Methods in cell biology. Volume 74, Development of sea urchins, ascidians, and other invertebrate deuterostomes : experimental approaches

This book provides a practical guide to experimental methods for studying the development invertebrate deuterostomes as animal model systems. The chapters provide detailed experimental protocols that cover a broad range of topics in modern experimental methods. Topics covered range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches. * Covers a wide range of methods, from classical embryology through modern genomics* Discusses animals related to vertebrates, providing a valuable evolutionary perspective* Includes a practical guide to the use of sea urchins in the teaching laboratory Content: Series Editor Page ii Contributors Pages xvii-xxi Preface Pages xxiii-xxiv The Invertebrate Deuterostomes: An Introduction to Their Phylogeny, Reproduction, Development, and Genomics Original Research Article Pages 1-13 Charles A. Ettensohn, Gary M. Wessel, Gregory A. Wray Care and Maintenance of Adult Echinoderms Original Research Article Pages 17-38 S.Anne Böttger, Charles W. Walker, Tatsuya Unuma Echinoderm Eggs and Embryos: Procurement and Culture Original Research Article Pages 39-74 Kathy R. Foltz, Nikki L. Adams, Linda L. Runft Culture of Echinoderm Larvae through Metamorphosis Original Research Article Pages 75-86 Gregory A. Wray, Chisato Kitazawa, Benjamin Miner Obtaining and Handling Echinoderm Oocytes Original Research Article Pages 87-114 Gary M. Wessel, Ekaterina Voronina, Jacqueline M. Brooks Procurement and Culture of Ascidian Embryos Original Research Article Pages 115-141 Billie J. Swalla Culture of Adult Ascidians and Ascidian Genetics Original Research Article Pages 143-170 Carolyn Hendrickson, Lionel Christiaen, Karine Deschet, Di Jiang, Jean-Stéphane Joly, Laurent Legendre, Yuki Nakatani, Jason Tresser, William C. Smith Hemichordate Embryos: Procurement, Culture, and Basic Methods Original Research Article Pages 171-194 Christopher J. Lowe, Kuni Tagawa, Tom Humphreys, Marc Kirschner, John Gerhart Cephalochordate (Amphioxus) Embryos: Procurement, Culture, and Basic Methods Original Research Article Pages 195-215 Linda Z. Holland, Ju-Ka Yu Quantitative Microinjection of Oocytes, Eggs, and Embryos Original Research Article Pages 219-242 Laurinda A. Jaffe, Mark Terasaki Blastomere Isolation and Transplantation Original Research Article Pages 243-271 Hyla Sweet, Shonan Amemiya, Andrew Ransick, Takuya Minokawa, David R. McClay, Athula Wikramanayake, Ritsu Kuraishi, Masato Kiyomoto, Hiroki Nishida, Jonathan Henry Isolation and Culture of Micromeres and Primary Mesenchyme Cells Original Research Article Pages 273-285 Fred H. Wilt, Stephen C. Benson Rapid Microinjection of Fertilized Eggs Original Research Article Pages 287-310 Melani S. Cheers, Charles A. Ettensohn Methods for Embryo Dissociation and Analysis of Cell Adhesion Original Research Article Pages 311-329 David R. McClay Analysis of Sea Urchin Embryo Gene Expression by Immunocytochemistry Original Research Article Pages 333-369 Judith M. Venuti, Carmen Pepicelli, Vera Lynn Flowers Light Microscopy of Echinoderm Embryos Original Research Article Pages 371-409 Laila Strickland, George von Dassow, Jan Ellenberg, Victoria Foe, Peter Lenart, David Burgess TEM and SEM Methods Original Research Article Pages 411-441 Bruce J. Crawford, Robert D. Burke Calcium Imaging Original Research Article Pages 443-468 Michael Whitaker Labeling of Cell Membranes and Compartments for Live Cell Fluorescence Microscopy Original Research Article Pages 469-489 Mark Terasaki, Laurinda A. Jaffe Isolation of Organelles and Components from Sea Urchin Eggs and Embryos Original Research Article Pages 491-522 Gary M. Wessel, Victor D. Vacquier Sea Urchin Spermatozoa Original Research Article Pages 523-544 Victor D. Vacquier, Noritaka Hirohashi Measuring Ion Fluxes in Sperm Original Research Article Pages 545-576 Alberto Darszon, Christopher D. Wood, Carmen Beltrán, Daniel Sánchez, Esmeralda Rodríguez, Julia Gorelik, Yuri E. Korchev, Takuya Nishigaki Isolating DNA, RNA, Polysomes, and Protein Original Research Article Pages 579-599 Bruce P. Brandhorst Detection of mRNA by In Situ Hybridization and RT-PCR Original Research Article Pages 601-620 Andrew Ransick Using Reporter Genes to Study cis -Regulatory Elements Original Research Article Pages 621-652 Maria I. Arnone, Ivan J. Dmochowski, Christian Gache Identification of Sequence-Specific DNA Binding Proteins Original Research Article Pages 653-675 James A. Coffman, Chiou-Hwa Yuh Expression of Exogenous mRNAs to Study Gene Function in the Sea Urchin Embryo Original Research Article Pages 677-697 Thierry Lepage, Christian Gache Disruption of Gene Function Using Antisense Morpholinos Original Research Article Pages 699-711 Lynne M. Angerer, Robert C. Angerer Generation and Use of Transgenic Ascidian Embryos Original Research Article Pages 713-730 Robert W. Zeller Genomic Resources for the Study of Sea Urchin Development Original Research Article Pages 733-757 R. Andrew Cameron, Jonathan P. Rast, C. Titus Brown Genomic Resources for Ascidians: Sequence⧸Expression Databases and Genome Projects Original Research Article Pages 759-774 Nori Satoh Gene Regulatory Network Analysis in Sea Urchin Embryos Original Research Article Pages 775-794 Paola Oliveri, Eric H. Davidson Sea Urchin Gametes in the Teaching Laboratory: Good Experiments and Good Experiences Original Research Article Pages 797-823 David Epel, Victor D. Vacquier, Margaret Peeler, Pam Miller, Chris Patton Appendix Pages 825-840 Index Pages 841-875 Volumes in Series Pages 877-883 This book provides a practical guide to experimental methods for studying the development invertebrate deuterostomes as animal model systems. The chapters provide detailed experimental protocols that cover a broad range of topics in modern experimental methods. Topics covered range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches.

* Covers a wide range of methods, from classical embryology through modern genomics
* Discusses animals related to vertebrates, providing a valuable evolutionary perspective
* Includes a practical guide to the use of sea urchins in the teaching laboratory "This book provides a practical guide to experimental methods for studying the development invertebrate deuterostomes as animal model systems. The chapters provide detailed experimental protocols that cover a broad range of topics in modern experimental methods. Topics covered range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches. Covers a wide range of methods, from classical embryology through modern genomics. Di."--[Source inconnue] Part I. Procurement, maintenance & culture of oocytes, embryos, larvae and adults Part II. Embryological approaches Part III. Cell biological approaches Part IV. Molecular biological approaches Part V. Genomics Part VI. Echinoderm eggs and embryos in the teaching lab.