Molecular Biology of Neuroreceptors and Ion Channels (Nato ASI Subseries H:, 32)

This workshop was the second of this series held on the island of santorini in the Cycladic Sea. The first one ("Mechanism of Action of the Nicotinic Acetylcholine Receptor", NATO ASI Se℗Ư ries H, vol. 10) took place in May 1986 and focused on what was at the time the best studied of all neuroreceptors. This second one, held only two years later, demonstrates the im℗Ư mense progress achieved since then in the field of neurorecep℗Ư tors and ion channels. Molecular cloning techniques have now made available the primary structures of a whole array of ion channel proteins, and this in turn has shed light on some gen℗Ư eral principles of the structure-function relationships of these central elements of intercellular communication. The purpose of this workshop was to explore the common ele℗Ư ments in gene and protein structure of already cloned ion channel proteins, and to assess the status of other cloning projects in progress. It explicitly focused on very recently published and unpublished results. All participants kept to these goals thereby demonstrating the very value of such work℗Ư shops for the progress of science Front Matter....Pages I-XXI Common Structural Principles of Ion Channel Proteins....Pages 1-11 Brain and Muscle Nicotinic Acetylcholine Receptors: A Gene Family....Pages 13-30 The Gene Family Encoding Neuronal Acetylcholine Receptors: Structure of the Avian Alpha2 and Alpha3 Genes....Pages 31-36 Structure of Muscle and Neuronal Nicotinic Acetylcholine Receptors....Pages 37-53 Nicotinic Acetylcholine Receptors in the Nervous System of Insects....Pages 55-68 Ligand-Gated Ion Channels of Drosophila ....Pages 69-81 The Structure and Expression of the GABA A Receptor as Deduced by Molecular Genetic Studies....Pages 83-99 GABA A receptor: subunit expression in Xenopus oocytes and localization of receptor mRNAs in rat brain....Pages 101-117 Molecular Heterogeneity of GABA A -Benzodiazepine Receptors....Pages 119-124 The Postsynaptic Glycine Receptor — A Member of the Neurotransmitter-Gated Channel Protein Family....Pages 125-130 Immunological identification of a neonatal glycine receptor isotype and its expression in primary cultures of spinal cord....Pages 131-138 Glycine receptor regulation in rodent spinal cord....Pages 139-148 Expression-Cloning and Analysis of the Serotonin 1c Receptor from Mouse Brain....Pages 149-161 Cloned Serotonin 5HT-2 Receptor: Structure and Second Messenger Coupling....Pages 163-173 An Apparent New L-Glutamate Receptor in the Mammalian-Peripheral Nervous System....Pages 175-179 Molecular Properties of Voltage-Sensitive Sodium and Calcium Channels....Pages 181-200 Genetic and Molecular Analysis of a Gene Encoding Sodium Channels in Drosophila....Pages 201-214 A Family of Potassium Channels from the Shaker Locus of Drosophila ....Pages 215-229 Molecular Basis of Biological Diversity at the Shaker Locus of Drosophila....Pages 231-243 Common Properties of Receptors Coupled to GTP Binding Regulator Proteins....Pages 245-257 Cloning of a Rat D 2 -Dopamine Receptor cDNA....Pages 259-267 Molecular characterization of neuropeptide receptors and of a novel membrane protein involved in K + permeation....Pages 269-277 A 64 kDa protein is a candidate for a TRH receptor in prolactin-producing rat pituitary tumour cells....Pages 279-282 Comparison of the Presynaptic Vesicle Component Synaptophysin and Gap Junction Proteins: A Clue for Neurotransmitter Release?....Pages 283-289 Use of Synthetic Peptides and High Affinity Protein Ligands for Structural Studies of Central and Peripheral Nicotinic Receptors....Pages 291-309 Functional Domains of the Nicotinic Acetylcholine Receptor....Pages 311-320 The Limited Sequence Specificity of Anti-Peptide Antibodies may Introduce Ambiguity in Topological Studies....Pages 321-326 Structure, Conformation and Immunological Analysis of Peptide 188–201, the Putative Binding Site for α-Bungarotoxin in the α Subunit of Acetylcholine Receptor....Pages 327-343 Towards an Understanding and use of the Cholinergic Binding Site....Pages 345-352 Evidence for Multipoint Attachment of Ligands to the Nicotinic Acetylcholine Receptor....Pages 353-359 Fine Structural Characterization of the Main Immunogenic Region of the Nicotinic Acetylcholine Receptor....Pages 361-371 Phosphorylation of the Nicotinic Acetylcholine Receptor and Localization of Its Phosphorylation Sites....Pages 373-380 A New Bromobimane Fluorescent Label for Anion Exchange Proteins....Pages 381-391 Changes in Membrane Phosphoproteins Associated with the ATP Modulation of the Delayed Rectifier K + Channel in the Squid Giant Axon....Pages 393-399 Channel Modelling Experiments in Planar Bilayers Using Amphiphilic Helical Polypeptides....Pages 401-412 Structural and Dynamic Molecular Models for Sodium Channels....Pages 413-440 Processing, Assembly, and Ligand Binding of the Mouse Nicotinic Acetylcholine receptor Subunits in Stably Transfected Fibroblasts....Pages 441-449 Assembly and Insertion of a Multi-Subunit Nicotinic Acetylcholine Receptor into Plasma Membranes....Pages 451-467 Stable Expression of Multisubunit Protein Complexes in Mammalian Cells....Pages 469-480 Regulation of Acetylcholine Receptor Gene Expression During Development of the Neuromuscular Junction....Pages 481-507 Developmental Regulation of Acetylcholine Receptor-Subunit mRNA at the Rat Neuromuscular Junction....Pages 509-517 Myotube Specific Expression of the Mouse AChR Delta Subunit Gene is Conferred by 148 BP of 5′ Flanking DNA....Pages 519-540 Transcriptional Regulation of Acetylcholine Receptor Genes in Transfected Muscle Cells and Transgenic Mice....Pages 541-552 The Intermediate Lobe of the Pituitary: A Model for Studying Mechanisms of Trans-Synaptic Regulation of Specific Gene Expression....Pages 553-563 Positive Modulators of Acetylcholine Receptor: Differences Between Skeletal Muscle and Electric Organ....Pages 565-584 Localisation of Muscarinic Acetylcholine Receptors in Cat Visual Cortex During Postnatal Development....Pages 585-593 Regulation of Cortical Muscarinic Acetylcholine Receptors: Involvement of Potassium Channels....Pages 595-617 Regulation of Calcium Channels and Adenylate Cyclase by Opiates....Pages 619-631 Transdifferentiation to a Neuronal Phenotype in Adult Bovine Chromaffin Cells: Effects of αMSH, bFGF and Histamine....Pages 633-644 Molecular Biology of Neuroreceptors: Implications for Clinical Neuroscience....Pages 645-659 Back Matter....Pages 661-678