Micro Total Analysis Systems 2000 : Proceedings of the ΜTAS 2000 Symposium, Held in Enschede, The Netherlands, 14–18 May 2000

After earlier meetings in Enschede (NL, 1994), Basel (CH, 1996) and Banff (CDN, 1998), muTAS 2000 is the fourth international symposium on the subject of miniaturized techniques, methods, devices and systems for (bio)chemical analysis and synthesis. Initially started as a minor sub-topic in the large field of Micro System Technology (MST or MEMS), the field of muTAS is currently generally considered as one of the most important application areas of MST, which is reflected in the still rapidly growing research, development, and, above all, commercialization activities. Apart from further development and refining of the research on electrophoretic separation, electrokinetically driven flow systems, cell manipulation and analysis, miniaturized flow systems and study of microfluidics, the important new area of centrifugal microfluidics on CD devices receives broad attention. On the other hand, new innovations range from topics as exotic as photoacoustic detection in microreactors and molecular emission detection on a chip to very high-pressure microreactor devices and shear-flow driven separations. The enormous speed of the developments in this field is illustrated by the large number of new start-up companies, some of them based upon technologies that were not even published at the former meeting in Banff in 1998. All this illustrates the great excitement that continues to govern this field in which generation and analysis of (bio)chemical information using microtechnology becomes more and more entangled in what one could call micro (bio)chemical systems. This volume contains the proceedings of the fourth international symposium on Micro Total Analysis Systems (muTAS 2000), held 14-18 May 2000, at the University of Twente in Enschede, The Netherlands, and organised by the MESA + Research Institute. Cutting-edge research of all invited and contributed papers presented by the world's leading &mgr;TAS groups provide the newest state of the art of this electrifying, multidisciplinary field. Front Matter....Pages i-xix Self-Assembled Monolayers with Chemical Functions....Pages 53-57 The Design of a Continuous Flow Combinatorial Screening Micro Reactor System with On-Chip Detection....Pages 59-62 Miniature Tools for Combinatorial Chemistry....Pages 63-66 Derivatization of Plastic Microfluidic Devices with Polyelectrolyte Multilayers....Pages 67-70 Flow-Through Chip Calorimeter Based on Bisb/Sb-Thin-Film Thermopiles with a Thermopower of 64 mV/K....Pages 71-74 Optimization of the Droplet Formation in a Piezo-Electric Flow-Through Microdispenser....Pages 75-78 A Multi-Nozzle Piezoelectric Microdispenser for Improving the Dynamic Volumetric Range of Droplets....Pages 79-82 A Microfluidic-Based Nanoscope....Pages 83-86 Design Analysis and 3D Measurement of Diffusive Broadening in a Y-mixer....Pages 87-90 Negative dielectrophoresis prevents surfaces from blocking....Pages 91-94 Microflow Analysis Based on Seqential and Bead Injection....Pages 1-10 Utilization of Bead Based Reagents in Microfluidic Systems....Pages 11-14 Analytical Devices Based on Transverse Transport in Microchannels....Pages 15-18 Airborne Chemistry for Biological Micro Analysis....Pages 19-24 Single Organelle Analysis with Integrated Chip Electrophoresis and Optical Tweezers....Pages 25-28 Separation of Target Microbe in Microchip by Flow Balancing and Visually Guided Laser Tele-Manipulator....Pages 29-32 Microreaction Technology for Process Intensification and High Throughput Screening....Pages 33-40 Micromachined Fluidic Couplers....Pages 41-44 In-Channel Processing to Create Autonomous Hydrogel Microvalves....Pages 45-48 Miniaturization and Integration of Photoacoustic Detection with a Microfabricated Chemical Reactor System....Pages 49-52 A Valve-Less Diffuser Micropump for Microfluidic Analytical Systems....Pages 95-98 Biosystem for the culture and characterisation of epithelial cell tissues....Pages 99-102 Somatic Cell Counting with Silicon Apertures....Pages 103-106 Microfabricated Fluidic Devices for Cellular Assays....Pages 107-110 Microbiology On-a-Chip....Pages 111-114 Dielectrophoretic separation/isolation of rare particles/cell types form a heterogeneous suspension within a microfluidic system....Pages 115-118 Immuno-Electrophoresis of Blood Cells on Micro-Capillary Chips....Pages 119-122 Realisation of a Cell Manipulation Bio-Microsystem using Shadow mask techniques....Pages 123-126 A Combined Dielectrophoretic and Field-Flow Fractionation Microsystem for Biomedical Separation and Analysis....Pages 127-130 Integrated Silicon Microheating Elements using Silicon-on-Plastic Drop-In Functionality....Pages 131-134 A μTAS — Atomic Emission Flame Spectrometer (AES)....Pages 135-138 Thermal Management and Surface Passivation of a Miniaturized PCR Device for Traditional Chinese Medicine....Pages 139-142 μ-CE Chip Fabricated by Moving Mask Deep X-ray Lithography Technology....Pages 143-146 Fabrication of Artificial Muscle Based Valves for Controlled Drug Delivery....Pages 147-150 Technology chain for polymer μ-TAS applications....Pages 151-154 Development of Methods for On-Line Chemical Detection with Liquid-Phase Microchemical Reactors Using Conventional and Unconventional Techniques....Pages 155-158 Polymer Micro-Structures: Prototyping, Low-cost Mass Fabrication and Analytical Applications....Pages 159-162 Integration of Micro-Optical Systems for Fluorescence Detection in μ TAS Applications....Pages 163-166 Micro machined gas chromatograph based on a plasma polymerised stationary phase....Pages 167-170 A Micromachined Electron Beam Ion Source....Pages 171-174 Finding a good hydrogel composition for hydrogel based microvalves....Pages 175-178 Bonding and Etching Techniques for Precision Fabrication of Reliable Microvalves....Pages 179-182 Bi-Directional Valve-Less Micropump Fabricated Using Deep Reactive Ion Etching....Pages 183-186 Diamond Microchip Capillary Chromatography of Proteins....Pages 187-190 Microchamber Array for Immunosensor Applications....Pages 191-194 The Decade’s Search for the Killer Ap in μ-TAS....Pages 195-204 Structurally Programmable Microfluidic Systems....Pages 205-208 Design and Simulation of Particles and Biomolecules Handling Micro Flow Cells with Three-Dimensional Sheath Flow....Pages 209-212 Microfabricated Intrachannel Electrical Contacts for Material Transport Control....Pages 213-216 Microfabrication Technology for Chemical and Biochemical Microprocessors....Pages 217-220 High Efficiency Separations on Microchip Devices....Pages 221-224 An Integrated Fritless Column for Planar Capillary Electrochromatography with Conventional Stationary Phases....Pages 225-228 Hand-Held Miniature Chemical Analysis System (μChemlab) for Detection of Trace Concentrations of Gas Phase Analytes....Pages 229-232 Commercializing Lab-on-a-Chip Technology....Pages 233-238 Centrifugal Microfluidics: Applications....Pages 239-242 Versatile, Adaptable and Programmable Microfluidic Platforms for DNA Diagnostics and Drug Discovery Assays....Pages 243-248 Integrated Cell Based Assays in Microfabricated Disposable CD Devices....Pages 249-252 Microvolume Assays with Confocal Detection Systems....Pages 253-260 Living Cells in Opto-Electrical Cages Characterisation Manipulation and Force Measurements....Pages 261-264 Microfabricated Multi-Frequency Particle Impedance Characterization System....Pages 265-268 Novel Separation Method on a Chip Using Capillary Electrophoresis in Combination with Dielectrophoresis....Pages 269-272 Flow Profiling in Microstructures with Micrometer Resolution....Pages 273-276 An Active Silicon Micromixer for μTAS Applications....Pages 277-282 The Effect of Particles on Performance of Fixed-Valve Micropumps....Pages 283-286 Designing Corner Compensation for Electrophoresis in Compact Geometries....Pages 287-290 Computer Simulations of Electrokinetic Sample Manipulations in Microfluidic Devices....Pages 291-294 Transport Number Mismatch Induced Stacking of Swept Sample Zones for Microchip-Based Sample Concentration....Pages 295-298 Passive Microfluidics — Ultra-Low-Cost Plastic Disposable Lab-on-a-Chips....Pages 299-302 Dispersion Studies of Pressure-Driven Flow in Deep-Reactive-Ion-Etched Microfluidic Channels....Pages 303-306 Combination of Sample Pre-Concentration and Capillary Electrophoresis On-Chip....Pages 307-310 Microfluidics in a Rotating CD....Pages 311-314 Defect-Oriented Testing of Microelectronic/Fluidic Systems....Pages 315-318 A Microfluidic Sedimentation Particulate Capture Device with Internal Degassing Membranes....Pages 319-322 Self-Aligning Microfluidic Interconnects with Low Dead Volume....Pages 323-326 Development and Characterization of a Generic Microfluidic Subsystem toward Portable Biochemical Detection....Pages 327-330 TiO 2 Surface Modifications for Light Modulated Control of Flow Velocity....Pages 331-334 Polymer Microfluidic Valves, Membranes and Coatings....Pages 335-338 Novel Designs for Electrokinetic Injection in μTAS....Pages 339-342 Non-Destructive Mixing, Concentration, Fractionation and Separation of μm-sized Particles in Liquid by Ultrasound....Pages 343-346 A Closed Loop Controlled Electrochemically Actuated Micro Dosing System for in Situ Sensor Calibration....Pages 347-350 Consequences of Opposing Electrokinetically and Pressure-Induced Flows in Microchannels of Varying Geometries....Pages 351-354 On-Chip Separation of Explosive Compounds — Divided Reservoirs to Improve Reproducibility and Minimize Buffer Depletion....Pages 355-358 Counter Current Electrophoretic Sample Preparation for Microscale Chemical Analysis....Pages 359-362 Luminol Chemiluminescence Systems for Metal Analysis by μTAS....Pages 363-366 Miniaturized Capillary Isoelectric Focusing (cIEF): Towards a Portable High-Speed Separation Method....Pages 367-370 Liquid/Liquid Extraction Systems Using Micro-Contactor Arrays....Pages 371-374 A Cyclic Capillary Electrophoresis Separator on Silicon Substrate with Synchronized-Switching....Pages 375-378 Microchip-Nano-Electrospray Device for Rapid On-Chip Digestion and Mass Spectrometric Analysis of Hemoglobin Variants....Pages 379-382 Capillary Electrophoresis with Direct and Contactless Conductivity Detection on a Polymer Microchip....Pages 383-386 Interfacing Protein and Peptide Separation to Maldi-TOF MS Using Microdispensing and on-Target Enrichment Strategies....Pages 387-390 Miniaturized Capillary Electrophoresis System with Integrated Conductivity Detector....Pages 391-394 Microfabricated CE Chips with Optical Slit for UV Absorption Detection....Pages 395-398 Blood and Protein Separations Using a Micromachined Electrical Field-Flow Fractionation (μ-EFFF) System....Pages 399-402 Glucose Measurement in Blood Serum Injected by Electroosmosis into Phospholipid Polymer Coated Microcapillary....Pages 403-406 Valveless, Sealed Microfluidic Device for Automated Heterogeneous Immunoassay: Design and Operational Considerations....Pages 407-410 On-Chip Packed Channel Separations....Pages 411-414 Novel Injection Schemes for Ultra-High Speed DNA Separations....Pages 415-418 Realization of a Prototype Industrial on-Line Sensing System for Ammonium Based on Micro System Technology: Results and Future Perspectives....Pages 419-422 An Integrated System for Gene Detection Using Cycling Probe Technology....Pages 423-426 Single Molecule Detection and Bead Trapping in Microstructures for Molecule Selection and DNA Degradation....Pages 427-430 Quantitative Design and Analysis of Single-Particle Dielectrophoretic Traps....Pages 431-434 Zona Pellucida Removal of Mammalian Embryos in a Microfluidic System....Pages 435-438 Molecular Processing of a Single DNA Inside Channels for Genome Analysis....Pages 439-442 New Micro Devices for Single Cell Analysis, Cell Sorting and Cloning-on-a-Chip: The Cytocon TM Instrument....Pages 443-446 Synthesis and Analysis of Chemical Components in Nanoscale....Pages 447-454 A Microsystem Platform Interfacing MALDI-TOF MS for High Speed Automated Protein Identification....Pages 455-458 High-Density Reagent Storage Arrays for High-Throughput Screening....Pages 459-462 Towards Organic Synthesis in Microfluidic Devices: Multicomponent Reactions for the Construction of Compound Libraries....Pages 463-465 Nanochip Technology for Single DNA Molecule Analysis and Human Genomic Polymorphism Analysis....Pages 467-472 Micromachined Flow-through Filter-Chamber for Solid Phase DNA Analysis....Pages 473-476 Parallel DNA Sequencing on Microfabricated Electrophoresis Chips....Pages 477-480 Formation and Active Mixing of Metered Nano/Picoliter Liquid Droplets in a Microfluidic Device....Pages 481-484 High Throughput Screening/Residue Analysis and High Speed Protein Digestion Using Miniaturized Membrane Reactors/Separator....Pages 485-488 High-Throughput Screening on Microchips....Pages 489-492 Multi Micro Reactors Consist of Individually Temperature Controlled Silicon Well Arrays Realizing Efficient Biochemical Reactions....Pages 493-496 Plastic Microchip Electrophoresis for Clinical Applications of DNA Analysis....Pages 497-500 DNA Preparation by using a DNA Chip....Pages 501-504 Thermal Gradient DNA Chip....Pages 505-508 Enzyme-Based Electrochemical Biosensor with DNA Array Chip....Pages 509-512 Integration of Immunosorbent Assay System into a Multichannel Microchip for Clinical Diagnoses....Pages 513-515 A Microbead Sieve and Polarographic Detection Cell for Immunoassays with a Modular Microfluidic Interconnect....Pages 517-520 High-Throughput SNP Scoring in a Disposable Microfabricated CD Device....Pages 521-524 Real-time PCR Analysis on Nucleic Acids Purified from Plasma Using a Silicon Chip....Pages 525-528 Examination of Dielectrophoretic Behavior of DNA as a Function of Frequency from 30 Hz to 1 MHz Using a Flexible Microfluidic Test Apparatus....Pages 529-532 Multichannel Microchip System for Rapid Calibration and Immunoassay....Pages 533-536 mRNA Isolation for cDNA Library Construction on a Chip....Pages 537-540 Sub-Microliter Assays and DNA Analysis on Plastic Microfluidics....Pages 541-544 A Miniaturized and Integrated Plastic Thermal Chemical Reactor for Genetic Analysis....Pages 545-548 Nanofabricated Device for Fluorescence Correlation Spectroscopy in Sub-Femtoliter Volumes....Pages 549-552 Protein Microarrays for Monitoring of Structural Changes of Proteins Via Surface Enhanced Metal NANO Cluster Resonance....Pages 553-556 Miniaturized Electrospraying as a Technique for the Production of Microarrays of Reproducible Micrometer Sized Protein Spots....Pages 557-560 Computational Simulation of Bio-Microfluidic Processes in Integrated DNA Biochips....Pages 561-564 A Centrifugal Microfluidic Platform — A Comparison....Pages 565-570 An AC Magnetohydrodynamic Microfluidic Switch....Pages 571-574 Hydrophobic Valves by Ink-Jet Printing on Plastic CDs with Integrated Microfluidics....Pages 575-578 Design Analyses of Capillary Burst Valves in Centrifugal Microfluidics....Pages 579-582 Electrokinetic Pump Application in Micro-Total Analysis Systems Mechanical Actuation to HPLC....Pages 583-590 An Atmospheric Pressure Plasma on a Chip Applied as a Molecular Emission Detector in Gas Chromatography....Pages 591-594 A Micro Viscosity Detector for a Planar Hydrodynamic Chromatography (HDC) System....Pages 595-598 Shear-driven Chromatography: The Route Towards Pressure Drop-Less Chromatographic Separations in Nanometric Channels....Pages 599-602 Shah Convolution Fourier Transform Detection....Pages 603-606 Macromolecular Crystallization Microarrayed Chip for Super Saturated Vapor and Liquid-Liquid Diffusion Screening....Pages 607-610 High Pressure Gas-Liquid Mixtures Generated in a Micro-Electrolysis Cell....Pages 611-614 Back Matter....Pages 615-623