Mammalian cell biotechnology in protein production /editors, Hansjörg Hauser, Roland Wagner

“Hauser and Wagner have presented the new possibilities of Mammalian Cell Biology in a very informative and stimulating manner. __Prof. Dr. Hans Fritz, Ludwig-Maximilians-University Munic__ 1 Genetic Manipulation of Mammalian Cells 1.1 Hererologous Expression of Genes in Mammalian Cells 1.2 Permanent Gene Expression in Mammalian Cells: Gene Transfer and Selection 1.3 Vectors for Gene Transfer and Expression in Animal Cells 1.4 Aspects of Gene Transfer and Gene Amplification in Recombinant Mammalian Cells 1.5 Isolation of Recombinant Cell Clones Exhibiting High-Level Expression of the Introduced Gene 1.6 Genetic Engineering of Antibodies and Derivatives from Mammalian Cells 1.7 Safety Evaluation of Products Derived From Mammalian Cell Lines 2 Biological Aspects of Animal Cells 2.1 Metabolic Control of Animal Cell Culture Processes 2.2 Glycosylation: A Post-Translational Modification 3 Cell Cultivation Technology 3.1 Bioreactors Designed for Animal Cells 3.2 Hydrodynamic Properties in Bioreactors 3.3 Kinetics and Simulation of Animal Cell Processes 3.4 On-line and Off-line Process Analysis 4 Down-Stream Processing 4.1 Principles of Product Extraction from Cell Culture and Purification for Pharmaceutical Proteins 4.2 Validation of Downstream Processes Index

“Hauser and Wagner have presented the new possibilities of Mammalian Cell Biology in a very informative and stimulating manner.”
Prof. Dr. Hans Fritz, Ludwig-Maximilians-University Munich

Main description: "Hauser und Wagner haben die neuen Möglichkeiten der Mammalian Cell Biology sehr anregend dargestellt.0Prof. Dr. Hans Fritz, Ludwig-Maximilians-Universität München „Hauser und Wagner haben die neuen Möglichkeiten der Mammalian Cell Biology sehr anregend dargestellt." Prof. Dr. Hans Fritz, Ludwig-Maximilians-Universität München This text introduces the fundamental principles and practical application of techniques used in the large scale production of proteins with mammalian cell cultures. In this article I will review some of the basic features of expression in mammalian cells that are relevant to the specific manipulation of mammalian cells.