Isolation and Purification of Proteins (Biotechnology and Bioprocessing Series)

Considers novelties resulting from increasing demands to improve process efficiency, predictability, and economy. Assesses new trends in the design of a compact downstream processing in protein production. Release Of Protein From Biological Host /rajni Hatti-kaul And Bo Mattiasson -- Chromatography: The High-resolution Technique For Protein Separation / Peter Kumpalume And Siddhartha Ghose -- Selectivity In Affinity Chromatography / Ipsita Roy And Munishwar Nath Gupta -- Genetic Approaches To Facilitate Protein Purification /stefan Stahl [and Others] -- Solid-liquid Separation / Ricardo A. Medronho -- Membranes For Protein Isolation And Purification / Roland Ulber, [el Al.] -- Precipitation Of Proteins: Nonspecific And Specific / Ashok Kumar, [et Al.] -- Protein Crystallization For Large-scale Bioseparation / E.k. Lee And Woo-sik Kim -- Extraction For Rapid Protein Isolation / M.t. Cunha, [et Al.] -- Adsorption As An Initial Step For The Capture Of Proteins / Robert H. Clemmitt And Howard A. Chase -- Fast Chromatography Of Proteins / Per-erik Gustavsson And Per-olof Larsson -- Novel Approaches To The Chromatography Of Proteins / Ruth Freitag -- Scale-up And Commercial Production Of Recombinant Proteins / Richard Carrillo And Gene Burton -- Process Monitoring During Protein Purification / Bo Mattiasson And Rajni Hatti-kaul -- Protein Formulation / Drew N. Kelner, Wei Wang, [et Al.] -- Regulatory Requirements, Cleaning, And Sanitization Issues In Protein Purification / Gail Sofer. Edited By Rajni Hatti-kaul, Bo Mattiasson. Includes Bibliographical References And Index. This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing. This publication details the isolation of proteins from biological materials; techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring and product formulation; and regulatory and commercial considerations in protein production The means to gain access to the product from a biological source is the primary consideration during downstream processing of proteins.