Insertional Mutagenesis Strategies in Cancer Genetics [recurso electrónico

Over the past decades, insertional mutagenesis has played an important contribution to our understanding of cancer. Initially, the action of slow transforming retroviruses was used to identify endogenous cellular oncogenes (e.g. Myc, Myb). These observations sparked a series of experiments that eventually led to the idea cancer is caused by somatically acquired mutations in endogenous oncogenes and tumor suppressor genes. Since these discoveries, insertional mutagenesis has been used to identify novel cancer genes in a variety of tumor types in animal models of cancer. More recent work has developed novel insertional mutagens, such as transposons, that have broader capabilities to model cancer in vivo. While this work has focused on developing animal models of cancer, recent gene therapy trials in human patients have shown that insertional mutagenesis can also contribute to transformation. The goal of this work is summarize the contribution that insertional mutagenesis has made to our understanding of cancer. A variety of insertional mutagens are presented that have been used to study a variety of tumor types in several model organisms. In addition, the impact of insertional mutagenesis in several gene therapy trials is discussed along with strategies to avoid such complications in future clinical trials. Front Matter....Pages i-viii Insertional Mutagenesis: A Powerful Tool in Cancer Research....Pages 1-18 Retroviral Insertional Mutagenesis in Mouse Models of Leukemia and Lymphoma....Pages 19-38 Gene Discovery by MMTV Mediated Insertional Mutagenesis....Pages 39-75 Chicken Models of Retroviral Insertional Mutagenesis....Pages 77-112 Sleeping Beauty Models of Cancer....Pages 113-130 Insertional Mutagenesis in Hematopoietic Cells: Lessons Learned from Adverse Events in Clinical Gene Therapy Trials....Pages 131-165 Bioinformatics of High-Throughput Insertional Mutagenesis....Pages 167-188 Back Matter....Pages 189-200