HLA from Benchtop to Bedside

__HLA from Benchtop to Bedside provides__ the reader with a comprehensive, concise and thoroughly up-to-date book on all aspects of the HLA system, including new techniques and methodologies. Each chapter begins with bullet point lists of principle learning points, including comprehensive references and validated links to international resources. Written by a diverse range of international academics for professionals, researchers, undergraduate and graduate students, this book is ideal for organ and stem cell transplant professionals, histocompatibility laboratory professionals and staff, medical residents and fellows on transplant services, medical students, and students in clinical laboratory science. The book's author, Dr. Arthur Bradley Eisenbrey, is an experienced transplant pathologist who has held significant academic and leadership positions in the field. Front-Matter_2021_HLA-from-Benchtop-to-Bedside HLA from Benchtop to Bedside Copyright_2021_HLA-from-Benchtop-to-Bedside Copyright Preface_2021_HLA-from-Benchtop-to-Bedside Preface Acknowledgments_2021_HLA-from-Benchtop-to-Bedside Acknowledgments Chapter-1---Introduction-and-some-history_2021_HLA-from-Benchtop-to-Bedside 1 . Introduction and some history Fundamentals: innate and adaptive immunity References Chapter-2---The-structure-of-the-major-histocompatibil_2021_HLA-from-Benchto 2 . The structure of the major histocompatibility complex and major HLA components HLA class I Function of HLA Class Ia molecules HLA Ib molecules Other HLA class I histocompatibility genes: MICA and MICB HLA class II References Chapter-3---Nomenclature-and-naming-conventions_2021_HLA-from-Benchtop-to-Be 3 . Nomenclature and naming conventions for HLA HLA allele naming References Chapter-4---The-crossmatch_2021_HLA-from-Benchtop-to-Bedside 4 . The crossmatch Microcytotoxicity and the ``NIH standard'' technique Antihuman globulin Crossmatching by microcytotoxicity ABO and solid organ transplantation T and B cell isolation Immunofluorescence Flow cytometry HLA antibody detection in tissues References Further reading Chapter-5---HLA-antibody-detection--screening--an_2021_HLA-from-Benchtop-to- 5 . HLA antibody detection (screening) and identification Antibody identification by microlymphocytotoxicity Interpretation of antibody specificity in cell-based assays Panel reactive antibody Solid-phase antibody screening and identification Commercial solid-phase HLA antibody assays are qualitative, not quantitative Clinical indications for HLA antibody testing Single antigen bead result interpretation in clinical practice MFI as a quantitative result Virtual crossmatch Management of antibody-mediated transplant rejection HLA in Transfusion Medicine and Blood Banking TRALI Platelet transfusion support References Chapter-6---HLA-typing-by-serologic-methods_2021_HLA-from-Benchtop-to-Bedsid 6 . HLA typing by serologic methods References Chapter-7---HLA-typing-by-molecular-methods_2021_HLA-from-Benchtop-to-Bedsid 7 . HLA typing by molecular methods Molecular HLA typing ``before PCR'' Sequencing Oligonucleotide probing and endonuclease cutting Restriction fragment length polymorphism Polymerase chain reaction and the molecular revolution in clinical medicine Polymerase chain reaction sequence specific primers PCR-RFLP PCR-rSSO High resolution typing ambiguities in molecular HLA typing The challenge of null alleles rtPCR (aka qPCR, real-time PCR, and quantitative PCR) References Chapter-8---Massively-parallel--next-generation-_2021_HLA-from-Benchtop-to-B 8 . Massively parallel (next generation) sequencing NGS sequencing by Hybridization and Ligation Selection of molecular HLA typing methods References Chapter-9---HLA-alleles--disease-associations--and-_2021_HLA-from-Benchtop-t 9 . HLA alleles, disease associations, and HLA pharmacogenetics The mathematics of disease associations How do HLA alleles cause disease? References Chapter-10---Engraftment-monitoring_2021_HLA-from-Benchtop-to-Bedside 10 . Engraftment monitoring A little history Microsatellite DNA, VNTRs, STRs Engraftment monitoring Engraftment monitoring by capillary electrophoresis Alternative platforms for engraftment monitoring References Further reading Index_2021_HLA-from-Benchtop-to-Bedside Index A B C D E F G H I K L M N O P R S T U V HLA from Benchtop to Bedside provides the reader with a comprehensive, concise and thoroughly up-to-date book on all aspects of the HLA system, including new techniques and methodologies. Each chapter begins with bullet point lists of principle learning points, including comprehensive references and validated links to international resources. Written by a diverse range of international academics for professionals, researchers, undergraduate and graduate students, this book is ideal for organ and stem cell transplant professionals, histocompatibility laboratory professionals and staff, medical residents and fellows on transplant services, medical students, and students in clinical laboratory science. The book's author, Dr. Arthur Bradley Eisenbrey, is an experienced transplant pathologist who has held significant academic and leadership positions in the field. Reviews current knowledge surrounding the HLA system Covers current methodologies and utilization of histocompatibility testing Authored by a leader in the field of histocompatibility and transfusion medicine