Methods in Enzymolgoy, Volume 295: Energetics of Biological Macromolecules, Part B (Methods in Enzymology)

**General Description of the Volume:**The very existence of biological structures and their functional interactions are dictated by energetic relationships. Thus the central theme of this volume is that thermodynamic methods, i.e. techniques that probe the energetics of biological macromolecules, now comprise a powerful and practical family of tools for research in modern biology. The application of thermodynamics and statistical thermodynamics to biochemical and biophysical systems is presented. This volume supplements **Methods in Enzymology, Volume 259**.**General Description of the Series:**The critically acclaimed laboratory standard for more than forty years, **Methods in Enzymology** is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features\* Major topics covered include:\* Deciphering rules of helix stability in peptides\* Protein Folding in Membranes\* Molecular Crowding\* Study of the Bohr Effect in Hemoglobin Intermediates\* Photoacoustic Calorimetry of Proteins\* Theoretical Aspects of Isothermal Titration Calorimetry\* Energetic Methods to Study Bifunctional Biotin Repressor Content: Contributors to volume 295 Pages ix-xi Preface Page xiii Gary K. Ackers, Michael L. Johnson Volumes in series Pages xv-xxxi [1] Deciphering rules of helix stability in peptides Original Research Article Pages 1-26 Carol A. Rohl, Robert L. Baldwin [2] Modified amino acids as probes of helix stability Original Research Article Pages 26-41 Neville R. Kallenbach, Erik J. Spek [3] Measuring hydration changes of proteins in solution: Applications of osmotic stress and structure-based calculations Original Research Article Pages 42-62 Vince J. Licata, Norma M. Allewell [4] Protein folding in membranes: Determining energetics of peptide-bilayer interactions Original Research Article Pages 62-87 Stephen H. White, William C. Wimley, Alexey S. Ladokhin, Kalina Hristova [5] Tight ligand binding affinities determined from thermodynamic linkage to temperature by titration calorimetry Original Research Article Pages 88-99 Michael L. Doyle, Preston Hensley [6] Structure-based prediction of binding affinities and molecular design of peptide ligands Original Research Article Pages 100-122,IN1-IN2,123-127 Irene Luque, Ernesto Freire [7] Molecular crowding: Analysis of effects of high concentrations of inert cosolutes on biochemical equilibria and rates in terms of volume exclusion Original Research Article Pages 127-149 Allen P. Minton [8] Application of automated methods for determination of protein conformational stability Original Research Article Pages 150-170 Jeffery M. Schwehm, Wesley E. Stites [9] Calculations of proton-binding thermodynamics in proteins Original Research Article Pages 170-189 Paul Beroza, David A. Case [10] Analysis of spectra from multiwavelength oxygen-binding studies of mixed metal hybrid hemoglobins Original Research Article Pages 190-207 Alexandra L. Klinger, Gary K. Ackers [11] Study of the Bohr effect in hemoglobin intermediates Original Research Article Pages 208-227 Louise Benazzi, Rosaria Russo, Marilena Ripamonti, Michele Perrella [12] Application of planck-benzinger relationships to biology Original Research Article Pages 227-268 Paul W. Chun [13] Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors Original Research Article Pages 268-282,IN3,283-294 Thomas A. Morton, David G. Myszka [14] Prediction of binding energetics from structure using empirical parameterization Original Research Article Pages 294-315 Brian M. Baker, Kenneth P. Murphy [15] Photoacoustic calorimetry of proteins Original Research Article Pages 316-330 Mark A. McLean, Carmelo Di Primo, Eric Deprez, Gaston Hui Bon Hoa, Stephen G. Sligar [16] Isonergonic cooperativity: A novel form of allostery Original Research Article Pages 331-349 Harvey F. Fisher, Jon Tally [17] Theoretical aspects of isothermal titration calorimetry Pages 350-364 Lawrence Indyk, Harvey F. Fisher [18] Use of poisson-boltzmann equation to analyze ion binding to DNA Original Research Article Pages 364-378 Victor A. Bloomfield, Ioulia Rouzina [19] Following the folding of RNA with time-resolved synchrotron X-ray footprinting Original Research Article Pages 379-402 Bianca Sclavi, Sarah Woodson, Michael Sullivan, Mark Chance, Michael Brenowitz [20] Analysis of interactions between CytR and CRP at CytR-regulated promoters Original Research Article Pages 403-424 Donald F. Senear, Laura T. Perini, Stacey A. Gavigan [21] Energetic methods to study bifunctional biotin operon repressor Original Research Article Pages 424-450 Dorothy Beckett [22] Thermal melting properties of C-terminal domain mutants of bacteriophage О» c I repressor Original Research Article Pages 450-467 Eddine K. Merabet, David S. Burz, Gary K. Ackers [23] Investigation of phase transitions in bilayer membranes Original Research Article Pages 468-494 Jeffrey T. Mason [24] Membrane-confined analytical electrophoresis Original Research Article Pages 494-518 Thomas M Lade, Harvey K Shepard, Theresa M Ridgeway, Thomas P Moody, Timothy J Wilson Author index Pages 519-536 Subject index Pages 537-548 General Description of the Volume: The very existence of biological structures and their functional interactions are dictated by energetic relationships. Thus the central theme of this volume is that thermodynamic methods, i.e. techniques that probe the energetics of biological macromolecules, now comprise a powerful and practical family of tools for research in modern biology. The application of thermodynamics and statistical thermodynamics to biochemical and biophysical systems is presented. This volume supplements Methods in Enzymology, Volume 259 . General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Major topics covered include: * Deciphering rules of helix stability in peptides * Protein Folding in Membranes * Molecular Crowding * Study of the Bohr Effect in Hemoglobin Intermediates * Photoacoustic Calorimetry of Proteins * Theoretical Aspects of Isothermal Titration Calorimetry * Energetic Methods to Study Bifunctional Biotin Repressor