Natural Biomarkers for Cellular Metabolism: Biology, Techniques, and Applications (Series in Cellular and Clinical Imaging)

__From the Lab to Clinical Settings—Advances in__ __Quantitative, Noninvasive Optical Diagnostics__ Noninvasive fluorescence imaging techniques, novel fluorescent labels, and natural biomarkers are revolutionizing our knowledge of cellular processes, signaling and metabolic pathways, the underlying mechanisms for health problems, and the identification of new therapeutic targets for drug discoveries. **Natural Biomarkers for Cellular Metabolism: Biology, Techniques, and Applications** delves into the current state of knowledge on intrinsic fluorescent biomarkers and highlights recent developments in using these biomarkers for the metabolic mapping and clinical diagnosis of healthy and diseased cells and tissues. __Autofluorescent Biomarkers for Biomedical Diagnostics__ The book’s first section introduces the fundamentals of cellular energy metabolism as well as natural biomarkers within the context of their biological functions. The second section outlines the theoretical and technical background of quantitative, noninvasive, autofluorescence microscopy and spectroscopy methods, including experimental design, calibration, pitfalls, and remedies of data acquisition and analysis. The last two sections highlight advances in biomedical and biochemical applications, such as monitoring stem cell differentiation in engineered tissues and diagnosing cancer and ophthalmic diseases quantitatively and noninvasively. __Tailored to Interdisciplinary Researchers__ Covering cell biology, imaging techniques, and clinical diagnostics, this book provides readers with a complete guide to studying cellular/tissue metabolism under healthy, diseased, and environment-induced stress conditions using natural biomarkers. The book is designed for graduate and advanced undergraduate students, biophysics instructors, medical researchers, and those in pharmaceutical R&D. Biochemical, Biological, And Biophysical Background -- Mitochondria And Energy Metabolism : Networks, Mechanisms And Control / Ilmo Hassinen -- Intracellular Autofluorescence Species : Structure, Spectroscopy, And Photophysics / Nobuhiro Ohta And Takakazu Nakabayashi -- Autofluorescence Imaging Techniques : Fundamentals And Applications -- One-photon Autofluorescence Microscopy / Narasimhan Rajaram And Nirmala Ramanujam -- Autofluorescence Lifetime Imaging / Michael G. Nichols, Kristina Ward, Lyandysha V. Zholudeva, Heather Jensen Smith And Richard Hallworth -- Polarization Imaging Of Cellular Autofluorescence / Harshad D. Vishwasrao, Qianru Yu, Kuravi Hwawasam, And Ahmed A. Heikal -- Real-time In Vivo Monitoring Of Cellular Energy Metabolism / Avraham Mayevsky And Efrat Barbiro-michaely -- Tryptophan As An Alternative Biomarker For Cellular Energy Metabolism / Vinod Jyothikumar, Yuansheng Sun, And Ammasi Periasamy -- Alternative Approaches To Optical Sensing Of The Redox State / Yi Yang -- Natural Biomarkers For Biochemical And Biological Studies -- Spatiotemporal Detection Of Nadh-linked Enzyme Activities / V. Krishnan Ramanujan -- Nad(p)h And Fad As Biomarkers For Programmed Cell Death / Hsing-wen Wang -- Monitoring Stem Cell Differentiation In Engineered Tissues / Kyle P. Quinn And Irene Georgakoudi -- Autofluorescence As A Diagnostic Tool In Medicine And Health -- Autofluorescence-assisted Examination Of Cardiovascular System Physiology And Pathology / Alzbeta Chorvatova -- Autofluorescence Perspectives Of Cancer Diagnostics / Lin Z. Li And Nannan Sun -- Dynamic Imaging Of Intracellular Coenzyme Autofluorescence In Intact Pancreatic Islets / Alan K. Lam And Jonathan V. Rocheleau -- Autofluorescence Diagnostics Of Ophthalmic Diseases / Dietrich Schweitzer -- Pathogen Effects On Energy Metabolism In Host Cells / Márta Szaszák And Jan Rupp. Edited By Vladimir V. Ghukasyan, Ahmed A. Heikal. Includes Bibliographical References And Index.