Microcompartmentation

Cover 1 Title 2 Copyright 3 PREFACE 4 THE EDITOR 6 CONTRIBUTORS 7 TABLE OF CONTENTS 9 Dedication 10 Chapter 1: On the Internal Environment of Animal Cells 12 Chapter 2: Functional Compartmentation of Carbohydrate Metabolism 28 Chapter 3: Mitochondrial Distribution and O2 Gradients in Mammalian Cells 48 Chapter 4: Involvement of Microcompartmentation in the Regulation of Cell Proliferation 66 Chapter 5: Diffusion and Ultrastructural Adaptive Responses in Ectotherms 82 Chapter 6: Microcompartmentation of Metabolite Transport in Mitochondria 104 Chapter 7: The Membranes Involved in Proton-Mediated Free-Energy Transduction: Thermodynamic Implications of their Physical Structure 126 Chapter 8: Microcompartmentation of DNA Precursors 166 Chapter 9: Function of Ambiquitous Proteins in a Heterogeneous Medium 182 Chapter 10: Microzonation of ATP and pH in the Aqueous Cytoplasm of Mammalian Cells 202 Chapter 11: Hydrogen and Calcium Ion Diffusion in Axoplasm 220 Chapter 12: Fluorescence Digital Imaging Microscopy — Spatial Distribution of Ca2+ and H+ in Single Cells 238 Index 264 "Microcompartmentation refers to nonhomogeneous distribution of solutes in compartments of cells or associated structures without intervening membranal barriers. Such variations in concentration of ions, metabolites, intracellular messengers, and nutrients can introduce significant heterogeneity in subcellular function and regulation. The current focus on biological examples of microcompartmentation provides ample evidence for its importance and the role of physical structure in determining local chemical environments. The examples present in the different chapters include microcompartmentation of Ca2+, H+, ATP. ADP, O2, glycolytic intermediates, fatty acids, amino acids, and nucleic acid precursors. In reviewing these systems, the authors provide a useful resource for experimental approaches to study microcompartmentation and provide the basis for future studies of its role in regulation of cell functions."--Provided by publisher