Methods in Protein Sequence Analysis : Proceedings of the 7th International Conference, Berlin, July 3–8, 1988

"Methods in Protein Sequence Analysis - 1988" - contains selected contributions on modern protein- analytical techniques as presented by speakers at the Seventh International Conference on "Methods in Protein Sequence Analysis", held from July 3rd to July 8th, 1988 in Berlin. The book contains information on new methodologies for sensitive amino acid analysis, N- and C-terminal sequence analysis, and protein and peptide purification. In addition recent mass spectrometric approaches are described, as an alter­ native technique to the common stepwise degradative sequence analysis of polypeptides by the Edman method. The book presents new possibilities in the design of sequencers and sophisticated equipment for the structural analysis of peptides and proteins. It describes practical approaches for the investigation of protein domains and protein complexes, and contains review chapters on the crystallization of cell organelles as well as on recent theoretical aspects of protein folding mechanisms. The nature of protein folding is not yet understood, but further advances in this area would greatly enhance our present knowledge of protein structure and function. Further, the book gives examples of the application of gene technology to protein characterization and to the design of new proteins. This enables new studies on the structure and function of proteins to be made, and opens up efficient approaches to the design of drugs. Front Matter....Pages I-XXXV Front Matter....Pages 1-1 Detection Limits in Amino Acid Analysis: An Overview....Pages 2-9 Amino Acid Analysis at High Sensitivity: A Critical Review of the Communications....Pages 10-13 A Comparison of 9-Fluorenylmethylchloroformate (FMOC-Cl) Amino Acid Analysis with Ninhydrin and Phenylthiocarbamyl (PTC) Chemistries....Pages 14-18 Localization of Endogenous Phosphoserine Residues in the Primary Structure of Proteins....Pages 19-22 Quantitative N-terminal Analysis of Polypeptides....Pages 23-32 Front Matter....Pages 33-33 Study on New Edman-type Reagents....Pages 34-41 Protein Microsequence Analysis with Dansylamino-PITC....Pages 42-51 Manual Gas Phase Isothiocyanate Degradation....Pages 52-59 Front Matter....Pages 60-60 A Second Generation Solid-phase Protein Sequencer: The ProsequencerTM....Pages 61-68 Development of Membrane Supports for the Solid-phase Sequence Analysis of Proteins and Peptides....Pages 69-78 Accelerated High Sensitivity Sequence Analysis of Proteins and Peptides Immobilized on Chemically-modified Glass Fiber Discs....Pages 79-97 A New Modular Sequencer....Pages 98-107 An Improved Injection System for On-line High Sensitivity Phenylthiohydantoin Amino Acid Analysis....Pages 108-111 Design and Performance Features of a Simplified Protein Sequencer with an On-Board Isocratic HPLC System....Pages 112-115 New Aspects in Isocratic HPLC Separation of Phenylthiohydantoin Amino Acids Through the Application of Ionic Detergents....Pages 116-121 A Procedure for the Manual Precyclization of Fiber Glass Filters used in the ABI 470A Protein Sequencer....Pages 122-127 Front Matter....Pages 128-128 The Long Search for a Viable Method of C-terminal Sequencing....Pages 129-136 C-terminal Sequencing: A New Look at the Schlack-Kumpf Thiocyanate Degradation Procedure....Pages 137-144 Carboxyl-terminal Sequence Analysis of Proteins and Peptides by Chemical Methods....Pages 145-151 Primary Structure Determination of Proteins by Using a Set of Directly Purified C-terminal Peptides....Pages 152-155 Front Matter....Pages 128-128 Immobilized Anhydrotrypsin as a Specific Affinity Adsorbent for Polypeptides Containing Arginyl, Lysyl, or S-Aminoethylcysteinyl Residues at the C-termini....Pages 156-163 Obtaining Primary Sequence Information from the Carboxy Terminal End of Polypeptides....Pages 164-167 Reinvestigation on BNPS-Skatole Cleavage of Tryptophan Polypeptides and the C-Terminal Sequencing of the Cleavage Fragments....Pages 168-173 Front Matter....Pages 174-174 Advances in Peptide and Protein Sequencing by High Performance Tandem Mass Spectrometry....Pages 175-182 Protein Sequence Analysis by Tandem Quadrupole Fourier Transform Mass Spectrometry....Pages 183-190 Strategy for the Use of Plasma Desorption Mass Spectrometry in Protein Sequence Analysis....Pages 191-198 Peptide Analysis by Time-of-flight Secondary Ion Mass Spectrometry....Pages 199-205 Analysis of Post-translational Modification and Processing by High Mass FAB Mass Spectrometry....Pages 206-218 Front Matter....Pages 219-219 NH 2 -Terminal and Internal Microsequencing of Proteins Electroblotted on Inert Membranes....Pages 220-233 Initial and Repetitive Yields from Proteins Blotted on PVDF Membranes....Pages 234-239 Evaluation of Siliconized Glass Fiber Membrane for Protein Chemical Analysis....Pages 240-247 Rapid Peptide Mapping and Protein Analysis by HPLC....Pages 248-255 Application of Microbore HPLC for Purifying Proteins and Peptides for Microsequence Analysis....Pages 256-263 Effect of Bead Size on the Analysis of Proteins and Peptides by High Pressure Liquid Chromatography....Pages 264-272 The Influence of SDS on Micropreparative Peptide Separations on Reversed Phase Columns....Pages 273-279 Micro-manipulation and Micro-sequencing of Proteins....Pages 280-283 Preparative Separation of Bioactive Protein on High Performance Hydrophobic Interaction Chromatography....Pages 284-288 Cuttelfish Protamines: Amino-acid Sequences of Three Distinct Variants....Pages 289-292 Direct Characterization of Proteins and Peptides in HPLC by Photodiode Array UV-VIS Detection: A New Approach in the Detection and Characterization of Polypeptides....Pages 293-300 Front Matter....Pages 301-301 A Fluorescent Labelling Procedure to Facilitate Protein Isolation for Rapid Structural Analysis on a Microscale: Application to Analysis of Post-translational Processing of Newcastle Disease Virus Proteins....Pages 302-309 Front Matter....Pages 301-301 Specific Enzymatic Cleavage at Cystine/Cysteine Residues. The Use of ASP-N Endoproteinase....Pages 310-314 Limited Proteolysis as a Tool to Detect Structure and Dynamic Features of Globular Proteins: Studies on Thermolysin....Pages 315-324 Biochemical Characterization of Posttranslational Processing Enzymes....Pages 325-328 Characterization of Gonadotropin Hormone-Releasing Hormone Analogs....Pages 329-336 Brain Nicotinic Acetylcholine Receptors: A Gene Family....Pages 337-351 N α -Acetylation of Eukaryotic Proteins: Purification and Characterization of Yeast N α -Acetyltransferase and Acetylcoenzyme A Hydrolase....Pages 352-355 Front Matter....Pages 356-356 International Cooperation Among Protein Sequence Data Banks....Pages 357-360 Current Status of Protein Data Banks....Pages 361-364 Database Lysis: Computer-assisted Investigation of Cleavage Sites in Proteins....Pages 365-368 Front Matter....Pages 369-369 Preferred Residue Interactions in Protein Subunit and Domain Interfaces....Pages 370-378 Protein Structural Elements and Folding....Pages 379-384 Membrane-controlled Folding Mechanism of Hepatitis B Surface Antigen of Human, Woodchuck, Ground Squirrel and Duck....Pages 385-388 The Three-dimensional Structure of the Pore-forming Fragment of Colicin A....Pages 389-396 Structural Studies on Crystals of Ribosomal Particles....Pages 397-405 Front Matter....Pages 406-406 Cystatins and Cystatin-like Domains of Multifunctional Proteins: Solid-phase Microsequencing as a Tool to Study Structure-Function Relationships of Protein Inhibitors....Pages 407-414 New Inhibitory Properties of Eglin C after Specific Mutagenesis....Pages 415-422 Immuno-chemical Recognition of Synthetic Peptides Based on the Sequence and Three-dimensional Structure of Human Renin: An Immuno-control of Renin Activity....Pages 423-430 Sequence Directed Design of Recognition Peptides....Pages 431-438 Front Matter....Pages 439-439 The Domain Structures of the E2 Components of the Pyruvate Dehydrogenase Multienzyme Complexes from Bacillus stearothermophilus and Saccharomyces cerevisiae ....Pages 440-449 The Identification and Significance of Substructural Domains....Pages 450-457 Front Matter....Pages 439-439 Identification of Murine Nuclear Proteins that Bind to the Octamer Control Sequence of Immunoglobulin Genes using 5’-Amino-Oligonucleotides....Pages 458-465 Structure and Functional Domains of Human Apolipoprotein B-100: A Strategy to Elucidate the Structure Information of a Large Protein....Pages 466-474 Front Matter....Pages 475-475 Synthesis of Immunotoxins Using a Temperature Controlled Cross-linking Agent....Pages 476-479 The Extracellular Functional Sites on the α-Subunit of Acetylcholine Receptor and of the Receptor-Binding Sites on α-Neurotoxin....Pages 480-491 The Antigenic Structure of Lysozyme as Detected by T-cells....Pages 492-500 Front Matter....Pages 501-501 Proteinchemical Methods in Use with Complex Membrane Enzymes (Cytochrome C Oxidase)....Pages 502-508 Rhodopsin and G-Protein Linked Receptors....Pages 509-516 The Cholinergic Binding Site: From Sequence to Function....Pages 517-521 Characterization of Proteins Involved in Mammalian Mitosis....Pages 522-529 Front Matter....Pages 530-530 Protein Chemistry Renascent....Pages 531-535 Protein Chemistry and the Biotechnology of the Future....Pages 536-553 Back Matter....Pages 554-575 "Methods in Protein Sequence Analysis - 1988"--Contains selected contributions on modern protein- analytical techniques as presented by speakers at the Seventh International Conference on "Methods in Protein Sequence Analysis", held from July 3rd to July 8th, 1988 in Berlin. The book contains information on new methodologies for sensitive amino acid analysis, N- and C-terminal sequence analysis, and protein and peptide purification. In addition recent mass spectrometric approaches are described, as an alterƯ native technique to the common stepwise degradative sequence analysis of polypeptides by the Edman method. The book presents new possibilities in the design of sequencers and sophisticated equipment for the structural analysis of peptides and proteins. It describes practical approaches for the investigation of protein domains and protein complexes, and contains review chapters on the crystallization of cell organelles as well as on recent theoretical aspects of protein folding mechanisms. The nature of protein folding is not yet understood, but further advances in this area would greatly enhance our present knowledge of protein structure and function. Further, the book gives examples of the application of gene technology to protein characterization and to the design of new proteins. This enables new studies on the structure and function of proteins to be made, and opens up efficient approaches to the design of drugs The most recent methods and strategies for the elucidation of protein and peptide structures, e.g. sensitive techniques for the analysis of primary structure and amino acid sequences as well as secondary and tertiary structures are discussed in detail. They include sophisticated crystallization procedures for proteins and cell organelles, folding mechanisms of proteins, strategies for domain structure and binding site evaluation and advanced immunological methods. Analyses of structure-function correlations important for genetic engineering and the design of new drugs or proteins are also reported. A special chapter addresses the documentation of protein sequences, the organization of protein data bases and their use in the search and comparison of proteins.