Microbeam analysis in biology : proceedings of a Workshop on Biological X-ray Microanalysis by Electron Beam held in Boston, Mass., Aug. 25-26, 1977

Microbeam Analysis in Biology contains the proceedings of a workshop on Biological X-Ray Microanalysis by Electron Beam Excitation, held in Boston, Massachusetts on August 25-26, 1977. This book focuses on the principles, techniques, and biological use of electron probe microanalysis, energy-loss spectroscopy, and ion probe microanalysis. This text reflects the emphasis of the workshop on presenting the principles of analysis, the optimization of operating conditions, the description of successful techniques for sample preparation and quantitation, the illustration of problems and pitfalls, and the direction of microbeam analysis in biology. Content: Front Matter , Page iii Copyright , Page iv Speakers , Pages ix-xi Participants , Pages xiii-xviii Preface , Pages xix-xx ANALYTICAL TRANSMISSION ELECTRON MICROSCOPY IN BIOLOGY , Pages 1-17 , D.R. Beaman FACTORS AFFECTING X-RAY SENSITIVITY IN ELECTRON MICROBEAM INSTRUMENTATION , Pages 19-37 , Eric Lifshin X-RAY SPECTRAL ARTIFACTS ENCOUNTERED IN THE SEM AND STEM , Pages 39-52 , Roger B. Bolon ELECTRON ENERGY LOSS SPECTROSCOPY (ELS) WITHIN THE ELECTRON MICROSCOPE. WHERE ARE WE? , Pages 53-63 , Michael Isaacson QUANTITATION AND DETECTION LIMITS IN ELECTRON ENERGY LOSS SPECTROSCOPY (EELS) OF THIN BIOLOGICAL SECTIONS , Pages 65-86 , C. Colliex, P. Trebbia A PRACTICAL ELECTRON ENERGY LOSS SPECTROMETER , Pages 87-97 , David C. Joy, Dennis M. Maher ELECTRON ENERGY LOSS MICROANALYSIS OF BIOLOGICAL MATERIAL , Pages 99-109 , Dale E. Johnson DISCUSSION OF ELS , Pages 111-115 SECONDARY ION EMISSION MICROANALYSIS: BIOMEDICAL APPLICATIONS , Pages 117-128 , P. Galle, R. Lefevre SECONDARY-ION MASS SPECTROMETRY: FUTURE APPLICATION TO BIOLOGICAL SAMPLES , Pages 129-151 , Margaret S. Burns-Bellhorn GENERAL DISCUSSION , Pages 153-158 QUANTITATIVE MICROPROBE ANALYSIS IN BIOLOGY , Pages 161-169 , R.R. Warner, C.P. Lechene STATISTICS AND X-RAY ANALYSIS , Pages 171-184 , J.I. Goldstein PROBLEMS OF THE CONTINUUM-NORMALIZATION METHOD FOR THE QUANTITATIVE ANALYSIS OF SECTIONS OF SOFT TISSUE , Pages 185-208 , T.A. Hall ELECTRON PROBE MICROANALYSIS OF THIN SAMPLES , Pages 209-224 , R. Tixier A CATALOGUE OF ARTIFACTS OBSERVED IN ENERGY-DISPERSIVE X-RAY SPECTROMETRY AND THEIR INFLUENCE ON ANALYSIS , Pages 225-263 , C.E. Fiori, R.L. Myklebust, D.E. Newbury QUANTITATIVE ELECTRON PROBE ANALYSIS OF BIOLOGICAL THIN SECTIONS , Pages 265-276 , Henry Shuman ELECTRON PROBE ANALYSIS OF LIQUID DROPLETS , Pages 279-297 , C.P. Lechene, R.R. Warner X-RAY MICROANALYSIS OF RED BLOOD CELLS , Pages 299-316 , R. Gary Kirk, Ping Lee, T.G. Duplinsky, D.C. Tosteson SODIUM AND POTASSIUM MICROPROBE ANALYSIS OF ISOLATED HUMAN RED BLOOD CELLS OR RED BLOOD CELL RESEALED GHOSTS: A COMPARISON OF RESULTS BETWEEN FREEZE-DRIED OR FROZEN-HYDRATED CELLS , Pages 317-325 , Nicole Roinel, Alain Desportes ENERGY-DISPERSIVE X-RAY ANALYSIS OF BIOLOGICAL MICRODROPLETS , Pages 327-345 , Paul M. Quinton APPLICATION OF THE ELECTRON MICROPROBE TO ANALYSIS OF DEVELOPING FROG OOCYTES , Pages 347-358 , Theodore J. Century, Lawrence G. Palmer, Mortimer M. Civan ELECTRON PROBE MICROANALYSIS OF FROZEN-HYDRATED BULK SPECIMENS: BASIC EXPERIMENTS , Pages 361-373 , W. Fuchs THE ELECTRON MICROPROBE X-RAY ANALYSIS OF FROZEN-HYDRATED SECTIONS WITH NEW INFORMATION ON FLUID TRANSPORTING EPITHELIA , Pages 375-408 , Brij L. Gupta ELECTRON PROBE ANALYSIS OF FROZEN-HYDRATED BULK TISSUES , Pages 409-426 , Claude P. Lechene, Joseph V. Bonventre, Ronald R. Warner FREEZING OF MUSCLE FIBERS ULTRATHIN CRYOCUTTING AND TRANSFER OF FROZEN-HYDRATED SECTIONS , Pages 427-444 , Michael Sjöström, Ugo Valdrè ELECTRON PROBE MICROANALYSIS: THE STUDY OF NONDIFFUSIBLE ELEMENTS ON ULTRATHIN SECTIONS , Pages 447-456 , P. Galle X-RAY MICROANALYSIS OF DIFFUSIBLE AND NONDIFFUSIBLE ELEMENTS IN ULTRATHIN BIOLOGICAL TISSUES USING HUMAN SPERM CELLS AS A MODEL FOR INVESTIGATING SPECIMEN PREPARATION , Pages 457-477 , J.A. Chandler, S. Battersby CYTOCHEMICAL/ANALYTICAL STUDIES OF BIOGENIC AMINES IN ULTRATHIN PREPARATIONS , Pages 479-488 , Joe G. Wood RENAL NA-K-ATPase: QUANTITATIVE X-RAY MICROANAYSIS , Pages 489-505 , Seymour Rosen, R. Beeuwkes III ELECTRON PROBE X-RAY MICROANALYSIS AND THE CELLULAR BASIS OF TRANSEPITHELIAL CALCIUM TRANSPORT , Pages 509-515 , James R. Coleman QUANTITATIVE DETERMINATION OF CELLULAR ELECTROLYTE CONCENTRATIONS IN THIN FREEZE-DRIED CRYOSECTIONS USING ENERGY-DISPERSIVE X-RAY MICROANALYSIS , Pages 517-534 , Roger Rick, Adolf Dörge, Klaus Gehring, Richard Bauer, Klaus Thurau CRYOULTRAMICROTOMY FOR ELECTRON PROBE ANALYSIS , Pages 535-555 , A.V. Somlyo, J. Silcox MITOCHONDRIAL CALCIUM CONTENT IN VASCULAR SMOOTH MUSCLE , Pages 557-570 , A.P. Somlyo, B.F. Sloane, A.V. Somlyo, H. Shuman, A. Scarpa QUANTITATIVE ELECTRON PROBE MICROANALYSIS OF ULTRATHIN BIOLOGICAL SECTIONS , Pages 571-590 , B.P. Halloran, R.G. Kirk USE OF BERYLLIUM AND GRAPHITE-POLYMER SUBSTRATES TO REDUCE SPURIOUS X-RAY SIGNAL , Pages 591-613 , Barbara J. Panessa PROBLEMS AND PROGRESS IN QUANTITATIVE LOCALIZATION OF SODIUM, POTASSIUM, AND CHLORINE IN FREEZE-DRIED SECTIONS: USE OF ERYTHROCYTES AS A MODEL SYSTEM , Pages 615-633 , John McD. Tormey A STUDY OF THE MAINTENANCE OF CALCIUM AND PHOSPHORUS IN ANHYDROUS PREPARATIONS OF MINERALIZED TISSUE FOR ELECTRON OPTICAL EXAMINATION , Pages 635-651 , William J. Landis ELECTRON PROBE MEASUREMENT OF ELECTROLYTES IN RAT FOOT MUSCLE , Pages 653-664 , F. Duane Ingram, Mary Jo Ingram Index , Pages 665-672