Intelligent Biotechnologies of Natural and Synthetic Biologically Active Substances: XIII Narochanskie Readings (Lecture Notes in Networks and Systems, 408)

This book contains articles on innovative biotechnologies for the production and application of biopolymers and their derivatives in various industries. The conference “Intelligent Biotechnologies of Natural and Synthetic Biologically Active Substances: XIII Narochanskie Readings” took place on the base of Belarussian State University (Republic of Belarus, Minsk) and North Caucasus Federal University (Russian Federation, Stavropol) on December 1–3, 2021. The general scope of the book is current issues of biologically active substances (BAS) recovery and preparation methods development and their application in medicine, pharmaceutics, and functional nutrition. A number of original articles are devoted to the actual problems of BAS obtaining and application. A significant part of articles is related to the production of BAS nanocomplexes. Much attention is paid to promising areas of healthy foods biotechnology development and food safety. The book intends to readership specializing in the field of searching for new sources of BAS for their practical use in the framework of innovative pharmaceuticals and functional foods development Preface Contents Main Stages of the Whey Semi-finished Product Technology Development 1 Introduction 2 Materials and Methods 3 Results 4 Discussion and Conclusion References Microfiltration Processing of Raw Materials for the Fermented Milk Product Making 1 Introduction 2 Materials and Methods 3 Results 4 Discussion and Conclusion References Development of New Types of Minced Meat Products for Preventive and Personalized Nutrition 1 Introduction 2 Materials and Methods 3 Results 4 Discussion and Conclusion References Prospects for the Use of Chitosan-Stabilized Copper Nanoparticles as an Alternative to Antibiotics in Broiler Feed 1 Introduction 2 Materials and Methods 3 Results and Discussion 4 Conclusion References Proteolysis of Bovine Whey, Milk and Colostrum with Serine Endopeptidases 1 Introduction 2 Materials and Methods 3 Results 4 Discussion 5 Conclusion References TiO2-Chitosan as Universal Composite for Waste Water Treatment from Organic Pollutants 1 Introduction 2 Materials and Methods 2.1 Modification of Powdered TiO2 2.2 Optical Spectroscopy 2.3 Scanning Electron Microscopy 2.4 Study of Photocatalytic Activity 3 Results 4 Discussion and Conclusion References Isolation and Characterization of a New Fungi Isolates of the Genus TrichodermaPers. – of Potential Producers of Biofungicides 1 Introduction 2 Materials and Methods 2.1 Preparation of TrichodermaPers. Monosporic Culture 2.2 Morphological and Molecular Identification of Fungi Isolates 2.3 Kinetic Characteristics of Growth Rate in IsolatesTrichodermaPers 2.4 Fungistatic Activity of Isolates TrichodermaPers 2.5 Toxic Effect of TrichodermaPers 2.6 Statistical Analysis 3 Results 3.1 Kinetic Parameters of Fungi Growth 3.2 Fungistatic Activity of TrichodermaPers 3.3 Toxicological Effectiveness of Studied Fungi in Vivo 4 Discussion and Conclusion References Study of the Composition and in VivoEffect of Biologically Active Concentrates from Secondary Raw Materials of Millet 1 Introduction 2 Materials and Methods 2.1 Raw Materials and Preparations Used in Research 2.2 Obtaining of the Concentrates 2.3 Methods 3 Results 4 Discussion 5 Conclusion References Isolation of Cellulase-Containing Exosomal Vesicles from Picea AbiesXylem 1 Introduction 2 Materials and Methods 2.1 Plant Materials and Sample Preparation 2.2 High Performance Liquid Chromatography (HPLC) 2.3 Protein Concentration in the Exosome-Containing Fraction 2.4 Enzymatic Activity of the Exosome-Containing Fraction 2.5 Sample Preparation for Electron Microscopy 2.6 Scanning Electron Microscopy 3 Results 4 Discussion and Conclusion References Composition of Biologically Active Substances of Flower Extracts from a Variety of AchilleaSpecies and Their Antioxidant Properties 1 Introduction 2 Materials and Methods 3 Results and Discussions 4 Conclusion References Approaches to the Determination of the Growing Location of Mossy Pine Forests Based on the Spectral Characteristics of Ecologically Dependent Wood Components 1 Introduction 2 Materials and Methods 3 Results 4 Conclusion References A Novel Laccase from Basidiomycete Fomes fomentarius VKPM F-1531 1 Introduction 2 Materials and Methods 2.1 Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Technique 2.2 Enzyme Assay and Protein Content Determination 2.3 Isolation and Purification of the Extracellular Fungal Laccase 2.4 Molecular Weight Analysis Using Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis (SDS-PAGE) Technique 3 Results 3.1 Influence of Copper Ions on Laccase Activity of F. fomentariusF-1531 3.2 Purification F. fomentarius F-1531Laccase at the Optimum Concentration of Copper in the Nutrient Medium 4 Discussion 5 Conclusions References Biocompatible Materials Based on Modified Starch and Chitosan with High Mechanical Properties 1 Introduction 2 Materials and Methods 2.1 Synthesis of Graft Copolymer PS-AA 2.2 Obtaining Film Samples 2.3 Study of Mechanical Properties 2.4 Study of Biocompatibility of Films Based on Modified PS and CTS 2.5 X-ray Phase Analysis 3 Results and Discussion 3.1 Synthesis of Grafted Copolymer PS:AA 3.2 Synthesis of Film Samples 4 Conclusion References Products of Hydrolysis of Bleached Hardwood Kraft Pulp by Carbohydrate-Active Enzymes 1 Introduction 2 Materials and Methods 2.1 Purpose and Objectives of the Study 2.2 Substrates and Enzymes 2.3 Enzymatic Hydrolysis 2.4 Determining Glucose and Reducing Sugars, Inversion 2.5 Design Cryogels 3 Results 3.1 Soluble Hydrolysis Products 3.2 Degree of Hydrolysis of Polysaccharides 3.3 Design Cryogels 4 Discussion 4.1 Soluble Products and Modified Cellulose 4.2 Possibility of Obtaining Cryogel from Biomodified Cellulose 5 Conclusion References Biologically Active Substances of Extracts from Various Wood-Decay Fungi and Their Antioxidant Activity 1 Introduction 2 Materials and Methods 3 Results and Discussions 4 Conclusion References Novel Method of Preparation Biocompatible Porous Materials Based on Modified Chitosan with High Mechanical Properties 1 Introduction 2 Materials and Methods 2.1 Preparation of Porous Samples 2.2 Preparation of Film Samples 2.3 Study of Mechanical Properties 2.4 Study of Biocompatibility of Films Based on Modified PS and CTS 2.5 Study on Microstructure 3 Results and Discussion 3.1 Preparation of Porous Samples 3.2 Properties of Porous Samples 4 Conclusion References Effect of Nanosized Forms of Iron-Containing Compounds on the Growth Characteristics of Raphanus Sativus Seeds 1 Introduction 2 Materials and Methods 3 Results and Discussion 4 Conclusion References Study of the Possibility of Application of Acoustic Spectroscopy in Dairy Products 1 Introduction 2 Materials and Methods 3 Results and Discussion 4 Conclusion References Technological and Nutritional Potential of Lentil in the Turkey Cutlets Production 1 Introduction 2 Materials and Methods 3 Results 4 Conclusion References Application of Chitosan for Fermented Dairy Products with Lactobacillus delbrueckiisubsp. BulgaricusManufacturing 1 Introduction 2 Materials and Methods 3 Results 3.1 The Effect of High-Molecular Chitosan on the Cultivation of Lactobacillus delbrueckii subsp. bulgaricus 3.2 The Influence of High-Molecular Chitosan on Fermented Dairy Product Shelf Life 3.3 The Influence of High-Molecular Chitosan on the Content of Aroma-Forming and Flavour Compounds in a Fermented Dairy Product 4 Discussion and Conclusion References The Study of the Quantitative Content of Flavonoids and Biological Activity of the Herba Thlaspi Arvense L. 1 Introduction 2 Materials and Methods 3 Results 4 Discussion and Conclusion References The Obtaining an Extract from the Herba of Monarda and Determining the Indicators of Its Quality 1 Introduction 2 Materials and Methods 3 Results 4 Discussion and Conclusion References Using the Flax Seeds and the Flax Oil in the Production of Chopped Semi-finished Chicken Meat Products in Order to Enrich Them with Polyunsaturated Fatty Acids 1 Introduction 2 Materials and Methods 3 Results 4 Conclusion References Investigation of the Possibility of Using Chondroitin Sulfate in Milk Raw Materials by the Organoleptic Method 1 Introduction 2 Materials and Methods 2.1 A Subsection Sample 3 Results 4 Discussion and Conclusion References Properties of Cyclodextrins Nanocomplexes with Peptides and Fat-Soluble Vitamins 1 Introduction 2 Materials and Methods 3 Results 3.1 Characteristics of Whey Proteins Hydrolysate 3.2 Preparation and Properties of Beta-CD Nanocomplexes with Biologically Active Substances: Peptides and Fat-Soluble Vitamins A and D3 4 Discussion and Conclusion References Investigation of the Acute, Subacute and Chronic Toxicity of Curcuminoids with Native and Hydroxypropylated β-cyclodextrin Complex Nanostructures 1 Introduction 2 Materials and Methods 3 Results 3.1 Toxicological and Hygienic Assessment of Curcuminoids Nanostructures with Beta-CD in the Tetrahymena PyriformisModel System 3.2 Toxicological and Hygienic Assessment of Curcuminoids Nanostructures with Hydroxypropylated-Beta-CD in the Tetrahymena Pyriformis Model System 3.3 Toxicological and Hygienic Assessment of Curcuminoids Nanostructures with Beta-CD and Hydroxypropylated Beta-CD in the Wistar Rat Model System 4 Discussion and Conclusion References Protein-Polysaccharide Complex for the Production of an Emulsion Product 1 Introduction 2 Materials and Methods 3 Results 4 Discussion and Conclusion References Laser Doppler Flowmetry and Tissue Oxygenation Monitoring in Assessing of the Survival Rate of an Elongated Skin Flap Under the Biomaterials Application 1 Introduction 2 Materials and Methods 2.1 Vival Model 2.2 Matrices 2.3 Skin Flap Model 2.4 Animal’s Treatment 2.5 Microcirculation and Tissue Oxygenation Registration 2.6 Statistical Analysis 3 Results 3.1 The Clinical Characteristics of Skin Flap Engraftment 3.2 Intensity of Microcirculation and Tissue Oxygenation 4 Discussion and Conclusion References Development of Raw Semi-dry Sausages Enriched with Colloidal Chelate Complexes of Essential Nutrients 1 Introduction 2 Materials and Methods 2.1 Method for the Synthesis of a Colloidal Chelate Complex of Essential Nutrients 2.2 Research Methods 2.3 Development of a Recipe for Dry-Cured Sausages 3 Results 4 Discussion and Conclusion References Analysis of the Biochemical Composition and Antioxidant Capacity of Vaccinium CorymbosumL. Leaves 1 Introduction 2 Materials and Methods 3 Results and Discussion References Cryostability of Bifidobacteria in Milk and Vegetable Mixtures 1 Introduction 2 Materials and Methods 3 Results 4 Discussion 5 Conclusion References Application of Cluster Analysis of the Genus PinusVarious Species Essential Oils Terpene Compounds for Their Taxonomic Identification 1 Introduction 2 Materials and Methods 3 Results and Discussion 3.1 Intrageneric Characteristics of the Composition of Pine Essential Oils 3.2 Characteristics of Pine Essential Oils When Introduced in Belarus 3.3 Cluster Analysis of the Pine Essential Oils Composition 4 Conclusion References Author Index