Immobilized enzyme technology : research and applications : [proceedings of the U.S.-Japan Cooperative Science Program seminar held in Tokyo, Japan, November 5-8, 1974

On November 5-8, 1974, a Seminar on Research and De­ velopment of Immobilized Enzymes was held in Tokyo, Japan. The seminar was part of the United States-Japan Cooperative Science Program sponsored jointly by the National Science Foundation and the Japan Society for the Promotion of Sci­ ence. The purpose of the seminar was to promote the scienti­ fic exchange of ideas and scien~ific results of a practical nature, as well as academic advances made in both countries through discussion and exchange of ideas. The areas chosen for discussion included: fundamental research in immobilized enzymes, new techniques of enzyme purification, comparative studies on immobilization techni­ ques and the relative merits, chemical engineering aspects of the technology, industrial applications and reactor de­ sign. The discussions and exchange of ideas which took place at the seminar should promote new research and development which we hope will lead to new important advances in enzyme technology. These proceedings represent the summation of the work presented and discussed at the seminar. The editors hope the reader will find them interesting and informative. Howard H. Weeta11 Shuichi Suzuki March, 1975 v Contents Immobilization of Coenzyme B6 and Several B6 Enzymes: Application to Assay or Production of SOU,I;! Amino Acids and to Structure-Function Inter­ relationship Studies of B6 1 Enzymes • • • • • • • Saburo Fukui and Sei-ichiro Ikeda Enzyme Engineering Case Study: Immobilized Lactase • • • • • 17 James R. Ford and Wayne H. Pitcher, Jr. Front Matter....Pages i-ix Immobilization of Coenzyme B 6 and Several B 6 Enzymes: Application to Assay or Production of Some Amino Acids and to Structure-Function Interrelationship Studies of B 6 Enzymes....Pages 1-16 Enzyme Engineering Case Study: Immobilized Lactase....Pages 17-35 Alteration of Enzymatic Characteristics of Bacterial Alpha-Amylase by Acylation....Pages 37-51 Glucose Isomerase Immobilized on Phenol-Formaldehyde Resin....Pages 53-67 Immobilization of Enzymes by Radiation Copolymerization of Synthetic Monomers....Pages 69-72 Activity of Enzyme Immobilized by Microencapsulation....Pages 73-84 Artificial Enzyme Membranes and Enzyme Reactors....Pages 85-98 Mass Transfer and Reaction with the Multicomponent Microcapsules....Pages 99-110 Applications of Immobilized Enzymes and Immobilized Microbial Cells for L-Amino Acid Production....Pages 111-127 Kinetics and Mass Transfer Characteristics of Glucose Isomerase Immobilized on Porous Glass....Pages 129-149 Effect of Mixing and Mass Transfer on the Glucose Isomerization by Entrapped Cells in a Vertical Plate Type Reactor....Pages 151-167 Immobilization of β-Galactosidase by Polyacrylamide Gel....Pages 169-183 Immobilization of Enzymes by Radiation Polymerization....Pages 185-197 Removal of Heavy Metal Enzyme Inhibitors....Pages 199-223 External Diffusion of Enzymes Entrapped with Calcium Arginate in Packed Beds....Pages 225-240 Cellulase Production by Thermophilic Actinomyces Strain MJØr and Characterization of the Enzyme....Pages 241-251 Electrochemical Preparation of Enzyme-Collagen Membrane and Its Application....Pages 253-267 Scale-Up Studies on Immobilized, Purified Glucoamylase, Covalently Coupled to Porous Ceramic Support....Pages 269-297 Fixed Enzymes, Application of the Study of Protein Structure and Function....Pages 299-316 Back Matter....Pages 317-321 On November 5-8, 1974, a Seminar on Research and DeƯ velopment of Immobilized Enzymes was held in Tokyo, Japan. The seminar was part of the United States-Japan Cooperative Science Program sponsored jointly by the National Science Foundation and the Japan Society for the Promotion of SciƯ ence. The purpose of the seminar was to promote the scientiƯ fic exchange of ideas and scien~ific results of a practical nature, as well as academic advances made in both countries through discussion and exchange of ideas. The areas chosen for discussion included: fundamental research in immobilized enzymes, new techniques of enzyme purification, comparative studies on immobilization techniƯ ques and the relative merits, chemical engineering aspects of the technology, industrial applications and reactor deƯ sign. The discussions and exchange of ideas which took place at the seminar should promote new research and development which we hope will lead to new important advances in enzyme technology. These proceedings represent the summation of the work presented and discussed at the seminar. The editors hope the reader will find them interesting and informative. Howard H. Weeta11 Shuichi Suzuki March, 1975 v Contents Immobilization of Coenzyme B6 and Several B6 Enzymes: Application to Assay or Production of SOU, I;! Amino Acids and to Structure-Function InterƯ relationship Studies of B6 1 Enzymes " " " " " " " Saburo Fukui and Sei-ichiro Ikeda Enzyme Engineering Case Study: Immobilized Lactase " " " " " 17 James R. Ford and Wayne H. Pitcher, Jr Immobilization of Coenzyme B6 and Several B6 Enzymes: Application to Assay or Production of Some Amino Acids and to Structure-Function Interrelationship Studies of B6 Enzymes.- Enzyme Engineering Case Study: Immobilized Lactase.- Alteration of Enzymatic Characteristics of Bacterial Alpha-Amylase by Acylation.- Glucose Isomerase Immobilized on Phenol- Formaldehyde Resin.- Immobilization of Enzymes by Radiation Copolymerization of Synthetic Monomers.- Activity of Enzyme Immobilized by Microencapsulation.- Artificial Enzyme Membranes and Enzyme Reactors.- Mass Transfer and Reaction with the Multi