Hormone Action, Part J: Neuroendocrine Peptides, Volume 124: Volume 124: Hormone Action Part J (Methods in Enzymology)

The critically acclaimed laboratory standard, **Methods in Enzymology**, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences Content: Contributors to volume 124 Pages ix-xiii Preface Pages xv-xvi P. Michael Conn Volumes in series Pages xvii,xix-xxviii [1] Computationally directed biorational drug design of peptides Original Research Article Pages 3-18 Frank A. Momany, Hiroshi Chuman [2] Comparative methods for the radiolabeling of peptides Original Research Article Pages 18-29 A.E. Bolton [3] Luciferin derivitization of ligands for development of nonisotopic binding assays Original Research Article Pages 29-35 James M. Schaeffer [4] Synthesis and use of colloidal gold-coupled receptor ligands Original Research Article Pages 36-47 Lothar Jennes, P. Michael Conn, Walter E. Stumpf [5] Preparation and use of biotinylated neuroendocrine peptides Original Research Article Pages 47-57 Eli Hazum [6] Synthesis and use of diacylglycerols as activators of protein kinase C in neuroendocrine tissue Original Research Article Pages 57-63 P. Michael Conn, Barry R. Ganong, James Ebeling, Daphne Staley, James E. Niedel, Robert M. Bell [7] Computer-controlled perifusion system for neuroendocrine tissues: Development and applications Original Research Article Pages 67-79 Andres Negro-Vilar, Michael D. Culler [8] Perifusion of anterior pituitary cells: Release of conadotropins and somatotropins Original Research Article Pages 79-90 Thomas M. Badger [9] Measurement of intracellular ionized calcium with aequorin Original Research Article Pages 90-116 AndrГ©B. Borle, Kenneth W. Snowdowne [10] Use of intracellular calcium and membrane potential fluorescent indicators in neuroendocrine cells Original Research Article Pages 116-135 Alessandro M. Capponi, P. Daniel Lew, Werner Schlegel, Tullio Pozzan [11] The design and synthesis of detergents for membrane biochemistry Original Research Article Pages 135-164 Leonard M. Hjelmeland [12] Solubilization of neuropeptide receptors Original Research Article Pages 164-171 Marilyn H. Perrin [13] Solubilization and characterization of opiate receptors Original Research Article Pages 172-190 R. Suzanne Zukin, Rhoda Maneckjee [14] Temperature measurement and control of small volumes: Applications for single channel recording Original Research Article Pages 190-206 Anthony Auerbach, Frederick Sachs, James Neil, Richard McGarrigle [15] Techniques for studying the role of electrical activity in control of secretion by normal anterior pituitary cells Original Research Article Pages 207-242 W.T. Mason, C.D. Ingram [16] Culture and characterization of follicular cells of the bovine anterior pituitary and pars tuberalis Original Research Article Pages 245-253 Napoleone Ferrara, Paul Goldsmith, Dennis Fujii, Richard Weiner [17] Peptidergic secretory granules isolated from the brain: A model system for study of intracellular aspects of the process of peptide secretion Original Research Article Pages 254-265 Ayalla Barnea [18] RNA dot and blot hybridization: Selected procedures for endocrine and neuroendocrine studies Original Research Article Pages 269-278 Bruce A. White, Thomas Lufkin, Gregory M. Preston, Carter Bancroft [19] Gene transfer methods for studying the regulation and expression of neuropeptide genes Original Research Article Pages 278-294 Audrey Seasholtz, Michael Comb, Michel Martin, Edward Herbert [20] Use of vaccinia virus as a neuropeptide expression vector Original Research Article Pages 295-309 Dennis E. Hruby, Gary Thomas, Edward Herbert, Christine A. Franke [21] Detection of neuroendocrine peptide precursor cDNA clones using synthetic oligonucleotides Original Research Article Pages 309-318 James Douglas, Edward Herbert [22] Detection of a high-molecular-weight LHRH precursor by cell-free translation of mRNA from human, rat, and mouse hypothalamus Original Research Article Pages 318-335 Ann Curtis, Michael Szelke, George Fink [23] Methods for the identification of neuropeptide processing products: Somatostatin and the tachykinins Original Research Article Pages 335-348 Anthony J. Harmar, Peter M. Keen [24] Assay and purification of protein kinase C Original Research Article Pages 349-352 Tatsuro Kitano, Masayoshi Go, Ushio Kikkawa, Yasutomi Nishizuka [25] Mixed micelle assay of protein kinase C Original Research Article Pages 353-359 Robert M. Bell, Yusuf Hannun, Carson Loomis [26] A tissue culture model for the study of peptide synthesis and secretion from microdissected hypothalamic explants Original Research Article Pages 359-371 Mariana Morris, Robert L. Eskay, David K. Sundberg [27] Measurement of growth hormone-releasing factor Original Research Article Pages 371-389 Lawrence A. Frohman, Thomas R. Downs [28] Assay of growth hormone-releasing factor Original Research Article Pages 389-402 Wylie Vale, Joan Vaughan, Diane Jolley, Gayle Yamamoto, Thomas Bruhn, Hans Seifert, Marilyn Perrin, Michael Thorner, Jean Rivier [29] Microbore HPLC of biogenic amines in small biological samples Original Research Article Pages 402-412 Ivan N. Mefford, Edward J. Caliguri, Richard K. Grady Jr., Peter Capella, Tracy A. Durkin, Pamela Chevalier [30] Somatostatin release from dissociated cerebral cortical cell cultures Original Research Article Pages 412-424 Roxanne M. Landon, Richard J. Robbins [31] Measurement of phospholipid turnover in cultured hormone responsive pituitary cells Original Research Article Pages 424-442 Thomas F.J. Martin [32] Detection and measurement of secretion from individual neuroendocrine cells using a reverse hemolytic plaque assay Original Research Article Pages 443-465 Philip F. Smith, Enrique H. Luque, Jimmy D. Neill [33] Measurement of neuropeptide release: In vitro and in vivo procedures Original Research Article Pages 466-494 J.E. Levine, V.D. Ramirez [34] In Situ hybridization for the study of gene expression in the brain Original Research Article Pages 497-510 Brenda D. Shivers, Beth S. Schachter, Donald W. Pfaff [35] In Situ cDNA;mRNA hybridization: Development of technique to measure mRNA levels in individual cells Original Research Article Pages 510-533 Josiah N. Wilcox, Connie E. Gee, James L. Roberts [36] Location of gene expression in CNS using hybridization histochemistry Original Research Article Pages 534-548 J.D. Penschow, J. Haralambidis, P. Aldred, G.W. Tregear, J.P. Coghlan [37] Measurement of neuroendocrine peptide mRNA in discrete brain regions Original Research Article Pages 548-560 Jeffrey D. White, Kim D. Stewart, Jeffrey F. McKelvy [38] Corticotropin-releasing factor receptors in the pituitary gland and central nervous system: Methods and overview Original Research Article Pages 560-590 Errol B. De Souza, Michael J. Kuhar [39] Autoradiographic localization of brain receptors for peptide hormones: Angiotensn II, corticotropin-releasing factor, and gonadotropin-releasing hormone Original Research Article Pages 590-606 Monica Millan, Greii Aguilera, Peter C. Wynn, Frederick A.O. Mendelsohn, Kevin J. Catt [40] Localization of somatostatin receptors Original Research Article Pages 607-617 G. Pelletier, P. Leroux, G. Morel [41] Methods for estimating the contribution of proenkephalin A and proenkephalin B input to neuronal areas Original Research Article Pages 617-626 David K. Sundberg, Claud E. Dunlap III [42] Previously published articles from methods in enzymology related to neuroendocrine peptides Page 629 Author index Pages 631-656 Subject index Pages 657-679