Microorganisms in industry and environment : from scientific and industrial research to consumer products : proceedings of the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009), Lisbon, Portugal, 2-4 De

This book aims to disseminate the most current research in applied microbiology presented at the III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009) held in Lisbon, Portugal, in December 2009. This volume offers an inviting exploration of microbiology from scientific and industrial research to consumer products in a compilation of more than 150 papers written by leading experts in the field, who afford critical insights into several topics, review current research and discuss future directions to stimulate further discussions. This book also serves as an update on the most important current microbial research, by providing a comprehensive overview of cutting-edge topics in a single volume, where readers can also gain insights into how microbiology can solve problems in everyday settings. Although largely intended for microbiologists interested in knowing the latest developments in agriculture, environmental, food, industrial, medical and pharmaceutical microbiology and microbial biotechnology, this book is also a great source of reference for scientists and researchers involved in advancements in applied microbiology. Contents......Page 8 Introduction......Page 6 Agriculture and Soil Microbiology......Page 18 2. Materials and Methods......Page 20 3.2 Effects of exposure at 14oC or 24oC on R. solanacearum survival in environmental water microcosms......Page 21 4. Discussion......Page 22 References......Page 23 2.1 Experimental site......Page 25 2.4 Microbial community structure by 16S rRNA gene library analysis......Page 26 Acknowledgements......Page 27 References......Page 28 1. Introduction......Page 29 2.4 Characterization of chitosan......Page 30 3. Results and Discussion......Page 31 References......Page 32 2.1 Microorganisms and growth conditions......Page 33 3.1 Native plasmids presence in Pss isolated from mango......Page 34 3.2 Mangotoxin influence in epiphytic fitness of Pss......Page 35 References......Page 36 1. Introduction......Page 38 3. Results and Discussions......Page 39 References......Page 41 1.2 Structure and splicing......Page 43 3. Results......Page 44 References......Page 46 2. Materials and Methods......Page 48 3. Results......Page 49 References......Page 52 1. Introduction......Page 53 2.2 Experiment conditions......Page 54 3. Results......Page 55 4. Discussion......Page 56 References......Page 57 1. Introduction......Page 58 3. Results and Discussion......Page 59 References......Page 61 2.3. Bioassay procedure......Page 62 4. Results and Discussion......Page 63 References......Page 64 2.1 Bacterial strain, plant material and growth conditions......Page 65 4. Discussion......Page 66 References......Page 67 1. Introduction......Page 68 3.2 E. amylovora viability under different stress conditions......Page 69 Acknowledgements......Page 70 References......Page 71 1. Introduction......Page 72 3. Results and Discussion......Page 73 References......Page 75 2.2 PCR conditions......Page 76 References......Page 77 2. Materials and Methods......Page 78 3. Results......Page 79 References......Page 81 2.1 Plant growth and soil amendment......Page 83 3. Results......Page 84 4. Discussion......Page 86 References......Page 87 2. Materials and Methods......Page 88 3.1. Nitrogen leaching below the root zone depth......Page 89 References......Page 92 1. Introduction......Page 93 2.4 Identification of fungal laccase in roots of white mustard......Page 94 3.2 Oxidative and peroxidative activities in soil......Page 95 References......Page 96 1. Introduction......Page 98 2.4 Statistical analysis......Page 99 3.2 Pratylenchus goodeyi mortality and mobility in Solanum sisymbriifolium and S. nigrum extracts......Page 100 Acknowledgements......Page 101 References......Page 102 2. Materials and Methods......Page 103 3. Results and Discussion......Page 105 References......Page 107 2.1 Bacterial strains......Page 109 3.2 Extension of the period of bacterial culturability by the addition of the amylovoran extract......Page 110 References......Page 111 2.2. Phenotypic assays......Page 113 4. Results......Page 114 6. Discussion......Page 115 References......Page 116 2.1 Isolates origin and identification......Page 118 2.2 Pathogenicity tests......Page 119 3.2 Inoculation on cut stems......Page 120 References......Page 122 2.1 Bacterial strains used in this research......Page 123 3.1 Isolation and identification of onion-derived pathogens......Page 124 3.3 Determination of Serratia-related rDNA in total onion DNA......Page 125 4. Discussion......Page 126 References......Page 127 2. Materials and Methods......Page 128 3.1 Substrate utilization by PPO from C. cibarius......Page 129 3.3 Effect of inhibitors on PPO activity in C. cibarius extract and kinetics parameters......Page 130 4. Discussion......Page 131 References......Page 132 2. Materials and Methods......Page 133 3. Results and Discussion......Page 134 References......Page 136 2.1 Yeast strain and supplies......Page 138 3. Results and Discussion......Page 139 References......Page 141 1. Introduction......Page 142 2. Materials and Methods......Page 143 References......Page 144 2. Materials and Methods......Page 146 3. Results......Page 147 References......Page 148 2. Materials and Methods......Page 149 3. Result and Discussion......Page 150 References......Page 152 2. Materials and Methods......Page 154 3. Results and Discussion......Page 155 References......Page 156 Environmental Microbiology......Page 158 1. Introduction......Page 160 2. Materials and Methods......Page 161 3.2 Irradiation of model bacteria......Page 162 3.2 Effect of bacterial inoculation on morpho-physiological parameters of irradiated plants......Page 163 References......Page 164 2. Materials and Methods......Page 165 4. Discussion......Page 168 References......Page 169 2.2 PCR and sequencing of 16S rRNA......Page 170 3.2 Identification of nocardioform actinomycetes......Page 171 4. Conclusion......Page 172 References......Page 174 1. Introduction......Page 175 2.4 Analytical methods......Page 176 3. Results and Discussion......Page 177 4. Conclusions......Page 178 References......Page 179 2. Materials and Methods......Page 180 3.3 Liquid holding recovery in phosphate buffer saline......Page 181 4. Ultrastructural Studies......Page 182 References......Page 183 1. Introduction......Page 184 2. The Individual-Based Model INDISIM-SOM......Page 185 3. Results and Discussion......Page 187 References......Page 188 2.1 Fungal isolates......Page 189 3. Results and Discussion......Page 190 References......Page 191 1. Introduction......Page 193 2.3. Chitosan characterization......Page 194 3.1. Biomass and chitosan production by Absidia corymbifera......Page 195 4. Conclusion......Page 196 References......Page 197 2.1 Sample preparation and irradiation conditions......Page 198 2.2 Oxidative stress......Page 199 3. Results and Discussion......Page 200 References......Page 201 3. Results and Discussions......Page 202 References......Page 203 2.1 Study area......Page 204 2.3 Toxicity tests......Page 205 3.2 Toxicity tests......Page 206 References......Page 207 2.1 Bacteria......Page 209 References......Page 210 2. Materials and Methods......Page 212 3. Results and Discussion......Page 213 Acknowledgements......Page 214 References......Page 215 1. Introduction......Page 216 2.2 Determination of proteins, MDA, GSH, GSSG contents and enzymatic activities......Page 217 3. Results and Discussion......Page 218 References......Page 220 2. Materials and Methods......Page 221 3.2 Screening of the enzymatic activities......Page 222 3.3 Characterization of the strain KWC4......Page 223 References......Page 224 2.1 Microorganism......Page 226 3. Results and Discussion......Page 227 References......Page 229 1. Introduction......Page 231 2.2 Determination of proteins, MDA, GSH, GSSG contents and enzymatic activities......Page 232 3. Results and Discussion......Page 233 References......Page 234 1. Introduction......Page 236 2.3 Phylogenetic and statistical analyses......Page 237 3.2 Ecological perspective of the Ulleung Basin sediment implicated by the phylogenetic analysis......Page 238 Acknowledgements......Page 241 References......Page 242 2.2 Survey of equilibrium isotherms......Page 244 3. Results and Discussion......Page 245 References......Page 248 Bioremediation......Page 250 1. Bioremediation by symbiotic engineering......Page 252 3. Accumulation of metals in nodules and viable parts of plant by infection of the recombinant strain B3 carrying AtPCS and AtIRT1......Page 253 4. Practical strategy for Cd removal from contaminated soils......Page 254 References......Page 255 1. Introduction......Page 256 3. Results and Discussion......Page 257 References......Page 259 1. Introduction......Page 261 3. Results and Discussion......Page 262 References......Page 263 2. Materials and Methods......Page 265 3. Results and Discussion......Page 266 References......Page 268 1. Introduction......Page 269 3.1 Degradation of PAH during two-step bioremediation treatment......Page 270 4. Discussion......Page 272 References......Page 273 1. Introduction......Page 274 3. Results and Discussion......Page 275 References......Page 277 2. Materials and Methods......Page 278 3.2 Photolysis of MCPA......Page 279 3.3 Biodegradation of pre-irradiated MCPA......Page 280 References......Page 281 2.1 Kinetics of uranium biosorption......Page 282 3.2 Adsorption isotherms......Page 283 3.3 Adsorption by live and dead mycelium......Page 284 4. Discussion......Page 285 References......Page 286 Biofilms......Page 288 2.1 Experimental design......Page 290 3.1 Structure of microbial communities......Page 291 3.2 Activity of microbial communities......Page 292 References......Page 293 2.1 Area of study......Page 294 3. Results and Discussion......Page 295 3.3 Protein estimation......Page 296 References......Page 297 2.1 Strains......Page 298 4. Detection of the icaA, icaC and icaD Genes Specific for Biofilm Production......Page 299 6.1. Study of biofilm production......Page 300 7. Discussion......Page 301 References......Page 302 2.1 Origin of isolates......Page 303 4. Discussion and Conclusion......Page 304 References......Page 305 Industrial Microbiology......Page 306 1. Introduction......Page 308 3. Results and Discussion......Page 309 References......Page 310 1. Introduction......Page 311 3. Results and Discussion......Page 312 References......Page 314 Biosynthesis of the lipids by the yeasts Rhodotorula gracilis......Page 315 References......Page 319 1. Introduction......Page 320 2.2 Culture media......Page 321 3. Results and Discussion......Page 322 4. Conclusions......Page 323 References......Page 324 2. Materials and Methods......Page 325 3. Results and Discussion......Page 327 References......Page 329 2.1. Test strains of bacteria......Page 330 3.1. Effect of inulin on probiotic bacteria......Page 331 3.2. Effect of oligofructose on probiotic bacteria......Page 332 3.4. Determining the effect of oxycellulose at varying pH......Page 333 References......Page 334 2.1 Microorganism......Page 335 3.1 Effect of pH on growth and pigment production by Dietzia maris......Page 336 3.3 Statistical analysis......Page 337 Acknowledgements......Page 338 References......Page 339 2.1 Isolation of cellulase producing bacteria......Page 340 3. Results and Discussion......Page 341 References......Page 344 2.1 Fungal strain......Page 345 2.6 Experimental design and statistical analysis......Page 346 3.2 Mixture design for three substrates (orange peel, corncob, and coffee husk)......Page 347 References......Page 348 2.2 Protoplasting and transformation of B. megaterium, B. subtilis, and B. cereus......Page 350 3. Result......Page 351 References......Page 352 2.1 Experimental design......Page 354 3. Results and Discussion......Page 355 3.2 Response surface for the concentration factor......Page 356 3.3 Response surface for the purification factor......Page 357 Acknowledgements......Page 358 References......Page 359 1. Introduction......Page 360 2.5. Detection of enzymatic activities......Page 361 3. Results and Discussions......Page 362 References......Page 364 2. Materials and Methods......Page 365 3. Results and Discussion......Page 366 References......Page 368 Food Microbiology......Page 370 2. Materials and Methods......Page 372 3. Results......Page 373 References......Page 375 2. Materials and Methods......Page 376 4. Discussion and Conclusion......Page 377 References......Page 379 3. Results and Discussion......Page 380 References......Page 382 2.1. Organisms, media and culture conditions......Page 383 2.3. Biocontrol “in apples” assays......Page 384 3.2. Biocontrol assays “in apples”......Page 385 Acknowledgements......Page 386 References......Page 387 1. Introduction......Page 389 3.1 Coliform bacteria in fresh vegetables......Page 390 3.2 Resistance patterns......Page 391 References......Page 392 2.1 Fungal strain......Page 394 3.1 Growth rates......Page 395 3.2 OTA accumulation......Page 396 References......Page 397 1. Introduction......Page 398 2.5 Growth monitoring......Page 399 3.1 Mancozeb......Page 400 4. Conclusions......Page 401 References......Page 402 1. Introduction......Page 403 2.6 DNA analysis......Page 404 3.1 Total, culturable and viable cells counts......Page 405 4. Conclusions......Page 406 References......Page 407 2.1 Sampling......Page 408 References......Page 409 2.2 Molecular identification......Page 410 3.1 Molecular identification......Page 411 3.2 Characterization of isolates......Page 412 References......Page 413 2.1 Collection of samples and mold isolation......Page 415 3.1 Mold isolates......Page 416 3.1 Identification of mold isolates......Page 417 3.2 Mycotoxin production......Page 418 References......Page 419 2.1 Bacterial strains and culture conditions......Page 420 3. Results......Page 421 4. Discussion and Conclusions......Page 423 References......Page 424 2. Materials and Methods......Page 425 3. Results......Page 426 Acknowledgements......Page 428 References......Page 429 1. Background......Page 430 3.1 Consumption data......Page 431 3.3 Correlation between consumption of individual foods and OTA levels in plasma......Page 432 Acknowledgements......Page 433 References......Page 434 2.3. Statistical treatment......Page 435 3.2. Volatile compounds......Page 436 3.4. Sensorial analysis......Page 437 References......Page 438 2. Materials and Methods......Page 439 3. Results and Discussion......Page 440 References......Page 441 2.1 Fungal strains......Page 443 2.5 Patulin determination......Page 444 3. Results and Discussion......Page 445 Acknowledgements......Page 446 References......Page 447 2.1 Bacterial strains......Page 448 3.1 Adaptation and strain selection......Page 449 3.2 Microvinifications and sensory analysis......Page 450 3.3 Molecular characterization......Page 451 References......Page 452 1. Introduction......Page 453 3.1 DNA extraction methods......Page 454 3.3 Species diversity......Page 455 References......Page 456 Standardisation and optimisation of the Alkaline-Tolerance Response (AlTR) in Listeria monocytogenes 10403S......Page 458 References......Page 461 2.1 Microorganisms......Page 463 2.5 Study of the influence of different inhibitors on proteolytic systems of LAB......Page 464 3. Results......Page 465 4. Discussion......Page 466 References......Page 467 1. Introduction......Page 468 2.2 Spectrophotometric growth curve......Page 469 3.2 Glucose consumption......Page 470 References......Page 471 Medical and Pharmaceutical Microbilogy......Page 472 1. Introduction......Page 474 3. Results and Discussion......Page 475 References......Page 476 2.1. Animals......Page 477 References......Page 478 1.1 Thyme......Page 480 2. Materials and Methods......Page 481 3. Results and Discussion......Page 482 References......Page 483 1. Introduction......Page 485 3. Results and Discussion......Page 486 Acknowledgements......Page 488 References......Page 489 1. Introduction......Page 490 3.2 Reversal assay......Page 491 References......Page 492 2. Materials and Methods......Page 493 3. Results and Discussion......Page 494 References......Page 495 1. Introduction......Page 496 3.4 Preparation of the polymers......Page 497 3. Results and Discussion......Page 498 References......Page 499 1. Introduction......Page 501 3. Results and Discussion......Page 502 Acknowledgements......Page 503 References......Page 504 2.1 Plant extracts......Page 505 3. Results and Discussion......Page 506 References......Page 507 2. Materials and methods......Page 509 3. Results......Page 510 5. Conclusions......Page 511 References......Page 512 1. Introduction......Page 513 3. Results and Discussion......Page 514 4. Conclusion......Page 516 References......Page 517 2.2 Isolation and storage of the isolates......Page 518 3.1 Isolation of microorganisms......Page 519 3.3 Detection of antimicrobial membrane activity......Page 520 References......Page 521 2.1 Collection of vaginal samples......Page 523 3. Results and Discussion......Page 524 References......Page 526 2. Materials and Methods......Page 527 3. Results and Discussion......Page 528 References......Page 530 1. Introduction......Page 532 3. Results and Discussion......Page 533 References......Page 534 1. Introduction......Page 535 2. Materials and Methods......Page 536 4. Discussion......Page 537 References......Page 538 2. Materials and Methods......Page 539 References......Page 540 2.2 Salmonella enterica serovar Typhimurium (S. typhimurium)......Page 542 4. Extracts......Page 543 5. Results......Page 544 6. Discussion and Conclusion......Page 545 References......Page 546 Biotechnologically Relevent Enzymes and Proteins......Page 548 2.1 Strain and growth conditions......Page 550 3.1 Enzyme (peroxidise and xylanase) production during growth of S. albus in minimal salts medium containing xylan as the main carbon and energy source......Page 551 3.2 Biotechnological potential of actinobacterial peroxidases......Page 552 4.2 Biotechnological potential of actinobacterial peroxidases......Page 553 References......Page 554 1. Introduction......Page 555 3.1 Cultivation time and pectinase production by Cladosporium cladosporioide (Fres.) de Vries......Page 556 3.2 Enzymatic extraction......Page 557 4. Conclusion......Page 558 References......Page 559 2.2 Incubation with different concentration of hbFGF......Page 560 3. Results and Discussion......Page 561 References......Page 562 2.1 Bacterial strains and culture condition......Page 563 3.1 The conventional chloroform-methanol extraction resulted in distinct CL signals among strains that are correlated with the ASABF-α resistance......Page 564 3.2 The cls mutation did not affect the ASABF-α susceptibility......Page 565 References......Page 566 1. Introduction......Page 567 2.7 Halotolerance and inhibition assays......Page 568 3.2 Decolourisation and detoxification of an azo dye by the laccase produced by S. ipomoea......Page 569 References......Page 570 2.1 Primer designing and site directed mutagenesis by PCR......Page 572 3.1 Construction of pET-1010......Page 573 4. Discussion......Page 574 References......Page 575 1. Introduction......Page 576 2.4 Ligninolytic Enzyme......Page 577 3. Results......Page 578 References......Page 579 2. Materials and Methods......Page 581 3. Results and Discussion......Page 582 References......Page 584 Microfactories — Microbial Production of Chemicals and Pharmaceuticals......Page 586 2.2 Detection of bacteriocin production......Page 588 3. Results and Discussion......Page 589 References......Page 591 2.1 Strain......Page 592 3.3 Measurement of hydrogen sulfide......Page 593 Acknowledgements......Page 594 References......Page 595 1. Introduction......Page 596 2.5 Molecular weight......Page 597 3. Results and Discussion......Page 598 References......Page 599 1.2 Downstream processing overview for clavulanic acid......Page 601 2.2 Methods......Page 602 3.1 Clavulanic acid liquid-liquid extraction assays......Page 603 4. Conclusion......Page 604 References......Page 605 2.2. Crude extracts production and quantification of antimicrobial activity......Page 606 3. Results and Discussion......Page 607 References......Page 608 2.2 Bioactivity test......Page 610 3. Results and Discussion......Page 611 References......Page 612 2.2 Isolation of samples......Page 613 3. Results and Discussion......Page 614 References......Page 615 2.1 Materials......Page 617 3.1 Transformation of nerol with A. niger AHU7120 in Czapeck-Dox medium......Page 618 References......Page 621 1. Introduction......Page 622 3.1 Sulfuric acid hydrolysis of pistachio shells......Page 623 3.2 Removal of inhibitors......Page 624 3.3 Fermentation of acid hydrolysate......Page 625 References......Page 626 Microbial Physiology, Metabolism and Gene Expression......Page 628 2.1 Yeast strains......Page 630 3.1 Characterisation of YARE on KlHIS4 in the response to cadmium......Page 631 3.2 Involvement of the YARE-like element in the F2 gel-shift pattern......Page 632 3.3 YARE-dependent response was not produced with S. cerevisiae protein extracts......Page 633 References......Page 634 2. Materials and Methods......Page 636 3.1 Effect of Ni2+, Cd2+ and Hg2+ on S. typhimurium growth......Page 637 3.2 Effect of Ni2+, Cd2+ and Hg2+ on S. typhimurium cytochrome content......Page 638 4. Discussion......Page 639 References......Page 640 1. Introduction......Page 641 2.4 Induction and Isolation of Mutants......Page 642 3.1 The single treatment......Page 643 References......Page 645 1. Introduction......Page 647 3. Results and Discussions......Page 648 References......Page 650 2. Materials and Methods......Page 652 3.1 Effect of cyanide and azide on cell growth and cytochromes content......Page 653 3.3 Kinetics studies of the effect of NaN3 on the binding of cyanide to cytochrome d......Page 654 4. Discussion......Page 655 References......Page 656 2.1 Yeast strains, media and cultivation......Page 657 3.1 Regulation of AOD synthesis in mutants mth......Page 658 3.2 САТ and respiratory activity of the mth mutants......Page 659 References......Page 661 2. Materials and Methods......Page 662 3.2 Outer chain elongation of N-linked oligosaccharides......Page 663 References......Page 664 2.2 Chemicals......Page 666 4. Discussion......Page 667 References......Page 669 1.2 The yeast PUT2 gene......Page 671 3.1 Characterization of the KlADE2 gene in Kluyveromyces lactis......Page 672 3.2 The KlPUT2 gene from Kluyveromyces lactis......Page 674 References......Page 675 2. Materials and Methods......Page 676 3.2 Neutral red can be used to monitor acidification in vacuole-related studies......Page 677 References......Page 678 Methods......Page 680 1. Introduction......Page 682 4. Conclusions......Page 683 References......Page 684 2.1. Interacting molecules and AmB fragments......Page 685 3.1. Dividing and fragments obtained from breaking AmB......Page 686 3.3. Result of antifungal bioassay......Page 687 References......Page 688 2. Second-order Gompertzian kinetics......Page 689 3. Nonlocality......Page 690 4. Coherence......Page 691 6. Generalization......Page 692 References......Page 693 2.1 Experimental design......Page 695 2.3 Neural network models......Page 696 3.1 General trends......Page 697 3.3 RBFN models......Page 698 References......Page 699 1.1 The bacterial cell cycle......Page 700 2.2 The C and D periods determination errors......Page 701 2.3 Determining the accuracy of the D period estimation......Page 702 References......Page 703 2.1 Instrumentation......Page 705 3.2 Influence of potential......Page 706 3.5 Calibration dependence......Page 707 References......Page 708 2.1 Chemicals and reagents......Page 710 3.1 Lag phase......Page 711 3.3 PAT accumulation......Page 712 References......Page 714 1. Introduction......Page 716 5. Lessons from Literature Based Refinements......Page 717 References......Page 719 2. Materials and Methods......Page 721 3.1 Thin section......Page 722 3.2 Freeze fracture......Page 723 4. Discussion......Page 724 References......Page 725 2.1 In silico methods......Page 726 4. Discussion......Page 727 References......Page 728 2.1. Optimization of the structures and calculation procedures......Page 730 3. Results and Discussion......Page 731 References......Page 734 Agriculture and soil microbiology. Ability of Ralstonia solanacearum phylotype II to adapt to environmental prevailing factors in water / Belen Alvarez, Maria M. Lopez and Elena G. Biosca. Analysis of microbial communities under long-term conventional and reduced-input management of tillage soil / Sagar Chhabra [und weitere]. Antimicrobial activity of chitosan against Fusarium oxysporum f. sp. tracheiphilum / T.C.M. Stamford [und weitere]. Aspects about virulence and epiphytic fitness of Pseudomonas syringae pv. syringae strains isolated from mango trees / E. Arrebola [und weitere]. Characterization of rhizospheric bacteria isolated from maca (Lepidium meyenii W.) in the highlands of Junin-Peru / D. Zuniga Davila [und weitere]. Detection of group I and group II introns in a Mexican Bacillus thuringiensis collection / A. Espino-Vazquez [und weitere]. Determination of soil microbial community fluctuations by different techniques in a maize field / Ana Munoz [und weitere]. Differences between microbial communities in worm guts and the soils they inhabit / R.M. Simpson, M. Picquet and M. Deurer. Effect of different rhizospheric bacteria in the growth of Gossypium barbadense L. in Peru / D. Yino Oshiro [und weitere]. Effect of in vivo passage on spore germination and virulence of Verticillium lecanii / Ali Derakhshan. Erwinia amylovora colonization of host plants inoculated by irrigation / R.D. Santander [und weitere]. Evaluation of flow cytometry to assess Erwinia amylovora viability under different stress conditions / R.D. Santander [und weitere]. Genetic variability analysis of entomopathogenic fungi isolated from citrus-growing areas of Mexico / A.L. Galan-Franco [und weitere]. Identification of virulence genes in Fusarium oxysporum f. sp. lycopersici the causal agent of tomato wilt disease / M.L.R. Bastos da Silva [und weitere]. Influence of organic and conventional soil management system on soil respiration and enzymatic activity / L. Dubova [und weitere]. Microbial analysis of soils from avocado crop modified by organic amendments / N. Bonilla [und weitere]. Modeling of nitrogen leaching by using urea fertilizer in sandy loam soil / Khalil Ajdary and Hamid Zare Abianeh. Mutual influence of the non-symbiotic soil basidiomycete, Stropharia rugoso-annulata and white mustard plants on their enzymatic activities under natural conditions / G. Gramss and H. Bergmann. Nematicidal activity of Solanum sisymbriifolium and S. nigrum extracts against the root-lesion nematode Pratylenchus goodeyi / M. Pestana [und weitere]. Particular characteristics of soil microbial communities in forest stands infected with Heterobasidion parviporum and Armillaria spp. / L. Grantina [und weitere]. Pathogenic bacteria can produce exopolysaccharides and use them as carbon source under stress conditions : the case of Erwinia amylovora / M. Ordax [und weitere]. Phenotypic characterization and the application of the rep-PCR technique in a study of new strains of Bacillus thuringiensis in the South of Brazil / G. Cristina Alles, V. Machado and L. Mariana Fiuza. Phytophthora parasitica showing host specificity and pathogenic ability on tomato and sweet pepper / M. de Cara [und weitere]. Polybiotrophy of Serratia marcescens, a causative agent of an onion disease in arid zone of the South of Ukraine / L. Ovcharenko [und weitere]. Polyphenol oxidase in golden chanterelle (Cantharellus cibarius) mushroom / Jacqueline Keyhani and Ezzatollah Keyhani. Prevalence of plant pathogenic isolates of airborne Fusarium species in south east coast of Spain / D. Palmero [und weitere]. Production of beer using sorghum and sorghum malt / P.J. Segura [und weitere]. Sequencing of the region of ribosomal internal transcribed spacer (ITS) of Metarhizium anisopliae in Pernambuco state / M.L.R.B da Silva [und weitere]. Soil bioremediation of atrazine pesticide by two strains of soil microorganism / Ana Munoz [und weitere]. Study on the effects of nitrogen, glucose and plant residues on soil microbial C / S. Shahsavani and G. Shakeri. The toxicity and histopathology of Bacillus thuringiensis Cry1Ba toxin to Spodoptera frugiperda (Lepidoptera, Noctuidae) / L. Massochin Nunes Pinto, N. Carolina Drebes Dorr and L. Mariana Fiuza -- Environmental microbiology. A bacterial consortium alleviated a low-dose gamma-irradiation effect in Kalanchoe plantlets / O.P. Boorlak [und weitere] Isolation And Antimicrobial Activity Of Streptomyces Tubercidicus Against Pathogenic Bacteria And Fungi / R. Priscila Ratti ... [et Al.]. Isolation, Phenogenotypic Idenfication And Bioactivity Evaluation Of Endophytic Microorganisms Intrinsically Associated With Miconia Albicans In Brazilian Tropical Savannah Tree In Sao Carlos, Sp / A. C. M. Toledo Piza ... [et Al.]. Transformation Of Nerol With Aspergillus Niger In Czapek-dox Medium / Takehiko Tsuruta. Xylitol Production From Dilute-acid Hydrolysis Of Bean Group Shells / Chizuru Sasaki ... [et Al.] -- Microbial Physiology, Metabolism And Gene Expression. A Yare-like Element As The Binding Site For Specific Klhis4 Transcriptional Regulators And Its Implication In Cadmium Toxicity / M. Lamas-maceiras And M. A. Freire-picos. Alterations Induced By Transition Metals Nickel, Cadmium And Mercury In Salmonella Typhimurium Growth And Membrane Proteins / Hossein Tayefi-nasrabadi And Ezzatollah Keyhani. Assaying The Single And Combined Genotoxicity Of Calotropis Procera Ait Latex And Chlorcyrin In Aspergillus Terreus / Jamal S. M. Sabir. Construction Of A Plasmid Vector For Thermoacidophilic Crenarchaeon Sulfolobus Acidocaldarius / Yoko Kawasaki And Norio Kurosawa. Effect Of Cyanide And Azide On Growth And Membrane Proteins In Salmonella Typhimurium / D. Minai-tehrani ... [et Al.]. Influence Of The Mth Genes On Synthesis Of Alcohol Oxidase And Catalase In Methylotrophic Yeasts Pichia Methanolica / E. P. Isakova, Y. I. Deryabina And O. A. Leonovich. Mobility Of Stv1p And Vph1p, The A Subunit Isoforms Of The Saccharomyces Cerevisiae Vacuolar Atpase Through Late Secretory Pathway Membranes / I. Corbacho ... [et Al.]. The Genotoxicity Of Three Synthetic Pesticides : Chlorpyrifos, Cypermethrin And Their Mixture Chlorcyrin In Aspergillus Terreus / Jamal S. M. Sabir. Transcriptional Regulation Of Ade2 And Put2 Genes In Kluyveromyces Lactis / G. Ruiz Moran, M. Lamas Maceiras And A. M. Rodriguez Torres. Yeast Vacuole Staining Using Quinacrine And Neutral Red / I. Corbacho ... [et Al.] -- Methods. A Cheminformatics Approach To An Enriched Database Of Antimycobacterial Compounds Useful For Drug Discovery Pipeline / S. Sardari, B. Shaghaghi And H. Borna. Aggregation-based In Silico Study For Better Understanding Of Related Membrane Interfering Analogous Of Amphotericin B / S. Sardari, S. Mostaan And P. Azerang. Bacterial Growth As Nonlocal Coherent Phenomenon / Marcin Molski. Capacity Of Neural Network Models To Predict Deoxynivalenol Build-up In Barley Grain Contaminated In Vitro With Fusarium Culmorum / F. Mateo ... [et Al.]. Determination Of Cell Cycle Parameters By Flow Cytometry In Bacteria : Practical Considerations / F. Molina ... [et Al.]. Determination Of Ethanol In Alcoholic Drinks Using An Enzyme Biosensor Containing Alcohol Dehydrogenase / V. Polan And K. Vytras. Growth Of Penicillium Expansum And Production Of Patulin In Potato-glucose-agar Medium Supplemented With Imazalil / F. M. Valle-algarra ... [et Al.]. Metabolic Reconstruction Of Synechococcus Elongatus Towards A Minimal Photoautotrophic Cell / C. M. Gonzalez-domenech ... [et Al.]. Nanocurvatures And Nanocup Formation Induced By Ethidium Bromide In Yeast Plasma Membrane / Ezzatollah Keyhani. Selectivity-refined In Silico Analogue Finding Method For New Antifungal Molecules Based On Amphotericin B Molecular Features / M. Ferdosiyan And S. Sardari. Structure Activity Relationship Study Of The [symbol]-lactamase Inhibitors And Theoretical Design For New Bioactive Compounds / Ana Claudia Granato ... [et Al.]