Human Cell Culture: Volume IV: Primary Hematopoietic Cells (Human Cell Culture)

The daily production of hundreds of billions of blood cells through the process of hematopoiesis is a remarkable feat of human physiology. Transport of oxygen to tissues, blood clotting, antibody- and cellular-mediated immunity, bone remodeling, and a host of other functions in the body are dependent on a properly functioning hematopoietic system. As a consequence, many pathological conditions are attributable to blood cell abnormalities, and a fair number of these are now clinically treatable as a direct result of hematopoietic research. Proliferation of hematopoietic stem cells, and their differentiation into the many different lineages of functional mature cells, is highly regulated and responsive to many environmental and physiological challenges. Our relatively advanced understanding of this stem cell system provides potentially important insights into the regulation of development in other tissues, many of which are now being acknowledged as stem ce- based, perhaps even into adulthood. The recent public and scientific fanfare following announcement of human embryonic stem cell studies suggests that stem cell research will continue to be a relevant and exciting topic.

This unique comprehensive volume in the Human Cell Culture Series encompasses research methodology for the growth and differentiation of all types of primary hematopoietic cells. Each of the twelve hematopoietic cell types is covered by a leading expert in the field, providing insightful background information along with detailed current culture and assay techniques. In addition to uses for research applications, current and future clinical applications of large-scale culture methods are also discussed. Further, the procurement and processing of primary human tissues is covered in detail. Because of the regulatory and ethical implications associated with use of human and fetal tissues, a separate chapter is included to guide scientists through these issues as well. The generous inclusion of figures and tables, along with a consistent format, makes critical information highly accessible to the reader. Additionally, numerous literature citations provide a valuable reference for students and professionals in the hematology, immunology, oncology and bioengineering fields.

Booknews

Describes research technology for the growth and differentiation of all 12 types of the primary hematopoietic cells that develop into the various types of blood cells. Also provides background information, discusses current and future clinical applications of large-scale culture methods, and considers regulatory and ethical implications associated with using human and fetal tissues. The 13 studies include treatments of hematopoietic stem and progenitor cells, monocytes and macrophages, isolating and culturing human dendritic cells, purifying and culturing erythroid progenitor cells, the development of megakaryocytes and platelets, the mature polyclonal and antigen- specific cell expansion of T-lymphocytes, and T- lymphopoiesis. They are not indexed. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Hematopoietic Stem and Progenitor Cells....Pages 1-30 In Vitro T-Lymphopoiesis....Pages 31-44 T-Lymphocytes: Mature Polyclonal and Antigen-Specific Cell Culture....Pages 45-100 The Culture, Characterization and Triggering of B-Lymphocytes....Pages 101-124 Monocytes and Macrophages....Pages 125-145 Isolation and Cultivation of Osteoclasts and Osteoclast-Like Cells....Pages 147-169 Isolation and Culture of Dendritic Cells....Pages 171-191 In Vitro Proliferation and Differentiation of CD34+ Cells to Neutrophils....Pages 193-217 Isolation and Culture of Eosinophils....Pages 219-239 Isolation and Culture of Mast Cells and Basophils....Pages 241-257 Purification and Culture of Erythroid Progenitor Cells....Pages 259-286 In Vitro Development of Megakaryocytes and Platelets....Pages 287-315 Perspectives, Ethics and Clinical Issues in the Use of Primary Human Cells....Pages 317-342 "This unique comprehensive volume encompasses research methodology for the growth and differentiation of all types of primary hematopoietic cells. Each of the 12 hematopoietic cell types is covered by a leading expert in the field, providing insightful background information along with detailed current culture and assay techniques. In addition to uses for research applications, current and future clinical applications of large-scale culture methods are also discussed." The human body consumes a staggering 400 billion mature blood cells every day, and this number increases dramatically under conditions of stress such as infection or bleeding.