Identification and Quantification of Drugs, Metabolites and Metabolizing Enzymes by LC-MS: Concepts, Methods and Translational Sciences (Progress in Pharmaceutical and Biomedical Analysis)

As new techniques of transferring from liquid to gas phase and measuring masses of drug molecules and metabolites become more prevalent, so do the technical challenges of putting these techniques into proper use, as well as the task of consolidating emerging applications. Identification and Quantification of Drugs, Metabolites and Metabolizing Enzymes by LC-MS, Volume 6 fills the gap in the lack of presently available literature by providing a critical review in the current use of liquid chromatography-mass spectrometry (LC-MS) in drug discovery and development. With chapters written by experts with a wide range of practical experience from the pharmaceutical industry, emphasis is placed on techniques and applications. The book also includes chapters on how to utilize LC-MS instrumentation for current drug metabolism problems. This book is intended for those beginning to use LC-MS for drug metabolism studies as well as for those considered advanced practitioners. * Introduces readers to the practical applications of modern liquid chromatography-mass spectrometry (LC-MS) in a wide range of drug metabolism studies?·* Provides a comprehensive description of different forms of metabolites, with detailed discussion on the wide range of methodologies used to identify them* Highlights problems associated with drug quantification and offers practical solutions Content: Preface Pages v-vi Swapan K. Chowdhury List of contributors Pages xv-xvi Chapter 1 LC-MS and drug metabolism: A journey back in time, present trends and future directions Original Research Article Pages 1-5 Kevin B. Alton Chapter 2 Strategies for increasing throughput for pk samples in a drug discovery environment Original Research Article Pages 7-34 Walter A. Korfmacher Chapter 3 Commonly encountered analytical problems and their solutions in liquid chromatography/ tandem mass spectrometry (LC/MS/MS) methods used in drug development Original Research Article Pages 35-64 Japan K. Majumdar Chapter 4 Pitfalls in quantitative LC-MS/MS: Metabolite contribution to measured drug concentration Original Research Article Pages 65-103 Mohammed Jemal Chapter 5 In vitro dmpk screening in drug discovery, role of lc-ms/ms Original Research Article Pages 105-122 Inhou Chu, Amin A. Nomeir Chapter 6 Metabolite identification by lc-ms: applications in drug discovery and development Original Research Article Pages 123-158 Cornells E.C.A. Hop, Chandra Prakash Chapter 7 The utility of in vitro screening for the assessment of electrophilic metabolite formation early in drug discovery using hplc-ms/ms Original Research Article Pages 159-182 Diane E. Grotz, Nigel A. Clarke, Kathleen A. Cox Chapter 8 Liquid chromatography tandem mass spectrometry-based metabolite identification strategies in pharmaceutical research Original Research Article Pages 183-194 M. Reza Anari, Philip R. Tiller, Thomas A. Baillie Chapter 9 Quantification and structural elucidation of low quantities of radiolabeled metabolites using microplate scintillation counting (msc) techniques in conjunction with lc-ms Original Research Article Pages 195-223 Mingshe Zhu, Donglu Zhang, Gary L. Skiles Chapter 10 Oxidative metabolites of drugs and xenobiotics: lc-ms methods to identify and characterize in biological matrices Original Research Article Pages 225-276 Ragulan Ramanathan, Swapan K. Chowdhury, Kevin B. Alton Chapter 11 Detection and characterization of highly polar metabolites by lc-ms: proper selection of lc column and use of stable isotope-labeled drug to study metabolism of ribavirin in rats Original Research Article Pages 277-293 S.K. Chowdhury, VS. Gopaul, N. Blumenkrantz, R. Zhong, K.M. Kulmatycki, K.B. Alton Chapter 12 Cytochrome p450 (cyp) and udp-glucuronosyltransferase (ugt) enzymes: role in drug metabolism, polymorphism, and identification of their involvement in drug metabolism Original Research Article Pages 295-336 Anima Ghosal, Ragulan Ramanathan, Narendra S. Kishnani, Swapan K. Chowdhury, Kevin B. Alton Subject index Pages 337-338