Laboratory techniques in biochemistry and molecular biology Vol. 8, Cell culture for biochemists

This 2nd revised edition equals the popular 1st edition in providing a clear and detailed overview of cell culture. It presents information on: characteristics of cultured cells; culture vessels; glassware preparation and sterilisation techniques; subculturing; primary cells; cell culture media; techniques; contamination; the cell cycle; cell synchronisation; use of radioactive isotopes in cell culture; cell mutants and cell hybrids; viruses; and differentiation in cell cultures. Reviews on the 1st edition: ``.. the book provides an excellent insight into the way cell culture techniques can be employed in the analytical study of cellular biology.'' - Trends in Biochemical Sciences ``It is well written in a concise, easy-to-read style which stimulates the interest of the reader....'' - Science Tools ``A useful handbook on principles and practice.'' - Immunology Today v. 1. pt. 1. Electrophoresis of proteins in polyacrylamide and starch gels, by A.H. Gordon.- pt. 2. An introduction to gel chromatography, by L. Fischer.- pt. 3. Immunochemical techniques for the identification and estimation of macromolecules, by J. Clausen. v. 2. pt. 1. Automated enzyme assays, by D.B. Roodyn.- pt. 2. Cellulosic ion exchangers, by E.A. Peterson. v. 3. pt. 1. Determination of sequences in RNA, by G.G. Brownlee.- pt. 2. Techniques of lipidology, by M. Kates. v. 4. pt. 1. Chemical modification of proteins, by A.N. Glazer, R.J. DeLange and D.S. Sigman.- pt. 2. Separation methods for nucleic acids and oligonucleotides, by H. Gould and H.R. Matthews. v. 5. pt. 1. Techniques of sample preparation of liquid scintillation counting, by B.W. Fox.- Isoelectric focusing, by P.G. Righetti and J.W. Drysdale. v. 6. pt. 1. Density gradient centrifugation, by R. Hinton and M. Dobrota.- pt. 2. An introduction to radioimmunoassay and related techniques, by T. Chard. v. 7, pt. 1. Preparation and characterisation of mammalian plasma membranes, by W.H. Evans. pt. 2. An introduction to affinity chromatography, by C.R. Lowe. v. 8. Cell culture for biochemists, by R.L.P. Adams. v. 9. Sequencing of proteins and peptides, by G. Allen. v. 10. DNA sequencing, [by] J. Hindley. v. 11. Isoeletric focusing: theory, methodology and applications, [by] P. G. Righetti. v. 12. Photogenerated reagents in biochemistry and molecular biology, [by] H. Bayley. v. 13. Monoclonal antibody technology: the production and characterization of rodent and human hybridomas, [by] A.M. Campbell. v. 14. A guidebook to lipoprotein technique, [by] G.L. Mills, P.A. Lane, and P.K. Weech. v. 15. Practice and theory of enzyme immunoassays, [by] P. Tijssen. v. 16. Glycoprotein and proteoglycan techniques, [by] J.G. Beeley. v. 17. Applications of hplc in biochemistry, [by] A. Fallon, R.F.G. Booth and L.D. Bell. v. 18. Methods of cell separation, [by] P.T. Sharpe. v. 19. Synthetic polypeptides as antigens [by] M.H.V. Van Regenmortel [et al.]. v. 20. Immobilized pH gradients: theory and methodology, by] P. G. Righetti. v. 21. Isolated hepatocytes preparation, properties and applications by M.N. Berry, A.M. Edwards [and] G.J. Barritt. v. 22. Techniques in free radical research, edited by R.H. Burdon [and] P.H. van Knippenberg. v. 23. Monoclonal antibody and immunosensor technology, edited by P.C. van der Vliet. v. 24. Hybridization with nucleic acid probes.- pt. 1. Theory and nucleic acid preparation.- pt. 2. Probe labeling and hybridization techniques. v. 25. Dry chemistry. v. 26. Analysis of RNA-protein complexes in Vitro v. 27. Ultrasensitive and rapid enzyme immunoassay / Eiji Ishikawa v. 28. Synthetic peptides as antigens / M.H.V. Van Regenmortel, S. Muller. v. 29. Cancer metastasis: in vitro and in vivo experimental approaches v. 30. Inositol phospholipid metabolism and phosphatidyl inositol kinases v. 32. Magnetic cell separation. This 2nd revised edition equals the popular 1st edition in providing a clear and detailed overview of cell culture. It presents information on:
characteristics of cultured cells; culture vessels; glassware preparation and sterilisation techniques; subculturing; primary cells; cell culture media; techniques; contamination; the cell cycle; cell synchronisation; use of radioactive isotopes in cell culture; cell mutants and cell hybrids; viruses; and differentiation in cell cultures.

Reviews on the 1st edition:

``.. the book provides an excellent insight into the way cell culture techniques can be employed in the analytical study of cellular biology.'' - Trends in Biochemical Sciences

``It is well written in a concise, easy-to-read style which stimulates the interest of the reader....'' - Science Tools

``A useful handbook on principles and practice.'' - Immunology Today

Content: Edited Page ii Edited by Page iii Copyright Page Page iv List of abbreviations Pages v-vii Acknowledgements Page ix Chapter 1 Introduction Pages 1-10 Chapter 2 Characteristics of cultured cells Pages 11-33 Chapter 3 Culture vessels Pages 35-58 Chapter 4 Subculturing Pages 59-70 Chapter 5 Cell culture media Pages 71-95 Chapter 6 Primary cells Pages 97-115 Chapter 7 Techniques Pages 117-149 Chapter 8 Glassware preparation and sterilisation techniques Pages 151-164 Chapter 9 Contamination Pages 165-185 Chapter 10 The cell cycle Pages 187-210 Chapter 11 Cell synchronisation Pages 211-238 Chapter 12 Use of radioactive isotopes in cell culture Pages 239-261 Chapter 13 Cell mutants and cell hybrids Pages 263-277 Chapter 14 Viruses Pages 279-299 Chapter 15 Differentiation in cell cultures Pages 301-309 Chapter 16 Appendices Pages 311-334 References Pages 335-353 Subject Index Pages 355-364 v. 1, pt. 1. Gordon, A. H. Electrophoresis of proteins in polyacrylamide and starch gels. pt. 2. Fischer, L. Gel filtration chromatography. pt. 3. Clausen, J. Immunochemical techniques for the identification and estimation of macromolecules. v. 3, pt. 2. Kates, M. Techniques of lipidology. v. 6. pt. 2-3. Chard, T. An introduction to radioimmunoassay and related techniques. v. 8. Adams, R.L.P. Cell culture for biochemists. v. 9. Sequencing of proteins and peptides.pppp The development of advanced methods for isolation, identification and quantification of old and new inositol lipids and inositol phosphtes from natural and synthetic systems has been a major advancing force in phosphoinositol research. The writing of this book was undertaken as an opportunity fo examine the analytical validity of the biochemical transformations that constitute the basis of the lipid signaling pathways Immunoassay procedures (isotopic and non-isotopic) have become one of the single most important techniques in diagnostic medicine. This book is designed as an introductory test for the staff of clinical research laboratories who conduct or intend to conduct such techniques, and is suitable for the clinicians who make use of such services. Offers a review of developments along with tried and tested methods for isolation, resolution and quantification of inositol phospholipids and inositol polyphosphates in both cells and tissues. This book includes detailed methodology for identification of molecular species of inositol phospholipids, including their phosphates and glycans