Multiphoton Microscopy

This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In __Neuromethods__ series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Comprehensive and thorough, __Multiphoton Microscopy__ is a valuable resource for both expert and novice researchers interested in expanding their knowledge and research in this rapidly developing field. Front Matter ....Pages i-xii Building a Two-Photon Microscope Is Easy (Spencer LaVere Smith)....Pages 1-16 Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas (Qiang Chen, Wei Wei)....Pages 17-31 Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration (Alexandra Tran-Van-Minh, Nelson Rebola, Andreas Hoehne, David A. DiGregorio)....Pages 33-64 Two-Photon Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines (Ivar S. Stein, Travis C. Hill, Won Chan Oh, Laxmi K. Parajuli, Karen Zito)....Pages 65-85 Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release Using iGluSnFR in the Mouse Visual System (Bart G. Borghuis)....Pages 87-109 Imaging Neuronal Signal Transduction Using Multiphoton FRET-FLIM (Paul R. Evans, Long Yan, Ryohei Yasuda)....Pages 111-130 Combining Multiphoton Excitation Microscopy with Fast Microiontophoresis to Investigate Neuronal Signaling (Espen Hartveit, Margaret Lin Veruki)....Pages 131-159 Multiphoton Excitation Microscopy for the Reconstruction and Analysis of Single Neuron Morphology (Espen Hartveit, Bas-Jan Zandt, Margaret Lin Veruki)....Pages 161-194 Serial Multiphoton Tomography and Analysis of Volumetric Images of the Mouse Brain (Denise M. O. Ramirez, Apoorva D. Ajay, Mark P. Goldberg, Julian P. Meeks)....Pages 195-224 Studying a Light Sensor with Light: Multiphoton Imaging in the Retina (Thomas Euler, Katrin Franke, Tom Baden)....Pages 225-250 Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach (Rune Enger, Rolf Sprengel, Erlend A. Nagelhus, Wannan Tang)....Pages 251-277 Two-Photon Imaging of Dendritic Calcium Dynamics In Vivo (Lucy M. Palmer)....Pages 279-295 Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy (Christopher J. Roome, Bernd Kuhn)....Pages 297-334 High-Speed, Random-Access Multiphoton Microscopy for Monitoring Synaptic and Neuronal Activity in 3D in Behaving Animals (Mate Marosi, Gergely Szalay, Gergely Katona, Balázs Rózsa)....Pages 335-362 Back Matter ....Pages 363-364 "This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and thorough, Multiphoton Microscopy is a valuable resource for both expert and novice researchers interested in expanding their knowledge and research in this rapidly developing field." -- prové de l'editor