Neoplastic Transformation in Human Cell Culture: Mechanisms of Carcinogenesis (Experimental Biology and Medicine, 25)

The role of carcinogenic agents in the deveolopment of human cancers is now being defined using a variety of human cells as experi­ mental model systems. A workshop on "neoplastic transformation in human cell systems in vitro: mechanisms of carcinogenesis" was held at the Georgetown University Medical Center, Washington, DC, on April 25-26, 1991. The aims of the workshop were to present the state-of-the­ art in the transformation of human cells in culture, as well as to provide insight into the molecular and cellular changes involved in the conver­ sion of normal cells to a neoplastic state of growth. The following topics were closely related to the theme of the workshops: 1. Derivation of in vitro model systems (epithelial, fibroblastic, and hematopoietic). 2. Factors modulating cellular transformation. 3. Usefulness of defined in vitro model systems for viral, chemical, and radiation carcinogenesis. 4. Multistep nature of human cell carcinogenesis. 5. Role of activated and suppressor oncogenes in neoplastic trans­ formation. The workshop was organized by J. S. Rhim and A. Dritschilo (cochairmen), G. Jay, J. little, M. McCormick, R Tennant, and R R Weischelbaum. There were 32 speakers, 30 poster presentations, and about 190 participants. Front Matter....Pages i-xxxi Front Matter....Pages 1-1 Comparison of Human Versus Rodent Cell Transformation: Importance of Cell Aging....Pages 3-13 Deficient DNA Repair, an Early Step in Neoplastic Transformation of Human Cells in Culture....Pages 15-23 Use of Immortalized Human Keratinocytes for the Study of Squamous Differentiation and Mutagenesis....Pages 25-34 Studies of Mutagen-Activated Genes which Confer Anchorage-Independence: the c-sis Gene as a Model....Pages 35-46 Cytoskeletal Changes in Human Transformed Cells: Studies on HOS Cells....Pages 47-56 Polyamine Metabolism in Human Epidermal Keratinocytes Transformed with AD12-SV40, HPV16-DNA and K- ras Oncogene....Pages 57-64 Front Matter....Pages 65-65 Transformation of Human Diploid Fibroblasts by Radiation and Oncogenes....Pages 67-79 Ionizing Radiation-Mediated Protein Kinase C Activation and Gene Expression....Pages 81-92 Detection of Transforming Genes from Radiation Transformed Human Epidermal Keratinocytes by a Tumorigenicity Assay....Pages 93-101 Neoplastic Transformation of Human Epithelial Cells by Ionizing Radiation....Pages 103-111 Effects of Ionizing Radiation on Human Papillomavirus Immortalized Human Bronchial Epithelial Cells....Pages 113-117 Biochemical Purification of a CSF-1 like Molecule Released during Malignant Transformation of IL-3 Dependent Hematopoietic Progenitor cell lines Cocultivated with Gamma Irradiated Clonal Marrow Stromal cell lines....Pages 119-123 An Inherited P53 Point Mutation in a Cancer Prone Family with Li-Fraumeni Syndrome....Pages 125-134 p53, a Direct Target of Mutational Activation by Chemical Carcinogens?....Pages 135-139 Front Matter....Pages 141-141 The HIV tat Gene Induces Epidermal Hyperplasia in vivo and Transforms Keratinocytes in vitro ....Pages 143-152 Immortalization and Tumorigenic Transformation of Normal Human Cervical Epithelial Cells Transfected with Human Papillomavirus DNAs....Pages 153-161 Using the Papillomavirus E6/E7 Genes to Generate Well-Differentiated Epithelial Cell Lines....Pages 163-167 Tumor Progression in Breast Cancer....Pages 169-178 Growth Regulation of HPV-Positive Keratinocytes by TGF- β l....Pages 179-189 Cell Growth Transformation by Epstein Barr Virus....Pages 191-201 Front Matter....Pages 141-141 Isolation and Characterization of a Transformation-Associated Gene from Human Nasopharyngeal Carcinoma Cells....Pages 203-212 Molecular Pathogenesis of Lung Cancer....Pages 213-221 Molecular Control of Expression of Plasticity of Tumorigenic/Metastatic Phenotypes....Pages 223-232 Front Matter....Pages 233-233 Altered Regulation of Growth and Differentiation at Different Stages of Transformation of Human Skin Keratinocytes....Pages 235-250 Neoplastic Transformation and Suppression of Transformation of Human Bronchial Epithelial Cells in vitro ....Pages 251-258 An in vitro Human Mammary Epithelial Model System for Studies of Differentiation and Carcinogenesis....Pages 259-267 Transformation in vitro of Human Uroepithelial Cells....Pages 269-280 Multiple Steps in the in vitro Immortalisation and Neoplastic Conversion of Human Colonic Epithelial Cells....Pages 281-290 A Human Renal Epithelial Multistep Model of in vitro Carcinogenesis....Pages 291-296 Immortalization and Oncogene Transformation of Human Esophageal Epithelial Cells....Pages 297-305 A Non-Tumorigenic Human Liver Epithelial Cell Culture Model for Chemical and Biological Carcinogenesis Investigations....Pages 307-321 Establishment and Characterization of SV40 T-Antigen Immortalized Human Liver Cells....Pages 323-332 Transformation of Human Tracheal Gland Epithelial Cells in vitro ....Pages 333-341 Stable Expression of SV40 Large T-Antigen Gene in Primary Human Schwann Cells....Pages 343-346 Malignant Transformation of Human Fibroblasts in vitro ....Pages 347-357 Mitogen-independence and Autocrine Growth Factor Secretion Displayed by Human Mesothelioma Cells and Oncogene-Transfected Mesothelial Cells....Pages 359-366 Structure and Growth Regulation in Normal, Transformed and Malignant Human Endometrial Cell Cultures....Pages 367-376 Construction of a Unidirectional cDNA Library from a Radioresistant Laryngeal Squamous Cell Carcinoma Cell Line in an Epstein Barr Virus Shuttle Vector....Pages 377-386 Back Matter....Pages 387-396 The role of carcinogenic agents in the deveolopment of human cancers is now being defined using a variety of human cells as experiƯ mental model systems. A workshop on "neoplastic transformation in human cell systems in vitro: mechanisms of carcinogenesis" was held at the Georgetown University Medical Center, Washington, DC, on April 25-26, 1991. The aims of the workshop were to present the state-of-theƯ art in the transformation of human cells in culture, as well as to provide insight into the molecular and cellular changes involved in the converƯ sion of normal cells to a neoplastic state of growth. The following topics were closely related to the theme of the workshops: 1. Derivation of in vitro model systems (epithelial, fibroblastic, and hematopoietic). 2. Factors modulating cellular transformation. 3. Usefulness of defined in vitro model systems for viral, chemical, and radiation carcinogenesis. 4. Multistep nature of human cell carcinogenesis. 5. Role of activated and suppressor oncogenes in neoplastic transƯ formation. The workshop was organized by J.S. Rhim and A. Dritschilo (cochairmen), G. Jay, J. little, M. McCormick, R Tennant, and R R Weischelbaum. There were 32 speakers, 30 poster presentations, and about 190 participants