روش‌های آزمایشگاهی در آنزیم‌شناسی: DNA (جلد ۵۲۹)

__Methods in Enzymology__ volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future __Methods in Enzymology__ volumes. * Indispensable tool for the researcher * Carefully written and edited by experts to contain step-by-step protocols * In this volume we have brought together a number of core protocols concentrating on DNA Content: Series Page Page ii Copyright Page iv Contributors Pages xv-xvi Miscellaneous Page xvii Preface Page xix Jon Lorsch Methods in Enzymology Pages xxi-lvi Chapter One - Explanatory Chapter: PCR Primer Design Pages 1-21 RubГ©n ГЃlvarez-FernГЎndez Chapter Two - Explanatory Chapter: How Plasmid Preparation Kits Work Pages 23-28 Laura Koontz Chapter Three - Explanatory Chapter: Introducing Exogenous DNA into Cells Pages 29-34 Laura Koontz Chapter Four - Agarose Gel Electrophoresis Pages 35-45 Laura Koontz Chapter Five - Analysis of DNA by Southern Blotting Pages 47-63 Gary Glenn, Lefkothea-Vasiliki Andreou Chapter Six - Purification of DNA Oligos by Denaturing Polyacrylamide Gel Electrophoresis (PAGE) Pages 65-83 Sara Lopez-Gomollon, Francisco Esteban Nicolas Chapter Seven - Molecular Cloning Pages 85-98 Juliane C. Lessard Chapter Eight - Rapid Creation of Stable Mammalian Cell Lines for Regulated Expression of Proteins Using the GatewayВ® Recombination Cloning Technology and Flp-In T-RExВ® Lines Pages 99-124 Jessica Spitzer, Markus Landthaler, Thomas Tuschl Chapter Nine - Restrictionless Cloning Pages 125-134 Mikkel A. Algire Chapter Ten - Isolation of Plasmid DNA from Bacteria Pages 135-142 Lefkothea-Vasiliki Andreou Chapter Eleven - Preparation of Genomic DNA from Bacteria Pages 143-151 Lefkothea-Vasiliki Andreou Chapter Twelve - Preparation of Genomic DNA from Saccharomyces cerevisiae Pages 153-160 Jessica S. Dymond Chapter Thirteen - Isolation of Genomic DNA from Mammalian Cells Pages 161-169 Cheryl M. Koh Chapter Fourteen - Sanger Dideoxy Sequencing of DNA Pages 171-184 Sarah E. Walker, Jon Lorsch Chapter Fifteen - Preparation of Fragment Libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD Platform Pages 185-200 Srinivasan Yegnasubramanian Chapter Sixteen - Explanatory Chapter: Next Generation Sequencing Pages 201-208 Srinivasan Yegnasubramanian Chapter Seventeen - Generating Mammalian Stable Cell Lines by Electroporation Pages 209-226 Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, Daniel J. Leahy Chapter Eighteen - Transient Mammalian Cell Transfection with Polyethylenimine (PEI) Pages 227-240 Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, Daniel J. Leahy Chapter Ninteen - Site-Directed Mutagenesis Pages 241-248 Julia Bachman Chapter Twenty - PCR-Based Random Mutagenesis Pages 249-258 Jessica S. Dymond Chapter Twenty One - Megaprimer Method for Mutagenesis of DNA Pages 259-269 Craig W. Vander Kooi Chapter Twenty Two - Explanatory Chapter: Troubleshooting PCR Pages 271-278 Kirstie Canene-Adams Chapter Twenty Three - Explanatory Chapter: Quantitative PCR Pages 279-289 Jessica S. Dymond Chapter Twenty Four - General PCR Pages 291-298 Kirstie Canene-Adams Chapter Twenty Five - Colony PCR Pages 299-309 Megan Bergkessel, Christine Guthrie Chapter Twenty Six - Chemical Transformation of Yeast Pages 311-320 Megan Bergkessel, Christine Guthrie Chapter Twenty Seven - Transformation of E. coli Via Electroporation Pages 321-327 Juliane C. Lessard Chapter Twenty Eight - Transformation of Chemically Competent E. coli Pages 329-336 Rachel Green, Elizabeth J. Rogers Author Index Pages 337-339 Subject Index Pages 341-348

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

• Indispensable tool for the researcher
• Carefully written and edited by experts to contain step-by-step protocols
• In this volume we have brought together a number of core protocols concentrating on DNA