میکروسیال‌ها برای بیوتکنولوژی (سیستم‌های میکروالکترومکانیکی)

The application of microfluidics to biotechnology is an exciting new area that has already begun to revolutionize how researchers study and manipulate macromolecules like DNA, proteins and cells in vitro and within living organisms. Presenting an enlightening balance of numerical approaches, theory, and experimental examples, this cutting-edge resource offers keen insight into this burgeoning field. Supported with over 800 equations and more than 340 illustrations, the book provides a detailed look at the mechanical behavior of the different types of micro/nano particles and macromolecules that are used in biotechnology. You gain a solid understanding of microfluidics theory and the mechanics of microflows and microdrops. The book examines the diffusion of species and nanoparticles, including continuous flow and discrete Monte-Carlo methods. This unique volume describes the transport and dispersion of biochemical species and particles. You learn how to model biochemical reactions, including DNA hybridization and enzymatic reactions. Moreover, the book helps you master the theory, applications, and modeling of magnetic beads behavior and provides an overview of self-assembly and magnetic composite. Other key topics include the electric manipulation of micro/nanoparticles and macromolecules and the experimental aspects of biological macromolecule manipulation. Microelectromechanical systems Microfluidics for Biotechnology 1 Contents vii 7 Preface xiii 13 Acknowledgments xvii 17 Chapter 1 Microflows 1 19 1.1 Introduction 1 19 1.2 Single-Phase Microflows 2 20 1.3 Multiphase Microflows and Liquid Plugs 36 54 1.4 Conclusion 47 65 References 47 65 Chapter 2 Microdrops 51 69 2.1 Introduction 51 69 2.2 The Physics of Wetting 52 70 2.3 The Physics of Drops 68 86 2.4 The Example of Electrowetting 79 97 2.5 Conclusion 85 103 References 86 104 Chapter 3 Diffusion of Nanoparticles and Biochemical Species 89 107 3.1 Introduction 89 107 3.2 Brownian Motion 89 107 3.3 Macroscopic Approach: Concentration 90 108 3.4 Microscopic (Discrete) Approach 111 129 3.5 Conclusion 124 142 References 125 143 Chapter 4 Transport of Nanoparticles and Biochemical Species 127 145 4.1 Introduction 127 145 4.2 Advection-Diffusion Equation 127 145 4.3 Trajectory Calculation 161 179 4.4 Separation/Purification of Bioparticles 168 186 4.5 Conclusion 172 190 References 175 193 Chapter 5 Biochemical Reactions in Biochips 177 195 5.1 Introduction 177 195 5.2 From the Principle of Biorecognition to the Development of Biochips 177 195 5.3 Biochemical Reactions 182 200 5.4 Biochemical Reactions in Microsystems 201 219 5.5 Conclusion 231 249 References 232 250 Chapter 6 Experimental Approaches to Microparticle-Based Assays 235 253 6.1 A Few Biological Targets 235 253 6.2 Biotechnological Tools 242 260 6.3 Experimental Methods of Characterization 250 268 6.4 Micromanipulation 262 280 References 265 283 Selected Bibliography 266 284 Chapter 7 Magnetic Particles in Biotechnology 267 285 7.1 Introduction 267 285 7.2 Characterization of Magnetic Beads 272 290 7.3 Magnetic Force 273 291 7.4 Deterministic Trajectory 274 292 7.5 Example of a Ferromagnetic Rod 277 295 7.6 Magnetic Repulsion 281 299 7.7 The Example of a Separation Column 281 299 7.8 Concentration Approach 283 301 7.9 The Example of Magnetic Field Flow Fractionation (MFFF) 287 305 7.10 Assembly of Magnetic Beads—Magnetic Bead Chains 292 310 7.11 Magnetic Fluids 296 314 7.12 Magnetic Micromembranes 299 317 7.13 Conclusion 303 321 References 304 322 Chapter 8 Micromanipulations and Separations Using Electric Fields 307 325 8.1 Electrophoresis 307 325 8.2 Dielectrophoresis 319 337 8.3 Electro-Osmosis 334 352 References 336 354 Chapter 9 Conclusion 339 357 About the Authors 341 359 Index 343 361 Biotechnology; Microfluidics; Artech House,INC. "The application of microfluidics to biotechnology is an exciting new area that has already begun to revolutionize how researchers study and manipulate macromolecules, like DNA and proteins, and cells in vitro and within living organisms. Presenting an enlightening balance of numerical approaches, theory, and experimental examples, this cutting-edge resource offers keen insight into this burgeoning field. Supported with over 800 equations and more than 340 illustrations."--Jacket Presenting a balance of numerical approaches, theory, and experimental examples, this resource offers insights into the application of microfluidics to biotechnology. Supported with over 800 equations and more than 340 illustrations, it is useful for engineers, laboratory researchers, as well as students in the field.