Suresh M. Nair, James H. Eberwine 20 - Glucocorticoid-Determined Protein Synthesis Pages 330-341 Linda A. Dokas 21 - Use of Antisense Oligodeoxynucleotides to Block Gene Expression in Central Nervous System Pages 342-356 Margaret M. McCarthy 22 - Steroid Regulation of Neuronotrophic Activity: Primary Microcultures of Midbrain Raphe and Hippocampus Pages 359-371 Efrain C. Azmitia, Xiao Ping Hou 23 - Central Glucocorticoid Receptors and Neuronal Plasticity Pages 372-382 Kjell Fuxe, Antonio Cintra, Gerson Chadi, Jan-Åke Gustafsson, Luigi F. Agnati 24 - Steroid Action on Neuronal Structure Pages 383-402 Catherine S. Woolley, Elizabeth Gould 25 - Electron Microscopic Double and Triple Labeling Immunocytochemistry in Elucidation of Synaptological Interactions between Ovarian Steroid-Sensitive Neurons and Circuits Pages 403-416,416a,417-434 Csaba Leranth, Frederick Naftolin, Marya Shanabrough, Tamas L. Horvath 26 - Gene-Mediated Steroid Control of Neuronal Activity Pages 435-445 Marian Joëls 27 - Electrophysiological Studies of Neurosteroid Modulation of γ-Aminobutyric Acid Type A Receptor Pages 446-467 C. Hill-Venning, D. Belelli, J.A. Peters, J.J. Lambert 28 - In Vitro Approaches to Studying Glucocorticoid Effects on Gene Expression in Neurons and Glia Pages 468-480 Martha Churchill Bohn 29 - Behavioral Approaches to Study Function of Corticosteroids in Brain Pages 483-495 Melly Silvana Oitzl 30 - Adrenocorticosteroids and Cardiovascular Regulation: Methods for Surgery and Blood Pressure Measurements Pages 496-509 Elise P. Gómez Sánchez 31 - Steroids and Central Regulation of Immune Response Pages 510-522 Bernd Schöbitz 32 - Steroid Hormone Effects on Brain: Novel Insights Connecting Cellular and Molecular Features of Brain Cells to Behavior Pages 525-542 Bruce S. McEwen Index Pages 543-552 Steroid hormones are unique compounds in that they are active at the interface of peripheral endocrine events and neural mechanisms. Thus their effects present an important peripheral signaling system to alter brain function. This volume presents state-of-the-art and classical techniques for the study of steroid hormones and their receptors and their effects and actions.

Key Features
Comprehensive protocols included for the study of
* Steroid kinetics and metabolism
* Steroid receptors
* Molecular and cellular effects of steroids
* Steroid effects on integrated systems This volume presents state-of-the-art and classical techniques for the study of steroid hormones. Protocols are included for the study of: steroid kinetics and metabolism; steroid receptors; molecular and cellular effects of steroids; and steroid effects on integrated systems.