Minicircle and Miniplasmid DNA Vectors: The Future of Non-viral and Viral Gene Transfer

Content: Chapter 1 Minicircle Patents: A Short IP Overview of Optimizing Nonviral DNA Vectors (pages 1–6): Martin Grund and Dr. Martin Schleef Chapter 2 Operator–Repressor Titration: Stable Plasmid Maintenance without Selectable Marker Genes (pages 7–21): Rocky M. Cranenburgh Chapter 3 Selection by RNA–RNA Interaction: Maximally Minimized Antibiotic Resistance?Free Plasmids (pages 23–36): Juergen Mairhofer and Reingard Grabherr Chapter 4 Plasmid?Based Medicinal Products – Focus on pFAR: A Miniplasmid Free of Antibiotic Resistance Markers (pages 37–58): Corinne Marie, Mickael Quiviger, Helen Foster, George Dickson and Daniel Scherman Chapter 5 Plasmid DNA Concatemers: Influence of Plasmid Structure on Transfection Efficiency (pages 59–69): Christof Maucksch, Bronwen Connor and Carsten Rudolph Chapter 6 Analytical Tools in Minicircle Production (pages 71–91): Anja Rischmuller, Martina Viefhues, Mareike Dieding, Markus Blaesen, Marco Schmeer, Ruth Baier, Dario Anselmetti and Dr. Martin Schleef Chapter 7 Utilizing Minicircle Vectors for the Episomal Modification of Cells (pages 93–113): Orestis Argyros, Suet?Ping Wong, Charles Coutelle and Richard P. Harbottle Chapter 8 Replicating Minicircles: Overcoming the Limitations of Transient and Stable Expression Systems (pages 115–163): Kristina Nehlsen, Sandra Broll, Raju Kandimalla, Niels Heinz, Markus Heine, Stefanie Binius, Axel Schambach and Jurgen Bode Chapter 9 Magnetofection of Minicircle DNA Vectors (pages 165–176): Flavie Sicard, Cedric Sapet, Nicolas Laurent, Elodie Bertosio, Melanie Bertuzzi and Olivier Zelphati Chapter 10 Minicircle?Based Vectors for Nonviral Gene Therapy: In Vitro Characterization and In Vivo Application (pages 177–188): Dennis Kobelt, Jutta Aumann, Dr. Martin Schleef, Marco Schmeer, Ulrike Stein, Peter M. Schlag and Wolfgang Walther Chapter 11 Episomal Expression of Minicircles and Conventional Plasmids in Mammalian Embryos (pages 189–202): Wiebke Garrels, Khursheed Iqbal and Wilfried A. Kues Chapter 12 Tissue?Targeted Gene Electrodelivery of Minicircle DNA (pages 203–213): Sophie Chabot, Muriel Golzio and Justin Teissie Chapter 13 Increased Efficiency of Minicircles Versus Plasmids Under Gene Electrotransfer Suboptimal Conditions: An Influence of the Extracellular Matrix (pages 215–225): Vanessa Joubert, Franck M. Andre, Marco Schmeer, Dr. Martin Schleef and Lluis M. Mir This first title on the topic provides complete coverage, including the molecular basis, production and possible biomedical applications. Written by the most prominent academic researchers in the field as well as by researchers at one of the world's leading companies in industrial production of minicircle DNA, this practical book is aimed at everyone who is directly or indirectly involved in the development of gene therapies. * Covers a new approach to gene therapy with a greatly improved safety profile * Complete coverage of the topic including molecular basis, production and biomedical applications * Has a wide audience including everyone who is directly or indirectly involved in the development of gene therapies .