Micro Total Analysis Systems 2002: Proceedings of the μTAS 2002 Symposium, held in Nara, Japan, 3–7 November 2002 Volume 1

The Sixth International Conference on Miniaturized Chemical and Biochemical Analysis Systems, known as /JTAS2002, will be fully dedicated to the latest scientific and technological developments in the field of miniaturized devices and systems for realizing not only chemical and biochemical analysis but also synthesis. The first /JTAS meeting was held in Enschede in 1994 with approximately 160 participants, bringing together the scientists with background in analytical and biochemistry with those with Micro Electro Mechanical Systems (MEMS) in one workshop. We are grateful to Piet Bergveld and Albert van den Berg of MESA Research Institute of the University of Twente for their great efforts to arrange this exciting first meeting. The policy of the meeting was succeeded by late Prof. Dr. Michael Widmer in the second meeting, /JTAS'96 held in Basel with 275 participants. The first two meetings were held as informal workshops. From the third workshop, /JTAS'98 (420 participants) held in Banff, the workshop had become a worldwide conference. Participants continued to increase in /JTAS2000 (about 500 participants) held in Enschede and /JTAS2001 (about 700 participants) held in Monterey. The number of submitted papers also dramatically increased in this period from 130 in 1998, 230 in 2000 to nearly 400 in 2001. From 2001, /JTAS became an annual symposium. The steering committee meeting held in Monterey, confrrmed the policy of former /JTAS that quality rather than quantity would be the key-point and that the parallel-session format throughout the 3. Front Matter....Pages i-xxxix Airborn Chemistry Levitated Protein Droplets as a Novel Analytical Tool for Nucleation Screening in Macromolecular Crystallisation....Pages 54-57 On-Line Monitoring of Airborne Chemistry in Levitated Droplets: In-Situ Synthesis and Application of SERS Active Ag-Sols for Trace Analysis by Raman Spectrosmetry....Pages 58-60 Chip-to-World Interfaces for High-Throughput Lab-on-a-Chip Devices....Pages 61-63 Electrodeless Dielectrophoretic Trapping and Separation of Cells....Pages 25-27 SiO 2 Nozzle Array-Based Patch-Clamp Microsystem....Pages 28-30 A Programmable Cell Assay Platform for Kinetic Studies of a Single Cell....Pages 31-33 An Integrated Microdialysis-Based System....Pages 34-36 User-Assembly, Fully Integrated Micro Chemical Laboratory Using Biochemical IC Chips for Wearable/Implantable Applications....Pages 37-39 Microfluidic Reactor Array for Multistep Droplet Reactions....Pages 40-42 Single Molecular Detection of DNA/Protein and Its Application to Biochips....Pages 43-44 Exploring FET Concepts for Lab-on-a-Chip....Pages 1-6 Monitoring of Cell Growth, Oxygen and pH in Microfermentors....Pages 7-9 Observation of Response to Stimuli in Oscillating Cells Patterned by Microfabricated Structure....Pages 10-12 On-Chip Agarose Microchamber (AMC) Array Cell-Cultivation System for Topographical Control of Neural Network....Pages 13-15 DNA Separation in Gradient of Confined Nanospace Generated by Nanoparticles in a Microchannel....Pages 45-47 Self-Assembled Magnetic Colloids for DNA Separations in Microfluidic Devices....Pages 48-50 A Microfabricated Device for Separating ∼200 Kilo-Base-Pair DNA Molecules in ∼15 Seconds....Pages 51-53 Integrated Size Exclusion and Reversed-Phase Electrochromatography....Pages 16-18 Dispersion Analysis of a Solid-Phase Microextraction Chip by Computational Fluid Dynamics....Pages 19-21 Interfacing Microchip CE with ICPMS for Element Speciation....Pages 22-24 The Mems Modeling System by Collaboration of Multi-Scale Simulators and Application to the Microreactor....Pages 64-66 FEM study of Coulter Counter with Water-Based Adaptable Aperture....Pages 67-69 Micromanipulating Magnetic Particles in Microfluidic Systems....Pages 70-72 Optimal Design of the Micromixer using Nonhomogeneous Multilayer Laminar Flow....Pages 73-75 Computational Simulations of Fluid Flow Dynamics, and Bead Packing in Solid Phase Extraction Microsystems....Pages 76-78 The Low-Voltage Cascade EOF PUMP: Comparing Theory with Published Data....Pages 79-81 Universal Joule Heating Model in Electrophoretic Separation Microchips....Pages 82-84 Mathematical Model of Interfacial Layer in Ultra-Fine Liquid Drop Based on Molecular Dynamics Simulation....Pages 85-87 Development of Micromixer Based on Instability of Interface Between Two Immiscible Liquids....Pages 88-90 Theoretical Study of AC Electroosmotic Flows in Non-Uniformly Charged Microchannels....Pages 91-93 Numerical Simulation of Mixing in a Micro-Channel with Non-Uniform Zeta Potential Surface....Pages 94-96 Injection of Sample Bands from open Channels Into Packed Separation Columns....Pages 97-99 Microscale Measurements of Flow Bounded by Air-Water Interfaces....Pages 100-102 Virtual Flow Channel: A Novel Micro-fluidics System with Orthogonal, Dynamic Control of Sample Flow Dimensions....Pages 103-105 Microfabrication of 3D Oblique Structures by Inclined UV Lithography....Pages 106-108 A New Fabrication Technique of a DNA Electrophoresischip by Silicon Micromachining Technology....Pages 109-111 Precision Patterning of PDMS Thin Films: A New Fabrication Method and Its Applications....Pages 112-114 Fabrication of Microfluidic Channels with Symmetric Cross-Sections for Integrated NMR Analysis....Pages 115-117 Precise and Feasible Fabrication Process of Microfluidic Devices on a Borosilicate Glass Chip....Pages 118-120 Laminated Electrodes Chip for Pulse-Immunoassay....Pages 121-123 Fabrication of on-Chip Sorter Devices with Sub-Micrometer Scale Channels and Self-Aligned Microelectrodes....Pages 124-126 Powder Handling Device for Drug Formulation....Pages 127-129 Thermal Micropump Using Surface Tension Imbalance in Microchannels....Pages 130-132 Optimizatiion of Electrokinetic Pumps for Chip-Based Chromatographic Separations....Pages 133-135 Practical Valves and Pumps for Large-Scale Integration into Microfluidic Analysis Devices....Pages 136-138 Electro Osmotic Flow Pump Based on the Corbino Disc Geometry....Pages 139-141 A Temperature Controlled Micro Valve for Biomedical Applications Using a Temperature Sensitive Hydrogel....Pages 142-144 Electroosmotically Driven Two-Liquid Viscous Pump for Nonconducting Liquids....Pages 145-147 BI-Directional Valve-Less Micropump Using Piezoelectric Micro-Array Actuators....Pages 148-150 Gravity-Driven Micropump with a Steady Flow Rate....Pages 151-153 A micro pump, compatible with multiple manufacturing methods, for fluidhandling in disposable microsystems....Pages 154-156 Microfabrication Process for High-density Micro Pipette Array and Matching Multi-Well Plate with Mixers....Pages 157-159 Microfabricated Phase Change Latched Valve for Integrated Devices....Pages 160-162 Thermal-Actuated Paraffin Microvalves....Pages 163-165 Injection Modes for Evaporation Driven Continuous Liquid Flow....Pages 166-168 DNA Amplification and Hybridization Assays in Integrated Plastic Monolithic Devices....Pages 169-171 Microfluidic Device for Nucleic Acid Fragmentation by Shear Force....Pages 172-174 Combined Nucleic Acid Extraction and Enrichment in Bead-Packed Plastic Beds....Pages 175-177 Measuring Lithium in Whole Blood Using Capillary Electrophoresis....Pages 178-180 An Ingestible Electronic Pill for Real Time Analytical Measurements of the Gastro-Intestinal Tract....Pages 181-183 Detection of Multiple Real-Time Nasba in Nanoliter Volume....Pages 184-186 Disposable Biochip Cartridge for Clinical Diagnostics Toward Point-of-Care Systems....Pages 187-189 Microchip-Based Enzyme-Linked Immunosorbent Assay (ELISA) System....Pages 190-192 Infrared-Mediated Thermocycling for DNA Amplification and Electrophoretic Separation on an Integrated Microchip Device....Pages 193-194 Integrated Microdevice for DNA Extraction from a Single Hair....Pages 195-197 Monolithic Sol-gel Microchip Device for Efficient Isolation of Nucleic Acid From Clinical Samples....Pages 198-200 Miniaturization of a Hematology Analyzer for Monitoring Oncology Outpatients....Pages 201-203 Fabrications and Applications of Micro Hemocytometer for Early Detection of Cancer....Pages 204-206 Enabling Technologies for a Personal Flow Cytometer, Part II: Integrated Analysis Cartridges....Pages 207-209 High Throughput Stress Marker Assay using Polymer Microchip Electrophoresis with Laser induced fluorescence Detection....Pages 210-211 DNA Size Separation Employing Micro-Fabricated Monolithic Nano-Structure....Pages 212-214 Integrated Microsystem of Isothermal Amplification of DNA and Electrophoresis on a Microfabricated Plastic Chip for Detection of Specific Gene and Analysis of Genetic Materials....Pages 215-217 SNP Analysis by Allele-Specific Extension of Fluorescently Labeled Nucleotides in a Microfluidic Flow-Through Device....Pages 218-220 Device for Label-Free Bio-Ligand Interaction Studies Based on Time Resolved Fourier Transform Infrared Spectrometry....Pages 221-223 A Disposable Passive Microfluidic System Integrated with Micromixer and DNA Purification Chip for DNA Sample Preparation....Pages 224-226 DNA hybridization feature on “Bead-array” — DNA probes on beads arrayed in a capillary....Pages 227-229 Development of a Label-Free Protein Array Chip....Pages 230-232 Detection of DNA Point Mutations in a Polymer Microfluidic Network Using a Temporal Thermal Gradient....Pages 233-235 Micro Multiple Immunoassay Using Silicon-Based Microfabricated Protein Biochip....Pages 236-238 An Active Mixer for Microscale Purification and Sequencing Reaction Clean-Up....Pages 239-241 In Vitro Protein Synthesis on a High-Integrated Microchamber Chip with Low DNA Molecules....Pages 242-244 Parallel Sequencing of Real-World Samples Using A Microfabricated Hybrid Device....Pages 245-247 Micomachinded Device for Manipulation of Charged Bio-Molecules....Pages 248-250 High Precision Micropreparative Separation System Based on Plastc Microfluidic Module — Capillary Coupling....Pages 251-253 High Fidelity and Low Cost Detection of Multi-Color Fluorescence from Biological Cells in a Micro Integrated Flow Cytometer (MIFC) with Disposable Observation Cell....Pages 254-256 Integrated Amorphous Silicon Photodiode Detector for Microfabricated Capillary Electrophoresis Devices....Pages 257-259 Integration of polymer waveguides for optical detection in biochemical microsystems....Pages 260-262 Real-Time SPR Imaging of Biochemical Reactions in Microfluidic Systems....Pages 263-265 A Miniaturized Glow Discharge Optical Emission Detector for Aqueous Analytes....Pages 266-268 Fiber-Optic Microdevices for Surface Plasmon Resonance Applied to Microfluidic Devices....Pages 269-271 Optical Recovery of Particles on a Chip Toward Cell Sorting and Bead-Bed Detection....Pages 272-274 Integrated On-Chip Absorption and Luminescence Spectroscopy with Polydimethylsiloxane (PDMS) Microfluidic Flow Channels Using Broadband Illumination....Pages 275-277 Development of Liquid/Liquid Optical Waveguide Using a Two Phase Sheath Flow and Its Application to Fluorescent Determination of Rhodamine B....Pages 278-280 Integration of Photo-Thermal Spectroscopy Detection System on a Chip....Pages 281-283 Integrated Microfluidic Optical Systems ( i MOS) with Led....Pages 284-286 Application of Organic Light Emitting Diode as an On-Chip Light Source for Fluorescence Spectroscopy....Pages 287-289 Micro-Optical Laser Induced Fluorescence Detection on a Miniaturised Flow Cytometry Device....Pages 290-292 Comparison of Different Strategies of Chemiluminescence Detection for Microchip System Fabricated in Poly(Dimethylsiloxane)....Pages 293-295 Fiber-Loop Ring-Down Spectroscopy for Enhanced Detection of Absorption with Limited Path Length....Pages 296-298 Multicomponent Analysis by Submillimeter-Effective-Length Capillary Electrophoresis using Patterned Fluorescence Correlation Spectroscopy....Pages 299-301 Photopolymerized and Photografted Porous Polymer Monoliths for Fabrication of Microfluidic Analytical Systems....Pages 332-334 Engineered Dielectric Microspheres for Use in Microsystems....Pages 335-337 Microvalve Architectures for High-Pressure Hydraulic and Electrokinetic Fluid Control in Microchips....Pages 338-340 Supramolecular and Macromolecular Approaches to Functional Nano Materials....Pages 302-304 Gas-Liquid Flows in Microchemical Systems....Pages 353-355 On-Chip Concentration of Liquid Samples Using an Air-Liquid Two-Phase Flow....Pages 356-358 Two-Phase Flows (Oil/Water): Flow Regimes and Wetting....Pages 359-361 Label-Free Dielectric Detection of DNA Hybridization with Nanogap Junctions....Pages 305-307 Detection of Single Nucleotide Incoporation Using Pyrosequencing in a Microfluidic Device....Pages 308-310 Fast Screening of Single-Nucleotide Polymorphisms Using Chip-Based Temperature Gradient Capillary Electrophoresis....Pages 311-313 Multiplexed Protein Preparation Systems for Proteomics....Pages 344-346 A Novel Miniaturized Protein Preconcentrator Based on Electric Field-Addressable Retention and Release....Pages 347-349 Design Principles, Performance and Perspectives of a Complete Miniaturized Electrophoretic Instrument....Pages 350-352 Molecular Transport through Nanometer Confined Channels....Pages 314-316 DNA Trap-and-Release Element Employing Electric and Hydro Drag Force Fields for On-Chip Pre-Treatment....Pages 317-319 Microchip Eletrophoresis Integrated with Nanofluidic Sample Handling....Pages 320-322 BioPOEMS and Nanogap DNA Junctions....Pages 341-343 Magnetophoretic-Dielectrophoretic Field-Flow Fractionation....Pages 323-325 Free-Flow Magnetophoresis — Separation of Magnetic Microparticles and Agglomerates on Chip....Pages 326-328 Hybrid Type On-Chip Magnetic Particle Separators for Accurate Positioning Magnetic Beads....Pages 329-331 Preparation of Picoliter-Sized Reaction / Analysis Chambers for Droplet-Based Chemical and Biochemical Systems....Pages 362-364 A Micro-Reactor Equipped with Sheath-Flow Injectors for High-Speed Extraction....Pages 365-367 Mass-Production System of Nearly Monodisperse Diameter Gel Particles Using Droplets Formation in a Microchannel....Pages 368-370 A Multiphase Laminar Flow Diffusion Chip with Ion Selective Electrode Detection....Pages 371-373 Micro PIV Measurement of Electroosmotic Flow....Pages 374-376 High-Efficiency Separation in Microfluidic Devices for High-Throughput Screening of Kinases....Pages 377-379 A Multi-Stage Micro-Tangential Flow Filtration Device for μ TAS Applications....Pages 380-382 Microcontact Switching of Single Molecules....Pages 383-385 Simultaneous Hydrodynamic and Electrokinetic Flow Control....Pages 386-388 A Micro-Fluidic Platform for Contactless, Dropletless Dispensing of High-Density Protein Micro-Arrays....Pages 389-391 PDMS Microstructures Integrated with Detection Elements....Pages 392-394 Development of Plastic Microneedles for Transdermal Interfacing Using Injection Molding Techniques....Pages 395-397 A Novel Bonding Technique for Micro Polymer Chip Using Sacrificial Channel and Adhesive Printing....Pages 398-400 Adhesive and Dead Volume Free Interfacing between PDMS Microfluidic Channels....Pages 401-403 High Pressure Thermal Bonding for Sealing of Plastic Microstructures....Pages 404-406 Innovative Laser Machining and Surface Modification for Plastic Microfluidic Chip....Pages 407-409 Liquid Phase 3D Channel Networks....Pages 410-412 Design and Fabrication of Metal Electrodes Implanted PDMS Structures for Micro Flow Device....Pages 413-415 Strong Bonding of Metal Thin-Film Micropatterns on PDMS Plate Using Self-Assembled Monolayers....Pages 416-418 Study on Protein (IgG) Adsorption in Terms of Surface Modification of Cyclic Olefin Copolymer (COC) for Protein Biochip....Pages 419-421 3-D Microfluidic Networks for Combinatorial Chemistry....Pages 422-424 Effect of Surface Modification on Thermoplastic Fusion Bonding for 3-D Microfluidics....Pages 425-427 Surface Modification of Microgels with N -Alkyl Fatty Acid Layers: Ion Gradient Properties....Pages 428-430 Surface Modification of PDMS for Control of Electroosmotic Flow: Characterization Using Atomic and Chemical Force Microscopy....Pages 431-433 Molecularly-Imprinted Stationary Phases for Plastic Microchip-Based Capillary Electrochomatography....Pages 434-436 Continuous Self Arrangement of Nanoparticles in a Micro-Capillary....Pages 437-439 Surface Modification of Micromolds by Fluorocarbon Films....Pages 440-442 Functionalized Parylene Coatings for Microfluidic Applications....Pages 443-445 Photoresist-Free Micropatterning of Polymer Surfaces Used in Microanalytical Devices....Pages 446-448 A Novel In-Device Enzyme Immobilization Method for Biomems, Demonstrated for a Continuous Glucose Monitor....Pages 449-451 Designing Surface Chemistries for a High Density Chemical Microarray....Pages 452-454 Micro Peptide Array Synthesis on a Chip....Pages 455-457 The Integration of Microarray and Microchannel Using Plastic Chip....Pages 458-460 Diagnostic Antigens and Antibodies Patterned by Micro Stamping System....Pages 461-463 SU-8 Structures for Integrated High-Speed Screening....Pages 464-466 Multimode Optical Waveguide Spectrscopy of Aqueous Gold Sol....Pages 467-469 Prism-Free Broadband Coupling Approach for Spectroelectrochemical Characterization of Surface-Immobilized Molecules....Pages 470-472 Smart, Temperature-Responsive Surfaces....Pages 473-475 Electrokinetic Trapping of Proteins in Cast-to-Shape Matrices....Pages 476-478 Lipid Membrane Microarray with Discrete Chambers....Pages 479-481 Multichannel Capillary Electrochromatography PMMA Microdevice with Integrated Pulsed Conductivity Detector....Pages 485-487 Development of Electrochemical Methods for Monitoring of Single Macromolecule Processes....Pages 488-490 Separation and detection of organic acids in a CE microchip with contactless four-electrode conductivity detection....Pages 491-493 Bioanalytical Devices for Highly Selective Measurement of in Vivo Biochemicals....Pages 494-496 A Novel Contactless Impedance Detector With Shallow Insulation Gap....Pages 497-499 Protein Patterning with Programmable Surface Chemistry Chips....Pages 482-484 Micromachined Polymeric Tip as An Electrospray Ionization Source....Pages 500-502 Modified Porous Silicon Surfaces as DIOS-MS Sample Plates....Pages 503-505 PDMS Electrospray Devices Fabricated by PDMS-Diamond-Coated SU-8 Masters....Pages 506-508 Microfluidic Device with Integrated Protein Digestion, Peptide Separation and Nanospray Interface on Poly (Dimethylsiloxane) PDMS Substrate....Pages 509-511 Free Flow Electrophoresis Device Showing Sub-second Isoelectric Focusing for a Peptide....Pages 539-541 Field Testing and New Applications of Gas Phase Miniature Chemical Analysis Systems (μChemlabTM)....Pages 521-523 Results from μChemLabTM/CB, a Portable System for Detecting Chemical and Biological Warfare Agents....Pages 524-526 Development of Micro Detector for Benzo[a]pyrene Monitoring....Pages 527-529 Development and Evaluation of a Gas Absorption Microchip Using Porous Glass Plate....Pages 530-532 Development and Optimization of Air-Pollutant Monitoring Device Incorporating with Microchip....Pages 533-535 Portable System for Selective Detection of Aromatic VOC Mixture-Gases Using a Microfluidic Device....Pages 536-538 Quantitative Measurement and Control of Electrokinetically Driven Flow in Microspace....Pages 512-514 Controlling Cell Development by Microfluidic Techniques: A Step Towards Whole-Cell Biosensors with Defined Biological Features....Pages 515-517 Protein Dynamics Study Using a Continuous-Flow Microfluidic Mixer....Pages 518-520 A New Application by Microfabricated Free Flow Electrophoresis Module....Pages 542-544 Nano-Fabricated Size Exclusion Chromatograph....Pages 545-547 Pinpoint Injection and Dielectrophoretic Floating of Autonomously Aligned Microtools....Pages 548-550 Rapid Precolumn Reaction and Chiral Separation on an Integrated Microchip....Pages 551-553 Stepwise Gradient of Linear Polymer Matrices in Microchip Electrophoresis for High Resolution Separation of DNA....Pages 554-556 Continuous Cell Preparation Using Traveling Wave Dielectrophoresis....Pages 557-559 A New Plastic CE Chip with Wide Optical Clarity Using Cyclic Olefin Copolymers (COC)....Pages 560-562 Microchip Eletrophoresis of Oligosaccharides from Glycoprotein....Pages 563-565 Diagnosis of Hepatitis C Virus (HCV) Through Affinity Chromatography using Photolabile Magnetic Beads Linked RNA Aptamer on a Chip....Pages 566-568 Continuous Cell Partitioning in Two-Phase Flow System....Pages 569-571 Gene Mutation Assay by Affinity Microchip Electrophoresis Using DNA-Polyacrylamide Conjugate....Pages 572-574 Removal of Sodium Ion and Chiral Analysis Using Crown Ether as a Chiral Selector in Microchip Electrophoresis....Pages 575-577 A Multilayer Microelectrode Array for Particle Separation by Dielectrophoresis....Pages 578-580 Continuous Free-Flow Electrophoresis Separation of Proteins by Microfabricated Chamber with Micromodule Fraction Separator....Pages 581-583 Viral Separations Using a Microfabricated Electrical Splitt System....Pages 584-586 Chip Based Single-Column TITP (Transient Isotachophoresis) Separator....Pages 587-589 Capillary Electrochromatography and Preconcentration of Neutral Compounds on PDMS Microchips....Pages 590-592 A Microconcentrator for Sensors and Chromatography....Pages 593-595 Miniaturization of Protein Separation: Isoelectric Focusing and SDS-Page....Pages 596-598 On-Line Mid-IR (Quantum Cascade Laser and FTIR Spectrometric) Detection in Capillary Based Separation Systems....Pages 599-601 Monolithic Silica Columns for Capillary HPLC....Pages 602-604 Back Matter....Pages 605-629