تکنیک‌های زیست‌شناسی مولکولی: یک دوره آزمایشگاهی فشرده

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features\* Provides well-tested experimental protocols for each technique\* Lists the reagents and preparation of each experiment separately\* Contains a complete schedule of experiments and the preparation required\* Includes study questions at the end of each chapter This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.

Key Features
* Provides well-tested experimental protocols for each technique
* Lists the reagents and preparation of each experiment separately
* Contains a complete schedule of experiments and the preparation required
* Includes study questions at the end of each chapter The purpose of CsCl-ethidium bromide density gradient centrifugation is to separate supercoiled (covalently closed circular) plasmid DNA from other components present in lysates of bacterial cells: linear DNA (chromosomal fragments), nicked plasmid DNA (which lacks supercoils), RNA, proteins, and carbohydrates. Anyone who need very nice information should read it.The book provide quite day to day information to fit lab routine.It Also explain some impresive insight on future technics. Wiht out doubt I recomended.I gave it 5 star because I use it everyday at work. Lab.Gen e Molecular Biology. InCor. Brazil