HIV Interactions with Host Cell Proteins (Current Topics in Microbiology and Immunology, 339)

The Study Of Viruses Necessarily Involves Dissecting The Intimate Details Of Cellular Pathways. Viruses Have Often Been Employed As Tools In Studying Cellular Pathways, As Was Done By Early Retrovirologists Such As Peyton Rous In Attempting To Understand The Mechanism Of Cellular Transformation And Oncogenesis. On The Other Side Of The Coin, Virologists Seek To De?ne Those Cellular Elements Interacting Intimatelywiththeir Virus Ofinterestinorder To Better Understand Viral Replication Itself, And In Some Cases To Develop Antiviral Strategies. It Is In The Intersection Of Virology And Cell Biology That Many Of Us ?nd The Most Rewarding Aspects Of Our Research. When A New Discovery Yields Insights Into Basic Cellular Mechanisms And Presents New Targets For Int- Vention To ?ght A Serious Pathogen, The Impact Can Be High And The Excitement Intense. Hiv Has Been No Exception To The Rule That Viruses Reveal Many Basic Aspects Of Cellular Biology. In Recent Years, In Part Because Of The Importance Of Hiv As A Major Cause Of Human Suffering, Numerous Cellular Processes Have Been Elucidated Through Work On Processes Or Proteins Of This Human Retrovirus. The Excitement In This ?eld Is Especially Well Illustrated By The Discovery Of New Innate Means Of Resisting Viral Replication, Such As The Work On Apobec3g, Trim5a, And Bst-2/ Tetherin Presented In This Volume. Front Matter....Pages 1-9 Host Restriction of HIV-1 by APOBEC3 and Viral Evasion Through Vif....Pages 1-25 Interactions of Viral protein U (Vpu) with Cellular Factors....Pages 27-45 TRIM5alpha....Pages 47-66 Human Immunodeficiency Virus Type-1 Gag and Host Vesicular Trafficking Pathways....Pages 67-84 The Roles of Tetraspanins in HIV-1 Replication....Pages 85-102 Imaging of HIV/Host Protein Interactions....Pages 103-123 Virological and Cellular Roles of the Transcriptional Coactivator LEDGF/p75....Pages 125-146 Implications of Nef: Host Cell Interactions in Viral Persistence and Progression to AIDS....Pages 147-175 Vpr and Its Interactions with Cellular Proteins....Pages 177-200 Back Matter....Pages 1-4 Preface -- Host Restriction Of Hiv-1 By Apobec3 And Viral Evasion Through Vif -- Interactions Of Viral Protein U (vpu) With Cellular Factors -- Trim5alpha -- Human Immunodeficiency Virus Type-1 Gag And Host Vesicular Trafficking Pathways -- The Roles Of Tetraspanins In Hiv-1 Replication -- Imaging Of Hiv/host Protein Interactions -- Virological And Cellular Roles Of The Transcriptional Coactivator Ledgf/p75 -- Implications Of Nef: Host Cell Interactions In Viral Persistence And Progression To Aids -- Vpr And Its Interactions With Cellular Proteins Paul Spearman, Eric O. Freed, Editors. Includes Bibliographical References And Index. The authors seek to understand the assembly process of human immunodeficiency virus (HIV) and to develop a vaccine that can effectively neutralize HIV. HIV assembly is a process directed by the viral Gag polyprotein. Gag is a myristoylated precursor protein that is translated in the cytoplasm and then traffics to the plasma membrane or to endosomal vesicles for assembly. The authors have recently described an interaction between Gag and the delta subunit of the AP-3 adaptor protein complex (1). The mechanism and structural basis for this interaction is now under intense study. Our hypothesis i