Mass Spectrometry of Proteins and Peptides (Methods in Molecular Biology Vol 146)

John R. Chapman recreates the success of his earlier acclaimed book (Protein and Peptide Analysis by Mass Spectrometry, 1996) with a major new collection of cutting-edge methods in the analysis of proteins and peptides. Contributed by well-established investigators, each chapter provides background information before guiding the researcher step-by-step through the experimental process, with detailed instructions to help assure experimental success. The applications covered range widely and include protein sequencing, higher-order structure determination, epitope mapping, kinetics, quantitation, glycosylation analysis, and bacterial typing. Authoritative and eminently practical, Mass Spectrometry of Proteins and Peptides demonstrates the full scope of this versatile analytical technique. All protein chemists and biochemists, as well as bioanalytical scientists, who want to exploit these powerful methods in their work, will find this book indispensable. New types of mass spectrometric instrumentation and new ionization techniques have dramatically extended the applicability of mass spectrometry to the analysis of proteins and peptides. In Mass Spectrometry of Proteins and Peptides, John R. Chapman recreates the success of his earlier acclaimed book (Protein and Peptide Analysis by Mass Spectrometry, 1996) with a major new collection of cutting-edge methods in this area. Contributed by well-established investigators, each chapter provides background information before guiding the researcher step-by-step through the experimental process, with detailed instructions to help assure experimental success. The applications covered range widely and include protein sequencing, higher-order structure determination, epitope mapping, kinetics, quantitation, glycosylation analysis, and bacterial typing. Authoritative and eminently practical, Mass Spectrometry of Proteins and Peptides demonstrates the full scope of this versatile analytical technique. All protein chemists and biochemists, as well as bioanalytical scientists, who want to exploit these powerful methods in their work, will find this book indispensable. Since the publication John Chapman's highly successful book, Protein and Peptide Analysis by Mass Spectrometry, new types of spectrometric instruments have appeared so that new techniques are being developed while older techniques have become more effective. This book brings us to the present practice of protein analysis using mass spectrometry. It features the latest ionization techniques for the analysis of high molecular weight materials. Also covered are the vast improvements in the sample handling technology that allows for the analysis of much smaller sample amounts as well as more intractable samples. Molecular interaction, ion structures, quantitation and kinetics are some of the newer areas reported in this volume. All the methods are presented in a step-by-step protocol supported by a helpful notes section that guides the reader carefully though the pitfalls and nuances of the techniques The purpose of Calpain Methods and Protocols is quite straightf- ward: it is to present the actual experimental methods used in many different laboratories for the study of calpain.