Hyper Bio Assembler for 3D Cellular Systems

Hyper Bio Assembler for Cellular Systems is the first book to present a new methodology for measuring and separating target cells at high speed and constructing 3D cellular systems in vitro. This book represents a valuable resource for biologists, biophysicists and robotic engineers, as well as researchers interested in this new frontier area, offering a better understanding of the measurement, separation, assembly, analysis and synthesis of complex biological tissue, and of the medical applications of these technologies. This book is the outcome of the new academic fields of the Ministry of Education, Culture, Sports, Science and Technology’s Grant-in-Aid for Scientific Research in Japan. Front Matter....Pages i-xi Front Matter....Pages 1-1 Cell Mechanical Characterization Based on On-Chip Robotics....Pages 3-22 Dimensionless Evaluation of Cell Deformability with High Resolution Positioning in a Microchannel....Pages 23-42 Real-time Capillary-level Microchannel Flow Analysis Using a Full-pixel Frame-straddling Micro-PIV System....Pages 43-55 High-throughput Measurements of Single Cell Rheology by Atomic Force Microscopy....Pages 57-67 Discrimination of Cells with Specific Antigens Expressed on a Membrane Based on the Dielectrophoresis....Pages 69-78 Analysis of Physical Characteristic of Hematopoietic Cells....Pages 79-90 Front Matter....Pages 91-91 Cell Manipulation and Cellular Parts Assembly for Constructing 3D Cellular Systems....Pages 93-128 High-Throughput Cell Assembly Featuring Heterogeneous Hydrogels Produced by Using Microfluidic Devices....Pages 129-150 On-Chip Fabrication, Manipulation and Self-Assembly for Three-Dimensional Cell Structures....Pages 151-176 Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies....Pages 177-202 Photofablication Techniques for 3D Tissue Construct....Pages 203-211 Cell Detachment for Engineering Three-Dimensional Tissues....Pages 213-222 Quantitative Evaluation of Cell-Hydrogel Adhesion by Advanced Optical Techniques....Pages 223-234 Cell Scooper: A Device for the Rapid Transfer of Living Cell Sheet....Pages 235-247 Front Matter....Pages 249-249 Hydrogel-Based Microenvironment for Modulating Gland Tissue Morphogenesis....Pages 251-259 Bone Related Cell-Stimulating Scaffold Materials and a 3D Cellular Construct for Hard Tissue Regeneration....Pages 261-273 The Visualization of Human Organogenesis from Stem Cells by Recapitulating Multicellular Interactions....Pages 275-283 Bionic Simulator Based on Organ-Explant-Chip....Pages 285-294 Tempo-Spatial Dynamics of Cellular Mechanics....Pages 295-304 Four-Dimensional Analysis for a Tumor Invasion....Pages 305-315 Front Matter....Pages 249-249 Three-Dimensional Mineralized Tissue Formation of Cultured Bone Marrow Stromal Cells....Pages 317-326 Sociocytology Illuminated by Reconstructing Functional Tissue with Cell Sheet Based Technology....Pages 327-345 Back Matter....Pages 347-349