Molecular Biotechnology : Principles and Applications of Recombinant DNA (4th Edition)

Acclaimed by students and instructors, Molecular Biotechnology: Principles and Applications of Recombinant DNA is now in its fourth edition, bringing it thoroughly up to date with the latest findings and the latest industrial, agricultural, pharmaceutical, and biomedical applications. At the same time, the text maintains all the hallmarks that have made it a bestseller. These include its straightforward, jargon-free writing style and its extensive use of figures that help students make sense of complex biological systems and processes. These features not only enable students to grasp core concepts, but also create the foundation needed to support their own research and development work using recombinant DNA technology. This fourth edition features greatly expanded coverage of the latest innovations in DNA sequencing techniques, therapeutics, vaccines, transgenic plants, and transgenic animals. Moreover, readers will find nearly 240 new figures to help them grasp all the latest concepts and applications. With its broad range of topics, Molecular Biotechnology is adaptable to different upper-level undergraduate and graduate courses emphasizing particular aspects of modern biotechnology. For example, instructors can easily tailor the content to courses focusing on the fundamentals of biotechnology as well as courses dedicated to medical, agricultural, environmental, or industrial applications. Key Features * New edition presents 645 figures and 113 tables throughout the text, hundreds of which illustrate complex systems and processes * Chapter summaries highlighting key points * References to the literature in each chapter facilitating detailed investigations of all topics covered in the text * End-of-chapter review questions enabling students to assess their knowledge * Updated examples illustrating the latest concepts and applications Cover Page 1 Title Page 4 ISBN 9781555814984 5 Contents 8 Preface 14 Preface to the First Edition 16 PART I: FUNDAMENTALS OF MOLECULAR BIOTECHNOLOGY 20 Chapter 1 The Development of Molecular Biotechnology 22 The Emergence of Molecular Biotechnology 22 Recombinant DNA Technology 24 Commercialization of Molecular Biotechnology 25 Concerns and Consequences 29 SUMMARY 31 REFERENCES 32 REVIEW QUESTIONS 32 Chapter 2 DNA, RNA, And Protein Synthesis 33 Structure of DNa 33 DNA Replication 37 Decoding Genetic Information: RNA and Protein 39 Translation 45 Regulation of mRNA Transcription in Bacteria 52 Regulation of mRNA transcription in Eukaryotes 56 Protein Secretion Pathways 59 SUMMARY 64 REFERENCES 65 REVIEW QUESTIONS 65 Chapter 3 Recombinant DNA technology 66 Restriction Endonucleases 68 Plasmid Cloning Vectors 76 Creating and Screening a Library 87 Cloning DNA Sequences that Encode Eukaryotic Proteins 99 Vectors for Cloning Large Pieces of DNA 105 Genetic Transformation of Prokaryotes 111 SUMMARY 114 REFERENCES 115 REVIEW QUESTIONS 116 Chapter 4 Chemical Synthesis, Amplification, and Sequencing of DNA 117 Chemical Synthesis of DNA 117 Polymerase Chain Reaction 127 DNA-Sequencing Techniques 136 Large-Scale DNA Sequencing 152 SUMMARY 161 REFERENCES 162 REVIEW QUESTIONS 164 Chapter 5 Bioinformatics, Genomics, and Proteomics 165 Molecular Databases 165 Metagenomics 167 Functional Genomics 173 Proteomics 183 SUMMARY 208 REFERENCES 209 REVIEW QUESTIONS 212 Chapter 6 Manipulation of Gene Expression in Prokaryotes 214 Gene Expression From Strong and Regulatable Promoters 215 Fusion Proteins 224 Translation Expression Vectors 231 Increasing Protein Stability 234 Overcoming Oxygen Limitation 239 Limiting Biofilm Formation 240 DNA Integration into the Host Chromosome 241 Increasing Secretion 247 Metabolic Load 252 SUMMARY 254 REFERENCE 255 REVIEW QUESTIONS 257 Chapter 7 Heterologous Protein Production in Eukaryotic Cells 259 Posttranslational Modification of Eukaryotic Proteins 259 General Features of Eukaryotic Expression Systems 261 Fungus-Based Expression Systems 263 Baculovirus–Insect Cell Expression Systems 280 Mammalian Cell Expression Systems 290 SUMMARY 305 REFERENCES 306 REVIEW QUESTIONS 307 Chapter 8 Directed Mutagenesis and Protein Engineering 309 Directed Mutagenesis Procedures 310 Protein Engineering 324 SUMMARY 346 REFERENCES 346 REVIEW QUESTIONS 347 PART II: MOLECULAR BIOTECHNOLOGY OF MICROBIAL SYSTEMS 350 Chapter 9 Molecular Diagnostics 352 Immunological Diagnostic Procedures 353 Monoclonal Antibodies 356 Biofluorescent and Bioluminescent Systems 360 Nucleic Acid Diagnostic Systems 364 Molecular Diagnosis of Genetic Disease 384 SUMMARY 394 REFERENCES 395 REVIEW QUESTIONS 396 Chapter 10 Protein Therapeutics 398 Pharmaceuticals 399 Enzymes 408 Lactic Acid Bacteria 414 Monoclonal Antibodies 418 Recombinant Antibodies 422 SUMMARY 441 REFERENCES 441 REVIEW QUESTIONS 443 Chapter 11 Nucleic Acids as Therapeutic Agents 445 Antisense RNA 445 Ribozymes 453 Chimeric RNA–DNA Molecules 456 Aptamers 456 Interfering RNAs 459 Antibody Genes 462 Nucleic Acid Delivery 463 SUMMARY 475 REFERENCES 475 REVIEW QUESTIONS 477 Chapter 12 Vaccines 478 Subunit Vaccines 482 Peptide Vaccines 488 Genetic Immunization: DNA Vaccines 491 Attenuated Vaccines 500 Vector Vaccines 505 SUMMARY 516 REFERENCES 516 REVIEW QUESTIONS 518 Chapter 13 Synthesis of Commercial Products by Recombinant Microorganisms 520 Restriction Endonucleases 520 Lipase 524 Small Biological Molecules 525 Antibiotics 540 Biopolymers 554 SUMMARY 566 REFERENCES 566 REVIEW QUESTIONS 568 Chapter 14 Bioremediation and Biomass Utilization 570 Microbial Degradation of Xenobiotics 570 Genetic Engineering of Biodegradative Pathways 575 Utilization of Starch and Sugars 588 Utilization of Cellulose 599 Hydrogen Production 614 SUMMARY 615 REFERENCES 615 REVIEW QUESTIONS 617 Chapter 15 Plant Growth-promoting Bacteria 618 Growth Promotion by free-Living Bacteria 619 Biocontrol of Pathogens 627 Nitrogen Fixation 638 Hydrogenase 649 Nodulation 654 Phytoremediation 660 SUMMARY 667 REFERENCES 668 REVIEW QUESTIONS 670 Chapter 16 Microbial Insecticides 671 Insecticidal Toxin of B. Thuringiensis 672 Engineering of B. Thuringiensis Toxin Genes 679 Baculoviruses as Biocontrol Agents 696 SUMMARY 700 REFERENCES 701 REVIEW QUESTIONS 703 Chapter 17 Large-Scale Production of Proteins From Recombinant Microorganisms 704 Principles of Microbial Growth 706 Maximizing the Efficiency of the Fermentation Process 711 Bioreactors 720 Typical Large-Scale Fermentation Systems 724 Harvesting Microbial Cells 730 Disrupting Microbial Cells 733 Downstream Processing 736 Large-Scale Production of Plasmid DNA 738 SUMMARY 739 REFERENCES 739 REVIEW QUESTIONS 741 PART III: MOLECULAR BIOTECHNOLOGY OF EUKARYOTIC SYSTEMS 742 Chapter 18 Genetic Engineering of Plants: Methodology 744 Plant Transformation with the Ti Plasmid of A. Tumefaciens 745 Ti Plasmid-Derived Vector Systems 749 Physical Methods of Transferring Genes to Plants 754 Chloroplast Engineering 757 Use of Reporter Genes in Transformed Plant Cells 760 Manipulation of Gene Expression in Plants 762 Production of Marker-free Transgenic Plants 769 SUMMARY 774 REFERENCES 775 REVIEW QUESTIONS 776 Chapter 19 Engineering Plants to Overcome Biotic and Abiotic Stress 778 Insect Resistance 778 Virus Resistance 792 Herbicide Resistance 801 Fungus and Bacterium Resistance 806 Oxidative Stress 811 Salt and Drought Stress 812 Fruit Ripening and Flower Wilting 815 SUMMARY 818 REFERENCES 819 REVIEW QUESTIONS 821 Chapter 20 Engineering Plant Quality and Proteins 822 Modification of Plant Nutritional Content 822 Modification of Food Plant Taste and Appearance 834 Genetic Manipulation of Flower Pigmentation 840 Plants as Bioreactors 844 Edible Vaccines 849 Plant Yield 851 Phytoremediation 857 SUMMARY 860 REFERENCES 860 REVIEW QUESTIONS 862 Chapter 21 Transgenic Animals 864 Transgenic Mice: Methodology 866 Transgenic Mice: Applications 882 Cloning Livestock by Nuclear Transfer 890 Transgenic Livestock 892 Transgenic Poultry 904 Transgenic Fish 905 SUMMARY 909 REFERENCES 909 REVIEW QUESTIONS 912 PART IV: MOLECULAR BIOTECHNOLOGY AND SOCIETY 914 Chapter 22 Regulating the use of Biotechnology 916 Regulating Recombinant DNA Technology 917 Deliberate Release of Genetically Modified Microorganisms 919 Regulating Food and Food Ingredients 922 Patenting Biotechnology 930 SUMMARY 939 REFERENCES 939 REVIEW QUESTIONS 940 Chapter 23 Societal Issues in Biotechnology 942 Concerns About the Safety of Consuming Genetically Modified Foods 942 Concerns About the Impact of Genetically Modified Organisms on the Environment 951 Economic Issues 954 SUMMARY 956 REFERENCES 957 REVIEW QUESTIONS 958 Amino Acids of Proteins and Their Designations 960 Glossary 962 A 962 B 964 C 965 D 968 E 970 F 971 G 972 H 974 I 975 J 976 K 976 L 976 M 977 N 979 O 979 P 980 Q 983 R 983 S 985 T 988 U 990 V 990 W 990 X 990 Y 991 Z 991 Index 992 A 992 B 994 C 995 D 998 E 1000 F 1001 G 1002 H 1004 I 1004 J 1005 K 1005 L 1005 M 1006 N 1008 O 1009 P 1009 Q 1013 R 1013 S 1014 T 1017 U 1018 V 1018 W 1019 X 1019 Y 1019 Z 1019 Back Page 1020 ISBN,9781555814984 Acclaimed by students and instructors, "Molecular Biotechnology: Principles and Applications of Recombinant DNA" is now in its fourth edition, bringing it thoroughly up to date with the latest findings and the latest industrial, agricultural, pharmaceutical, and biomedical applications. At the same time, the text maintains all the hallmarks that have made it a bestseller. These include its straightforward, jargon-free writing style and its extensive use of figures that help students make sense of complex biological systems and processes. These features not only enable students to grasp core concepts, but also create the foundation needed to support their own research and development work using recombinant DNA technology. This fourth edition features greatly expanded coverage of the latest innovations in DNA sequencing techniques, therapeutics, vaccines, transgenic plants, and transgenic animals. Moreover, readers will find nearly 240 new figures to help them grasp all the latest concepts and applications. With its broad range of topics, Molecular Biotechnology is adaptable to different upper-level undergraduate and graduate courses emphasizing particular aspects of modern biotechnology. For example, instructors can easily tailor the content to courses focusing on the fundamentals of biotechnology as well as courses dedicated to medical, agricultural, environmental, or industrial applications. New edition presents 645 figures and 113 tables throughout the text, hundreds of which illustrate complex systems and processes. It includes chapter summaries highlighting key points. It contains references to the literature in each chapter facilitating detailed investigations of all topics covered in the text. It features end-of-chapter review questions enabling students to assess their knowledge. There are updated examples illustrating the latest concepts and applications Completely revised and updated, the second edition of the best-selling Molecular Biotechnology: Principles and Applications of Recombinant DNA covers both the underlying scientific principles and the wide-ranging industrial, agricultural, pharmaceutical, and biomedical applications of recombinant DNA technology. Ideally suited as a text, this book is also an excellent reference for health professionals, scientists, engineers, or attorneys interested in biotechnology.

The book contains predominantly color illustrations, with some black-and-white illustrations.

Covers both the underlying scientific principles and the wide-ranging industrial, agricultural, pharmaceutical, and biomedical applications of recombinant DNA technology.

"Completely revised and updated, the second edition of the best-selling Molecular Biotechnology: Principles and Applications of Recombinant DNA covers both the underlying scientific principles and the wide-ranging industrial, agricultural, pharmaceutical, and biomedical applications of recombinant DNA technology." "Ideally suited as a text, this book is also an excellent reference for health professionals, scientists, engineers, or attorneys interested in biotechnology."--BOOK JACKET. A unique, adaptable textbook for upper-level undergraduate and graduate courses emphasizing particular aspects of modern biotechnology. • Allows instructors to easily tailor the content to courses focusing on the fundamentals of biotechnology as well as courses dedicated to medical, agricultural, environmental, or industrial applications.