Infrared and Raman Spectroscopy of Biological Molecules: Proceedings of the N.A.T.O. Advanced Study Institute held at Athens, Greece, August 22-31, 1978

For this summer school in Athens, Greece, August 22-21, 1978, I took as my objective the presentation of a timely representative account of the application of infrared and Raman spectro­ scopy to biological molecules. A summer school is made up of a number of things -ideas, people, organization international collaboration and sponsorship. The exchange of ideas the student-lecturer interaction in the discussion periods and the tutorials satisfy the urgent need of all the participants to meet and discuss topics of current scientific interest. It seems therefore appropriate to publish this summer school proceedings in order to make it a lasting event and that appreciation be shown to those people and institutions that made it all possible. The summer school was held under the auspices of the Greek Ministry of Culture and Sciences under the sponsorship of the NATO Scientific Affairs Division in Brussels. In addition, support was provided by the National Hellenic Research Foundation and the Ministry of Culture and Sciences for several social and scientific functions. Front Matter....Pages i-ix Front Matter....Pages 1-1 Introduction to the Raman Spectroscopy of Biological Molecules....Pages 3-34 Historical Survey of the Infrared and Raman Spectroscopic Study of Biological Molecules....Pages 35-43 Front Matter....Pages 45-45 Classical Theory of Molecular Vibrations....Pages 47-57 Correlation of Group Frequencies with Molecular Conformation....Pages 59-67 Vibrational Spectra of Solids....Pages 69-80 Group Frequencies and the Chemical Bond....Pages 81-93 Front Matter....Pages 95-95 Vibrations of Chain Molecules....Pages 97-107 Vibrational Spectroscopy of Polypeptides and Proteins....Pages 109-124 Vibrational Spectroscopy of Carbohydrates....Pages 125-137 Conformational Analysis of Snake Toxins by Laser Raman Spectroscopy....Pages 139-152 The Raman Spectroscopy of Nucleic Acids....Pages 153-165 Appendix IV Estimation of the Distortion of the Geometry of Nucleic-Acid Bases in the Excited Electronic State from the Ultra-Violet Resonance Raman Enhancement of Certain Normal Modes.*....Pages 167-173 Appendix I 15 N Isotope Effects on the Raman Spectra of 5′IMP and 5′UMP-d 3 ....Pages 175-178 Appendix II Isotope Effects on the Intensities of the Infrared and Raman Bands....Pages 179-183 Appendix III Duschinsky Effect and Raman Scattering....Pages 185-186 Interaction of Metal Ions with Nucleic Acids....Pages 187-204 Vibrational Spectroscopy of Metal Nucleic Acid Systems....Pages 205-223 Interaction of Metal Ions with Peptides in Aqueous Medium as Studied by Infrared and Raman Spectroscopy....Pages 225-240 Applications of Raman Spectroscopy to Biomembrane Structure....Pages 241-259 Resonance Raman Spectra of Model Metallo Porphins....Pages 261-265 Front Matter....Pages 95-95 Resonance Raman Spectroscopy of Heme Proteins....Pages 267-275 Front Matter....Pages 277-277 Vibrational Spectroscopy of Hydrogen Bonded Systems....Pages 279-303 Overtones and Combination Tones: Application to the Study of Molecular Associations....Pages 305-318 Infrared Studies on Anesthetic Compounds....Pages 319-324 Front Matter....Pages 325-325 Experimental Techniques of Resonance Raman Spectroscopy Applied to Biological Molecules....Pages 327-353 Techniques of Raman Spectroscopy....Pages 355-365 Back Matter....Pages 367-372 "Because of the flow of information on biological molecules and their Raman and resonance Raman spectra, it is timely and of the utmost importance to organize meetings between spectroscopists and biologists. This volume, which contains lectures given by specialists in the field of vibrational spectroscopy, has been complied to serve as an outline of the major aspects of these techniques and their recent applications to biologically important macromolecules. The lectures presented to this NATO-ASI summer school in Athens, are divided into four main sections. The first part is an introduction and historical survey of the applications of infrared and Raman spectroscopy to biological molecules. The second division contains several lectures on the classical theory of vibrations and group frequencies. The third part consist of lectures of infrared and Raman spectroscopy of biologically important macromolecules. The last section contains an account on hydrogen bonded systems and their study by infrared and Raman techniques. The papers review background material as well as reports on original research on the Raman spectra of nucleic acids; biomembranes; polypeptides and proteins. A conformational analysis of snake toxins by laser Raman spectroscopy is also included as is the effect of metal ions in nucleic acids and peptides."- Publisher Proceedings of the NATO Advanced Study Institute, Athens, Greece, August 22-31, 1978