Macromolecules in the Functioning Cell

This Volume Contains Nineteen Contributions On Some Of The Most Relevant Topics In Modern Molecular Biology And Biochemistry Presented By Leading Scientists Of The Ussr And Italy. One Group Of Papers Are Mainly Concerned With The Structure And Functions Of The Genetic Elements In Eukaryote Cells; Among The Topics Are The Following: Nucleosome Structure, Characteriza Tion Of The Nuclear Matrix, Ribosomal Gene Organization, Gene Ex Pression During The Cell Cycle, And Mapping Of The Mitochondrial Transcripts. Several Other Aspects Of Macromolecule Structure And Function Have Been Discussed: Trna Modification, Translation Fac Tors, Rna Interaction With Rna-polymerase, Dna-dependent Atpases, Proteins Involved In Active Transport, Enzyme Induction, Iron And Sulfur Proteins, Etc. Furthermore, Some Studies On Macromolecule Changes In Embryonic Development And Cell Differentiation Have Been Presented, Including Dna Methylation And Macromolecular Synthesis In Sea Urchins, Polyribosomes In Loach, And Histone Modifications In Spermiogenesis. Finally, A Stimulating And Brilliant Presenta Tion On Protein--nucleic Acid Interaction By Professor Engelhardt Closes The Scientific Contributions. The Papers Collected In This Volume Have Been Presented At The First Soviet-italian Symposium On Macromolecules In The Functioning Cell Sponsored By The Italian Society Of Biochemistry, The Ussr Academy Of Sciences, And The Capri Center For Cell Biology And Natural Sciences, And Supported Mainly By The Italian National Research Council (cnr). The Efforts Of Professor A. Ruffo, As Chairman Of The Scientific Committee, Have To Be Particularly Ack Nowledged As Being Instrumental In The Success Of The Symposium. Front Matter....Pages i-ix Front Matter....Pages 1-1 Yeast Ribosomal Genes....Pages 3-14 Characterization of the Nuclear Matrix of Rat Liver and Hepatoma 27....Pages 15-29 The Physical Map of the Various Transcripts of Rat Liver Mitochondrial DNA....Pages 31-47 Organization of Lac Repressor, RNA Polymerase and Histones of DNA....Pages 49-57 Organization of the Ribosomal Genes Cluster of the Loach....Pages 59-69 A Novel Type of Gene Organization in Eukaryotic Chromosomes....Pages 71-77 Differential Gene Expression during the Cell Life Cycle....Pages 79-108 Front Matter....Pages 109-109 Eukaryotic Translation Factors and RNA-Binding Proteins....Pages 111-129 Methylation of Transfer Ribonucleic Acid....Pages 131-147 Interaction of E.Coli RNA Polymerase with Substrates during Initiation of RNA Synthesis at Different Promoters....Pages 149-158 DNA-Dependent ATPases and Viceversa....Pages 159-185 Biosynthesis of Iron-Sulfur Structures in Iron-Sulfur Proteins....Pages 187-203 Some Properties of Two Proteins Involved in Membrane Transport....Pages 205-218 Enzyme Induction and Deinduction in Animal Cell Associations....Pages 219-235 Front Matter....Pages 237-237 Enzymatic Modifications of Nuclear DNA in the Early Embryonic Development of the Sea Urchin....Pages 239-253 Regulation of Macromolecular Synthesis during Sea Urchin Development....Pages 255-261 Nucleus-Associated Polyribosomes in Early Embryos of Loach....Pages 263-282 Nucleic Acids, Histones and Spermiogenesis: The Poly(Adenosine Diphosphate Ribose)Polymerase System....Pages 283-300 On the Principles of Nucleic Acid — Protein Interaction....Pages 301-310 Introductory Talk....Pages 311-314 Front Matter....Pages 237-237 Closing Talk....Pages 315-316 Closing Talk....Pages 317-319 Back Matter....Pages 321-341 I: Structure and Functions of the Genetic Elements.- Yeast Ribosomal Genes.- Characterization of the Nuclear Matrix of Rat Liver and Hepatoma 27.- The Physical Map of the Various Transcripts of Rat Liver Mitochondrial DNA.- Organization of lac Repressor, RNA Polymerase and Histones on DNA.- Organization of the Ribosomal Genes Cluster of the Loach.- A Novel Type of Gene Organization in Eukaryotic Chromosomes.- Differential Gene Expression During the Cell Life Cycle.- II: Macromolecule Structure and Function.- Eukaryotic Translation Factors and RNA-Binding Proteins.- Methylation of Transfer Ribo