Hormone Action, Part I: Peptide Hormones, Volume 109: Volume 109: Hormone Action Part I (Methods in Enzymology)

The critically acclaimed laboratory standard, **Methods in Enzymology**, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences Content: Contributors to volume 102 Pages ix-xi Preface Page xiii Anthony R. Means, Bert W. O'Malley Volumes in series Pages xv,xvii-xxiv [1] Calmodulin purification and fluorescent labeling Original Research Article Pages 1-8 John R. Dedman, Marcia A. Kaetzel [2] Purification of plant calmodulin Original Research Article Pages 9-17 James Michael Anderson [3] Purification of calmodulin by Ca 2+ -dependent affinity chromatography Original Research Article Pages 17-39 Harry Charbonneau, Rita Hice, Russell C. Hart, Milton J. Cormier [4] Assay of calmodulin by Ca 2+ -dependent phosphodiesterase Original Research Article Pages 39-47 Robert W. Wallace, E. Ann Tallant, Wai Yiu Cheung [5] The red blood cell as a model for calmodulin-dependent Ca 2+ transport Original Research Article Pages 47-62 Thomas R. Hinds, Frank F. Vincenzi [6] Myosin light chain phosphorylation in smooth muscle and nonmuscle cells as a probe of calmodulin function Original Research Article Pages 62-74 Paul J. Silver, James T. Stull [7] Assessment of Ca 2+ -calmodulin formation in intact vertebrate skeletal muscle Original Research Article Pages 74-82 David R. Manning, James T. Stull [8] Spectroscopic analyses of calmodulin and its interactions Original Research Article Pages 82-104 Rachel E. Klevit [9] Production of polyclonal and monoclonal antibodies to calmodulin and utilization of these immunological probes Original Research Article Pages 104-110 James G. Chafouleas, Mary E. Riser, Lisette LagacГ©, Anthony R. Means [10] Localization of calmodulin and calmodulin acceptor sites by fluorescence methods Original Research Article Pages 110-121 Michael J. Welsh [11] Immunofluorescence localization of calmodulin in unfixed frozen tissue sections Original Research Article Pages 122-135 Jeffrey F. Harper, Alton L. Steiner [12] Ca 2+ binding to calmodulin Original Research Article Pages 135-143 James D. Potter, Priscilla Strang-Brown, Patricia L. Walker, Shozo Iida [13] Preparation of calmodulin crystals Original Research Article Pages 143-147 William J. Cook, John S. Sack [14] Preparation of fluorescent labeled calmodulins Original Research Article Pages 148-157 Bradley B. Olwin, Daniel R. Storm [15] Posttranslational modification of calmodulin Original Research Article Pages 158-170 Timothy J. Murtaugh, Paul M. Rowe, Pamela L. Vincent, Lynda S. Wright, Frank L. Siegel [16] Techniques for measuring the interaction of drugs with calmodulin Original Research Article Pages 171-184 Benjamin Weiss [17] Naphthalenesulfonamides as calmodulin antagonists Original Research Article Pages 185-194 Hiroyoshi Hidaka, Toshio Tanaka [18] Synthesis and characterization of calmodulin antagonistic drugs Original Research Article Pages 195-204 Russell C. Hart, Michael D. Bates, Milton J. Cormier, Gerald M. Rosen, P. Michael Conn [19] Detection of calmodulin-binding polypeptides separated in SDS-polyacrylamide gels by a sensitive [ 125 I]calmodulin gel overlay assay Original Research Article Pages 204-210 John R. Glenney Jr., Klaus Weber [20] Use of calmodulin affinity chromatography for purification of specific calmodulin-dependent enzymes Original Research Article Pages 210-219 Rajendra K. Sharma, William A. Taylor, Jerry H. Wang [21] Calmodulin as an integral subunit of phosphorylase kinase from rabbit skeletal muscle Original Research Article Pages 219-227 Colin Picton, Shirish Shenolikar, Roger Grand, Philip Cohen [22] Isolation and characterization of bovine brain calcineurin: A calmodulin-stimulated protein phosphatase Original Research Article Pages 227-244 C.B. Klee, M.H. Krinks, A.S. Manalan, P. Cohen, A.A. Stewart [23] Purification and radioimmunoassay of calmodulin-dependent protein phosphatase from bovine brain Original Research Article Pages 244-256 E. Ann Tallant, Robert W. Wallace, Wai Yiu Cheung [24] Assay of S-100 protein by an enzyme immunoassay method Original Research Article Pages 256-261 Hiroyoshi Hidaka, Toyoshi Endo, Kanefusa Kato [25] Covalent regulation of the cardiac sarcoplasmic reticulum calcium pump: Purification and properties of phospholamban, a substrate of cAMP-dependent protein kinase and Ca 2+ -calmodulin-dependent phospholamban kinase Original Research Article Pages 261-278 Christian J. Le Peuch, Danielle A.-M. Le Peuch, Jacques G. Demaille [26] Ca 2+ -dependent neutral protease and proteolytic activation of Ca 2+ -activated, phospholipid-dependent protein kinase Original Research Article Pages 279-290 Norio Kajikawa, Akira Kishimoto, Makoto Shiota, Yasutomi Nishizuka [27] Enzyme-linked immunoabsorbent assay (ELISA) and radioimmunoassay (RIA) for the vitamin D-dependent 28,000 dalton calcium-binding protein Original Research Article Pages 291-296 Barbara E. Miller, Anthony W. Norman [28] Chemical approaches to the calmodulin system Original Research Article Pages 296-310 Thomas C. Vanaman Author index Pages 311-320 Subject index Pages 321-336