Illuminating Colorectal Cancer Genomics by Next-Generation Sequencing : A Big Chapter in the Tale

This book reviews the potential of next-generation sequencing (NGS) in research on and management of colorectal cancer (CRC), a leading cause of death worldwide and one of the most biologically and clinically heterogeneous cancers. It critically discusses findings from recent large-scale studies, clinical trials and meta-analyses and offers an introduction to the management of CRC in the era of precision medicine. In CRC, dozens of driver and passenger mutations are associated with the malignant transformation of epithelial cells. Consequently, the book discusses recent advances in our understanding of the genetics of CRC as a biomarker, the advent of NGS technologies in modern genomics, and the impact of NGS technology on the management of CRC. Furthermore, it highlights the potential of NGS in the context of liquid biopsy and single-cell sequencing in CRC, as well as its role in shedding light on the link between gut microbiota, immune-checkpoint blockade and CRC. The book concludes with a chapter on the limitations and cost-effectiveness of NGS in CRC. Given its scope, the book will appeal to all those interested in learning about the potential of NGS in advancing CRC research and patient care. Front Matter ....Pages i-xv An Introduction to the Current Management of Colorectal Cancer in the Era of Personalized Oncology (Angelica Petrillo, Emanuela Ferraro, Michele Ghidini, Dario Trapani)....Pages 1-27 Colorectal Cancer Genetics: An Overview of the Actionable Building Blocks (Khalid El Bairi, Csongor Lengyel, Antonio Marra, Said Afqir)....Pages 29-71 The Arrival of Next-Generation Sequencing: An Overview of Current Technologies (Khalid El Bairi, Falak Azzam, Mariam Amrani)....Pages 73-89 Next-Generation Sequencing for Colorectal Cancer Management (Khalid El Bairi, Said Afqir)....Pages 91-110 Gut Microbiota, Next-Generation Sequencing, Immune-Checkpoint Inhibitors, and Colorectal Cancer: How Hot Is the Link? (Khalid El Bairi, Mariam Amrani, Adil Maleb)....Pages 111-145 The Revolution of Liquid Biopsy and Single-Cell Sequencing in the Management of Colorectal Cancer (Khalid El Bairi, Dario Trapani, Mariam Amrani)....Pages 147-172 Overview of Cost-Effectiveness and Limitations of Next-Generation Sequencing in Colorectal Cancer (Khalid El Bairi, Falak Azzam, Dario Trapani, Bouchra Ouled Amar Bencheikh)....Pages 173-185 An Introduction to the Current Management of Colorectal Cancer in the Era of Personalized Oncology -- Colorectal Cancer Genetics: An Overview of the Actionable Building Blocks -- The Arrival of Next-Generation Sequencing: An Overview of Current Technologies -- Next-Generation Sequencing for Colorectal Cancer Management -- Gut Microbiota, Next-Generation Sequencing, Immune Checkpoint Inhibitors and Colorectal Cancer: How Hot is the Link? -- The Revolution of Liquid Biopsy and Single-Cell Sequencing in the Management of Colorectal Cancer -- Overview of Cost-Effectiveness and Limitations of Next-Generation Sequencing in Colorectal Cancer