Laboratory Protocols in Fungal Biology : Current Methods in Fungal Biology

__Laboratory Protocols in Fungal Biology__ presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. __Laboratory Protocols in Fungal Biology__ is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the __Fungal Biology__ series: Goyal, Manoharachary / __Future Challenges in Crop Protection Against Fungal Pathogens__ Martín, García-Estrada, Zeilinger / __Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites__ Zeilinger, Martín, García-Estrada / __Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2__ van den Berg, Maruthachalam / __Genetic Transformation Systems in Fungi__ Schmoll, Dattenbock / __Gene Expression Systems in Fungi__ Dahms / __Advanced Microscopy in Mycology__ Front Matter....Pages i-xxv Safety Norms and Regulations in Handling Fungal Specimens....Pages 1-7 Methods of Cryopreservation in Fungi....Pages 9-16 Long-Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results....Pages 17-65 Fungal Specimen Collection and Processing....Pages 67-72 Chemical and Molecular Methods for Detection of Toxigenic Fungi and Their Mycotoxins from Major Food Crops....Pages 73-90 Identification Key for the Major Growth Forms of Lichenized Fungi....Pages 91-112 Microscopic Methods for Analytical Studies of Fungi....Pages 113-131 Scanning Electron Microscopy for Fungal Sample Examination....Pages 133-150 High-Resolution Imaging and Force Spectroscopy of Fungal Hyphal Cells by Atomic Force Microscopy....Pages 151-160 Use of Fourier-Transform Infrared (FTIR) Microscopy Method for Detection of Phyto-Fungal Pathogens....Pages 161-167 Diagnosis of Parasitic Fungi in the Plankton: Technique for Identifying and Counting Infective Chytrids Using Epifluorescence Microscopy....Pages 169-174 Fungal Cell Wall Analysis....Pages 175-196 Histopathological Technique for Detection of Fungal Infections in Plants....Pages 197-200 Development of Media for Growth and Enumeration of Fungi from Water....Pages 201-209 Sabouraud Agar for Fungal Growth....Pages 211-216 A Method for the Formation of Candida Biofilms in 96 Well Microtiter Plates and Its Application to Antifungal Susceptibility Testing....Pages 217-223 Screening for Compounds Exerting Antifungal Activities....Pages 225-230 Fluorescence In Situ Hybridization of Uncultured Zoosporic Fungi....Pages 231-236 Staining Techniques and Biochemical Methods for the Identification of Fungi....Pages 237-257 Protocol for the In Vivo Quantification of Superoxide Radical in Fungi....Pages 259-264 Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis....Pages 265-277 Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi....Pages 279-287 Semi-Nested PCR Approach to Amplify Large 18S rRNA Gene Fragments for PCR-DGGE Analysis of Soil Fungal Communities....Pages 289-298 Proteomic Protocols for the Study of Filamentous Fungi....Pages 299-308 Detection and Quantification of Endoprotease Activity Using a Coomassie Dye-Binding Assay....Pages 309-313 Protocol of a LightCyclerTM PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples of Neutropenic Patients....Pages 315-320 Application of Polymerase Chain Reaction and PCR-Based Methods Targeting Internal Transcribed Spacer Region for Detection and Species-Level Identification of Fungi....Pages 321-330 Real-Time PCR Assay in Fungi....Pages 331-336 Quantitative Sampling Methods for the Analysis of Fungi: Air Sampling....Pages 337-341 Transformation of Filamentous Fungi in Microtiter Plate....Pages 343-347 Molecular Fingerprinting of Fungal Communities in Soil....Pages 349-356 Development of Microsatellite Markers from Fungal DNA Based on Shotgun Pyrosequencing....Pages 357-362 Multiplex and Quantifiable Detection of Infectious Fungi Using Padlock Probes, General qPCR, and Suspension Microarray Readout....Pages 363-373 Rapid Deletion Plasmid Construction Methods for Protoplast and Agrobacterium -based Fungal Transformation Systems....Pages 375-393 Improved Transformation Method for Alternaria Brassicicola and Its Applications....Pages 395-401 Methods for High-Quality DNA Extraction from Fungi....Pages 403-406 Production of Recombinant Proteins from Pichia pastoris : Interfacing Fermentation and Immobilized Metal Ion Affinity Chromatography....Pages 407-420 Development of a Real-Time Quantitative PCR Assay for the Assessment of Uncultured Zoosporic Fungi....Pages 421-426 Nucleic and Protein Extraction Methods for Fungal Exopolysaccharide Producers....Pages 427-434 Directed Evolution of a Fungal Xylanase for Improvement of Thermal and Alkaline Stability....Pages 435-446 Genome Shuffling Protocol for the Pentose-Fermenting Yeast Scheffersomyces stipitis ....Pages 447-454 Detection and Identification of Fungal Microbial Volatile Organic Compounds by HS-SPME-GC–MS....Pages 455-465 Transformation Methods for Slow-Growing Fungi....Pages 467-473 Enzymatic Saccharification of Lignocellulosic Biomass....Pages 475-481 Protoplast Fusion Techniques in Fungi....Pages 483-488 Large-Scale Production of Lignocellulolytic Enzymes in Thermophilic Fungi....Pages 489-494 Panfungal PCR Method for Detection of Aflatoxigenic Molds....Pages 495-499 Protocols for the Quantification of dsDNA and Its Fragmentation Status in Fungi....Pages 501-504 Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes....Pages 505-517 Drug-Induced Permeabilization in Fungi....Pages 519-522 Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic and Pathogenic Fungi....Pages 523-527 Identification of Mycotoxigenic Fungi Using an Oligonucleotide Microarray....Pages 529-534 DNA Microarray-Based Detection and Identification of Fungal Specimens....Pages 535-544 Bioinformatic Protocols and the Knowledge-Base for Secretomes in Fungi....Pages 545-557 High-Throughput Functional Annotation and Data Mining of Fungal Genomes to Identify Therapeutic Targets....Pages 559-564 Application of Support Vector Machines in Fungal Genome and Proteome Annotation....Pages 565-577 Bioinformatics Tools for the Multilocus Phylogenetic Analysis of Fungi....Pages 579-592 Back Matter....Pages 593-604 Annotation Fungi and their biomolecules are today being explored in a variety of scientific endeavors. This is an invaluable source of laboratory protocols for standard and applied research applications in a range of fields such as biotechnology and microbiology Fungi and their biomolecules are today being explored in a variety of scientific endeavors. This manual is an invaluable source of laboratory protocols for standard and applied research applications in a range of fields such as biotechnology and microbiology.