روش‌های تحلیل آنزیمی. [جلد ۱

Methods of Enzymatic Analysis focuses on the general progress in enzymology and in the special field of enzymatic analysis. This book explores the commercial production of biochemical reagents for analysis and explains the transition from the possible use of enzymatic analysis to its various applications in pure and applied biochemistry. Organized into four sections, this book starts with an overview of the basis of enzymatic analysis and provides general experimental guidelines for the techniques of measurement and for the disintegration of cells and tissues. This text then provides detailed instructions for the determination of substrates and assay of enzyme activities. Other chapters explore the practical aspects and information necessary for the application of reagents to enzymatic analysis, including sources, stability, and purity required. The final section describes the commercially available enzymes, coenzymes, substrates, and several less common reagents. Biochemists, biophysicists, researchers, and graduate students will find this book extremely useful. Content: Front Matter , Page iii Copyright , Page iv Foreword , Page v , Theodor Bücher Preface to the First Edition , Page vii , Hans-Ulrich Bergmeyer Editor's Preface to the Second Printing , Page viii , Hans-Ulrich Bergmeyer Contributors , Pages xvi-xx Abbreviations , Pages xxi-xxiii Principles of Enzymatic Analysis , Pages 3-13 , Hans-Ulrich Bergmeyer Experimental Techniques , Pages 14-42 , Hans-Ulrich Bergmeyer Cell and Tissue Disintegration , Pages 43-55 , Benno Hess Preliminary Remarks , Page 58 , Hans-Ulrich Bergmeyer Glycogen: Determination as d -Glucose with Hexokinase, Pyruvic Kinase and Lactic Dehydrogenase , Pages 59-62 , Gerhard Pfleiderer Starch , Page 63 , William J. Whelan Cellulose , Pages 64-71 , Geoffrey Halliwell Hemicelluloses , Pages 72-78 , Geoffrey Halliwell Heparin: Determination with Ribonuclease , Pages 79-86 , Nepomuk Zöllner, Bruno Lorenz Hyaluronic Acid , Pages 87-92 , Helmut Greiling Raffinose , Pages 93-98 , H.C. Siegfried de Whalley Sucrose , Pages 99-102 , Hans-Ulrich Bergmeyer, Helmut Klotzsch Lactose , Pages 103-106 , Francis J. Reithel d -Sedoheptulose-7-phosphate , Pages 107-110 , Efraim Racker d -Sedoheptulose-1,7-diphosphate: Determination with Aldolase , Pages 111-116 , Bernard L. Horecker d -Glucose: Determination with Hexokinase and Glucose-6-phosphate Dehydrogenase , Pages 117-130 , Milton W. Slein d -Glucose-1-phosphate , Pages 131-133 , Hans-Ulrich Bergmeyer, Helmut Klotzsch d -Glucose-6-phosphate and d -Fructose-6-phosphate: Determination with Glucose-6-phosphate Dehydrogenase and Phosphoglucose Isomerase , Pages 134-138 , Hans-Jürgen Hohorst d -Gluconate , Pages 139-142 , Irwin G. Leder d -6-Phosphogluconate , Pages 143-145 , Hans-Jürgen Hohorst d -Glucosamine , Pages 146-150 , David H. Brown d -Glucosamine-6-phosphate , Pages 151-155 , David H. Brown d -Fructose , Pages 156-159 , Helmut Klotzsch, Hans-Ulrich Bergmeyer d -Fructose-1,6-diphosphate: Determination with Fructose-1,6-diphosphatase , Pages 160-163 , Efraim Racker l -Sorbose-6-phosphate , Pages 164-166 , Efraim Racker d -Sorbitol , Pages 167-170 , H. Guy Williams-Ashman myo-Inositol , Pages 171-174 , Arthur Weissbach d -Ribose-5-phosphate , Pages 175-177 , Efraim Racker l -Ribulose and l -Arabinose , Pages 178-181 , Bernard L. Horecker d -Ribulose , Pages 182-185 , Robert C. Nordlie, Herbert J. Fromm d -Ribulose-5-phosphate , Pages 186-187 , Efraim Racker d -RibuIose-1,5-diphosphate , Pages 188-190 , Efraim Racker l -Xylulose , Pages 191-193 , Gilbert Ashwell d -Xylulose: Determination with DPN-xylitol Dehydrogenase , Pages 194-195 , Gilbert Ashwell d -Xylulose and d -Xylose: Determination with d -Xylose Isomerase , Pages 196-200 , Bernard L. Horecker d -Xylulose-5-phosphate , Pages 201-204 , Efraim Racker d -Erythrose-4-phosphate , Pages 205-207 , Efraim Racker l -Erythrulose , Pages 208-210 , Helmut Holzer, Heinz Werner Goedde Glycerol , Pages 211-214 , Otto Wieland L -(—)-Glycerol-1-phosphate: Determination with Glycerol-l-phosphate Dehydrogenase , Pages 215-219 , Hans-Jürgen Hohorst d -GIycerate , Pages 220-223 , Reinhard Kattermann, August Holldorf, Helmut Holzer d -3-Phosphoglycerate, d -2-Phosphoglycerate, Phosphoenolpyruvate , Pages 224-233 , Rudolf Czok, Lieselotte Eckert d -1,3-Diphosphoglycerate , Pages 234-237 , Erwin Negelein d -2,3-Diphosphoglycerate , Pages 238-240 , Isidore Krimsky l -Glyceraldehyde-3-phosphate , Pages 241-243 , Efraim Racker Dihydroxyacetone , Pages 244-245 , Otto Wieland Dihydroxyacetone Phosphate, Fructose-1,6-diphosphate and d -Glyceraldehyde-3-phosphate: Determination with Glycerol-l-phosphate Dehydrogenase, Aldolase and Triosephosphate Isomerase , Pages 246-252 , Theodor Bücher, Hans-Jürgen Hohorst Pyruvate , Pages 253-259 , Theodor Bücher, Rudolf Czok, Walther Lamprecht, Erwin Latzko Hydroxypyruvate , Pages 260-262 , Helmut Holzer, August Holldorf β-Mercaptopyruvate , Pages 263-265 , Ernest Kun l -(+)-Lactate: Determination with Lactic Dehydrogenase and DPN , Pages 266-277 , Hans-Jürgen Hohorst d -Lactate , Pages 278-282 , C.J.A. v.d. Hamer Methylglyoxal , Pages 283-284 , Helmut Klotzsch, Hans-Ulrich Bergmeyer Ethanol: Determination with Alcohol Dehydrogenase and DPN , Pages 285-289 , Roger Bonnichsen Acetaldehyde: Determination with Alcohol Dehydrogenase from Yeast , Pages 290-296 , Hans-Ulrich Bergmeyer Glycolaldehyde , Pages 297-299 , Helmut Holzer, Heinz Werner Goedde Glyoxylate , Pages 300-302 , Helmut Klotzsch, Hans-Ulrich Bergmeyer Acetate , Pages 303-307 , Frank Lundquist Formate , Pages 308-312 , Jesse C. Rabinowitz, William E. Pricer Jr. Citrate: Determination with Citrase , Pages 313-317 , Stanley Dagley Citrate and Isocitrate: Determination with Aconitase and Isocitric Dehydrogenase , Pages 318-323 , Günther Siebert α-Oxoglutarate , Pages 324-327 , Hans-Ulrich Bergmeyer, Erich Bernt l -(—)-Malate: Determination with Malic Dehydrogenase and DPN , Pages 328-334 , Hans-Jürgen Hohorst Oxaloacetate , Pages 335-339 , Hans-Jürgen Hohorst, Martin Reim Succinate , Pages 340-345 , Thomas P. Singer, Paul Bernath, Carol J. Lusty Fumarate , Pages 346-349 , Thomas P. Singer, Carol J. Lusty Characterization of Peptides and Proteins with Enzymes , Pages 350-362 , Hugo Fasold, Gerd Gundlach Glutathione , Pages 363-366 , Helmut Klotzsch, Hans-Ulrich Bergmeyer d -Amino Acids , Pages 367-372 , Paul Boulanger, Roger Osteux l -Amino Acids: l -Lysine, l -Arginine, l -Ornithine, l -Tyrosine, l -Histidine, l -Glutamic Acid, l -Aspartic Acid , Pages 373-377 , Ernest F. Gale l -Alanine: Determination with Glutamate-Pyruvate Transaminase and Lactic Dehydrogenase , Pages 378-380 , Gerhard Pfleiderer l -Aspartic Acid and l -Asparagine: Determination with Glutamate-Oxaloacetate Transaminase and Malic Dehydrogenase , Pages 381-383 , Gerhard Pfleiderer l -Glutamate: Determination with Glutamic Dehydrogenase , Pages 384-397 , Erich Bernt, Hans-Ulrich Bergmeyer dl -Threonine , Pages 398-400 , Martin Flavin Urea , Pages 401-406 , Erich Bernt, Hans-Ulrich Bergmeyer Creatine , Pages 407-410 , Erich Bernt, Hans-Ulrich Bergmeyer, Hans Möllering Polyunsaturated Fatty Acids , Pages 411-414 , Joseph MacGee Lecithin , Pages 415-418 , Hans Möllering, Hans-Ulrich Bergmeyer Acetyl Coenzyme A , Pages 419-424 , Karl Decker Acetoacetyl Coenzyme A , Pages 425-428 , Karl Decker Acrylyl Coenzyme A , Pages 429-430 , P. Roy Vagelos Benzoyl Coenzyme A , Pages 431-432 , Gerhard Michal, Hans-Ulrich Bergmeyer Butyryl-CoA and the CoA Derivatives of the Higher Saturated Fatty Acids , Pages 433-436 , Werner Seubert Crotonyl Coenzyme A , Pages 437-440 , Karl Decker l -(+)-β-Hydroxybutyryl Coenzyme A , Pages 441-444 , Karl Decker β-Hydroxy-β-methylglutaryl Coenzyme A , Pages 445-448 , Joachim Knappe β-Hydroxypropionyl Coenzyme A , Pages 449-451 , P. Roy Vagelos Malonylsemialdehyde Coenzyme A , Pages 452-453 , P. Roy Vagelos Acetoacetate , Pages 454-458 , Jane Mellanby, Dermot H. Williamson d -(—)-β-Hydroxybutyrate , Pages 459-461 , Dermot H. Williamson, Jane Mellanby Hydrolysis of Steroid Conjugates , Pages 462-476 , Klaus-Dieter Voigt 20-Ketosteroids , Pages 477-484 Steroid Alcohols in Urine , Pages 485-490 , Hans Joachim Hübener Adenosine , Pages 491-494 , Hans Möllering, Hans-Ulrich Bergmeyer Hypoxanthine and Xanthine , Pages 495-499 , Søren Jørgensen Uric Acid , Pages 500-501 , Elith Praetorius Inosine , Pages 502-504 , Alan Coddington Guanosine , Pages 505-507 , Alan Coddington Orotic acid , Pages 508-511 , Herbert C. Friedmann, Gladys Krakow Coenzyme A , Pages 512-527 , Gerhard Michal, Hans-Ulrich Bergmeyer Diphosphopyridine Nucleotide (DPN) , Pages 528-530 , Martin Klingenberg Reduced Diphosphopyridine Nucleotide (DPNH) , Pages 531-534 , Martin Klingenberg Triphosphopyridine Nucleotide (TPN) , Pages 535-536 , Martin Klingenberg Reduced Triphosphopyridine Nucleotide (TPNH) , Pages 537-538 , Martin Klingenberg Adenosine-5′-triphosphate: Determination with Phosphoglycerate Kinase , Pages 539-558 , Hans Adam Adenosine-5′-triphosphate and Creatine Phosphate: Determination with Luciferase , Pages 559-572 , Bernard L. Strehler Adenosine-5′-diphosphate and Adenosine-5′-monophosphate , Pages 573-577 , Hans Adam Adenosine Phosphates , Pages 578-580 , Hans Möllering, Hans-Ulrich Bergmeyer Uridine Diphosphoglucose, Uridine Diphosphogalactose, Uridine Triphosphate and Uridine Diphosphoglucuronic Acid , Pages 581-595 , George T. Mills, Evelyn E.B. Smith Flavine Adenine Dinucleotide , Pages 596-598 , Herbert C. Friedmann Flavine Mononucleotide , Pages 599-601 , Herbert C. Friedmann Thiamine Pyrophosphate , Pages 602-605 , Erika Holzer, Hans-Dieter Söling, Heinz Werner Goedde, Helmut Holzer Pyridoxal-5-phosphate and Pyridoxamine-5-phosphate , Pages 606-609 , Gerhard Schreiber, Helmut Holzer Creatine Phosphate , Pages 610-616 , Walther Lamprecht, Philipp Stein Organophosphorous Insecticides , Pages 617-625 , Paul A. Giang Di-(p-chlorophenyl)trichlorethane (DDT) , Pages 626-632 , Herbert Keller Inorganic Peroxides , Pages 633-635 , Erich Bernt, Hans-Ulrich Bergmeyer Nitrate , Pages 636-639 , Fujio Egami, Shigehiko Taniguchi Magnesium in Plasma , Pages 640-643 , R. Czok Pyrophosphate , Pages 644-647 , Kenneth Bailey Preliminary Remarks , Page 650 , Hans-Ulrich Bergmeyer The Importance of the Measurement of Enzyme Activity in Medicine , Pages 651-678,678a,678b,678c,678d,679-712 , E. Schmidt, F.W. Schmidt, H.D. Horn, U. Gerlach The Importance of the Measurement of Enzyme Activity in Food Chemistry , Pages 713-719 , Josef Schormüller The Importance of the Measurement of Enzyme Activity in Botanical and Agricultural Chemistry , Pages 720-723 , Eduard Hofmann Fructose-1,6-diphosphate Aldolase , Pages 724-731 , Friedrich H. Bruns, Hans-Ulrich Bergmeyer 1-Phosphofructoaldolase , Pages 732-735 , Hans-Peter Wolf Lactic Dehydrogenase , Pages 736-743 , Hans-Ulrich Bergmeyer, Erich Bernt, Benno Hess Glucose-6-phosphate Dehydrogenase: (Zwischenferment) , Pages 744-751 , Georg Wilhelm Löhr, Hans Dierck Waller Glutamic Dehydrogenase , Pages 752-756 , Ellen Schmidt Malic Dehydrogenase , Pages 757-760 , Hans-Ulrich Bergmeyer, Erich Bernt Sorbitol Dehydrogenase , Pages 761-764 , Ulrich Gerlach Acetylcholinesterase , Pages 765-770 , Wolfgang Pilz Cholinesterases , Pages 771-775 , Hans Bockendahl, Robert Ammon Lipase , Pages 776-778 , Arnold M. Seligman, Stanley P. Kramer Phosphatases (Phosphomonoesterases) , Pages 779-787 , Kurt Linhardt, Klaus Walter Glucose-6-phosphatase , Pages 788-792 , Alfred E. Harper Ribonuclease , Pages 793-799 , Nepomuk Zöllner, Gerd Hobom Chymotrypsin , Pages 800-806 , Wirnt Rick Trypsin , Pages 807-818 , Wirnt Rick Pepsin, Pepsinogen, Uropepsinogen , Pages 819-823 , Wirnt Rick Peptidases , Pages 824-836 , Heinz Wüst Glutamate-Oxaloacetate Transaminase , Pages 837-845 , Hans-Ulrich Bergmeyer, Erich Bernt Glutamate-Pyruvate Transaminase , Pages 846-853 , Hans-Ulrich Bergmeyer, Erich Bernt Amylase , Pages 854-858 , Harold V. Street Creatine Phosphokinase , Pages 859-862 , Erich Bernt, Hans-Ulrich Bergmeyer Galactose-1-phosphate Uridyl Transferase , Pages 863-866 , Kurt J. Isselbacher β-Glucosidase , Pages 867-868 , Eduard Hofmann β-Glucuronidase , Pages 869-874 , William H. Fishman Glutathione Reductase , Pages 875-879 , Hans-Dieter Horn Kallikrein , Pages 880-884 , Ivar Trautschold, Eugen Werle Catalase , Pages 885-894 , Hans Lück Peroxidase , Pages 895-897 , Hans Lück Reductases , Pages 898-900 , Hans Lück Invertase , Pages 901-907 Thromboplastin Time (Prothrombin Time) , Pages 908-912 , Heinrich Südhof Urease , Pages 913-916 , Eduard Hofmann Xanthine Oxidase , Pages 917-919 , Hans Lück Histochemical Detection of Enzymes , Pages 920-963 , Franz Duspiva Biochemical Reagents , Pages 967-1037 , Hans-Ulrich Bergmeyer, Helmut Klotzsch, Hans Möllering, Michael Nelböck-Hochstetter, Klaus Beaucamp Conversion Table: Transmittance ⇌ Extinction , Pages 1041-1044 Atomic Weights , Page 1045 Index , Pages 1047-1064