Lentivirus Gene Engineering Protocols : Second Edition

Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. __Lentivirus Gene Engineering Protocols, Second Edition__ reflects the spectacular progress made in the field with a set of cutting-edge methods contributed by highly respected scientists. Beginning with a thorough overview of the most recent lentivirus developments, the book continues with detailed protocols including sections on the advances in lentiviral vector technology, new lentiviral vector applications, involving transgenic human embryonic stem cells and fetal gene therapy among other topics, as well as the invaluable breakthroughs in LV-mediated expression of microRNAs. Written in the highly successful __Methods in Molecular Biology__TM series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, __Lentivirus Gene Engineering Protocols, Second Edition__ covers the most relevant issues and techniques of LV-based gene engineering, thus representing a complete theoretical and practical guide for scientists still unfamiliar with LV technologies and those who simply wish to know more about this vital area of study.

Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit.

Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS.

Doody Review Services

Reviewer:Omer Iqbal, MD, FACC, FESC(Loyola University Medical Center)
Description:This collection of cutting-edge protocols contributed by experts provides a thorough overview of the most recent lentivirus developments and covers advances in lentivirus vector technology, new lentivirus vector applications involving transgenic human embryonic stem cells and fetal gene therapy, and recent advances in lentivirus-mediated expression of microRNAs. The first edition was published in 2003.
Purpose:With its coverage of the most relevant issues and techniques of lentivirus-based gene engineering, the book represents a complete theoretical and practical guide for scientists and for those who simply wish to know more about lentivirus technologies.
Audience:It is intended for scientists still unfamiliar with lentivirus technologies and for those who simply wish to know more about them. In my judgment, students, postdoctoral fellows, scientists, and others interested in this field will find this book extremely useful. The chapters are contributed by recognized experts in this field.
Features:The book is divided into four parts. Part I is an overview of the lentiviral vector-mediated gene transfer and RNA silencing technology in neuronal dysfunction and part II details advances in lentiviral vector technology. Part III covers new lentiviral vector applications with chapters on lentivirus-mediated methods to establish stable transgenic human embryonic stem cells and other techniques based on the upcoming lentivirus-based fetal gene therapy for the treatment of inherited deficiencies. Part IV covers the short RNA technologies applied by lentiviral vectors with four chapters on lentivirus-mediated expression of microRNAs. Addition of colored illustrations may have enhanced the comprehension of this important field.
Assessment:This is a complete theoretical and practical guide to understanding lentivirus technologies. Scientists unfamiliar with these technologies and others interested in knowing more about them will find this book extremely useful. Given the significant advances in this field, the second edition is justified.

Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. Lentivirus Gene Engineering Protocols, Second Edition reflects the spectacular progress made in the field with a set of cutting-edge methods contributed by highly respected scientists. Beginning with a thorough overview of the most recent lentivirus developments, the book continues with detailed protocols including sections on the advances in lentiviral vector technology, new lentiviral vector applications, involving transgenic human embryonic stem cells and fetal gene therapy among other topics, as well as the invaluable breakthroughs in LV-mediated expression of microRNAs. Written in the highly successful Methods in Molecular Biology TM series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition covers the most relevant issues and techniques of LV-based gene engineering, thus representing a complete theoretical and practical guide for scientists still unfamiliar with LV technologies and those who simply wish to know more about this vital area of study. Front Matter....Pages i-xii Front Matter....Pages 1-1 Front Matter....Pages 3-35 Front Matter....Pages 38-38 Front Matter....Pages 39-52 Back Matter....Pages 53-68 ....Pages 69-76