Metastasis Research Protocols (Methods in Molecular Biology, Vol. 878) (Methods in Molecular Biology, 878)

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases. The second edition of Metastasis Research Protocols, brings together the most up to date versions of the seminal techniques that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Presented by top scientists, the collection includes a wide spectrum of articles encompassing important key methods and to introduce new methods which are making an impact in the area of metastasis research. Volume 1 includes key cellular and molecular techniques relevant to the exploration of cancer cells and tissues, the focus is on the tools that have been shown to be helpful in unravelling the molecular processes important in cancer metastasis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research. Basic Immunocytochemistry For Light Microscopy / Susan A. Brooks -- Lectin Histochemistry To Detect Altered Glycosylation In Cells And Tissues / Susan A. Brooks And Debbie M.s. Hall -- Histopathological Assessment Of Metastasis / Derek E. Roskell And Ian D. Buley -- Immunocytochemical Detection And Characterisation Of Individual Micrometastatic Tumour Cells / Stephan Braun And Klaus Pantel -- The Polymerase Chain Reaction / Hazel M. Welch -- Sodium Dodecyl Sulphate-polyacrylamide Denaturing Gel Electrophoresis And Western Blotting Techniques / Christine Blancher And Rob Mccormick -- 2-de-based Proteomics For The Analysis Of Metastasis-associated Proteins / Miriam Dwek And Diluka Peiris -- Assessment Of Gelatinases (mmp-2 And Mmp-9) By Gelatin Zymography / Marta Toth, Anjum Sohail, And Rafael Fridman --^ Determination Of Cell-specific Receptor Binding Using A Combination Of Immunohistochemistry And In Vitro Autoradiography : Relevance To Therapeutic Receptor Targeting In Cancer / Michael R. Dashwood And Marilena Loizidou -- Fluorescence In Situ Hybridization For Cancer-related Studies / Lyndal Kearney And Janet Shipley -- Using A Quartz Crystal Microbalance Biosensor For The Study Of Metastasis Markers On Intact Cells / Julien Saint-guirons And Björn Ingemarsson -- Cell Separations By Flow Cytometry / Derek Davies -- Detection Of Putative Cancer Stem Cells Of The Side Population Phenotype In Human Tumor Cell Cultures / Matthias Christgen ... [et Al.] -- Genes Involved In The Metastatic Cascade Of Medullary Thyroid Tumours / Caroline Schreiber, Kirsten Vormbrock And Ulrike Ziebold -- High-resolution Quantitative Methylation Analysis Of Microrna Genes Using Pyrosequencingtm / Ulrich Lehmann, Cord Albat, And Hans Kreipe --^ Rnai Technology To Block The Expression Of Molecules Relevant To Metastasis : The Cell Adhesion Molecule Ceacam1 As An Instructive Example / Daniel Wicklein -- Galectin-3 Binding And Metastasis / Pratima Nangia-makker, Vitaly Balan, And Avraham Raz -- Lectin Array-based Strategies For Identifying Metastasis-associated Changes In Glycosylation / Simon Fry ... [et Al.] Edited By Miriam Dwek, Susan A. Brooks, Udo Schumacher. Includes Bibliographical References And Index. Metastasis Research Protocols Preface Acknowledgements Contents Contributors Chapter 1: Basic Immunocytochemistry for Light Microscopy Chapter 2: Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues Chapter 3: Histopathological Assessment of Metastasis Chapter 4: Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells Chapter 5: The Polymerase Chain Reaction Chapter 6: Sodium Dodecyl Sulphate–Polyacrylamide Denaturing Gel Electrophoresis and Western Blotting Techniques Chapter 7: 2-DE-Based Proteomics for the Analysis of Metastasis-Associated Proteins Chapter 8: Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography Chapter 9: Determination of Cell-Speci fi c Receptor Binding Using a Combination of Immunohistochemistry and In Vitro Autoradiography: Relevance to Therapeutic Receptor Targeting in Cancer Chapter 10: Fluorescence In Situ Hybridization for Cancer-Related Studies Chapter 11: Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells Chapter 12: Cell Separations by Flow Cytometry Chapter 13: Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cell Cultures Chapter 14: Genes Involved in the Metastatic Cascade of Medullary Thyroid Tumours Chapter 15: High-Resolution Quantitative Methylation Analysis of MicroRNA Genes Using PyrosequencingTM Chapter 16: RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive Example Chapter 17: Galectin-3 Binding and Metastasis Chapter 18: Lectin Array-Based Strategies for Identifying Metastasis-Associated Changes in Glycosylation INDEX Now in a second, revised edition, this practical companion to the methodologies of researching cancer growth includes topical introductions and stepwise, reproducible protocols for laboratory processes, as well as invaluable troubleshooting tips.