Handbook of Single-Molecule Biophysics[ HANDBOOK OF SINGLE-MOLECULE BIOPHYSICS ] by Hinterdorfer, Pe

The last decade has seen the development of a number of novel biophysical methods that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at this fundamental level of sensitivity has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The __Handbook of Single-Molecule Biophysics__ provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them. Coverage includes: * Experimental techniques to monitor and manipulate individual biomolecules * The use of single-molecule techniques in super-resolution and functional imaging * Single-molecule studies of physical properties of biomolecules * Single-molecule enzymology and biochemistry * Single molecules in the membrane * Single-molecule techniques in living cells * Integration of single-molecule biophysics and nanoscience Single-molecule Fluorescent Particle Tracking / Ahmet Yidiz -- Single-molecule Analysis Of Biomembranes / Thomas Schmidt And Gerhard J. Schutz -- Single-molecule Imaging In Live Cells / Jie Xiao -- Fluorescence Imaging At Sub-diffraction-limit Resolution With Stochastic Optical Reconstruction Microscopy / Graham T. Dempsey, Wenqin Wang, And Xiaowei Zhuang -- Single-molecule Fret: Methods And Biological Applications / Ling Chin Whang [and Others] -- Single-molecule Enzymology / Joseph J. Loparo And Antoine Van Oijen -- Single-molecule Studies Of Rotary Molecular Motors / Teuta Pilizota, Yoshiyuki Sowa, And Richard M. Berry -- Fluorescence Correlation Spectroscopy In Living Cells / Thomas Weidemann And Petra Schwille -- Precise Measurements Of Diffusion In Solution By Fluorescence Correlations Spectroscopy / Jorg Enderlein -- Single-molecule Studies Of Nucleic Acid Interactions Using Nanopores / Meni Wanunu, Gautam V. Soni, And Amit Meller --^ Nanopores: Generation, Engineering, And Single-molecule Applications / Stefan Howorka And Zuzanna Siwy -- Single-molecule Manipulation Using Optical Traps / Michael T. Woodside And Megan T. Valentine -- Magnetic Tweezers For Single-molecule Experiments / I.d. Vilfan [and Others] -- Folding Of Proteins Under Mechanical Force / Michael Schilerf And Matthias Rief -- Probing The Energy Landscape Of Protein-binding Reactions By Dynamic Force Spectroscopy / Andreas Ebner [and Others] -- Probing Single Membrane Proteins By Atomic Force Microscopy / S. Scheuring, K. Tanuj Sapra, And Daniel J. Muller -- High-speed Atomic Force Microscopy / Toshio Ando And Takayuki Uchihashi -- Recognition Imaging Using Atomic Force Microscopy / Andreas Ebner [and Others] -- Atomic Force Microscopy Of Protein -- Protein Interactions / Xiaohui Zhang [and Others] -- A New Approach To Analysis Of Single-molecule Force Measurements / Evan Evans [and Others] --^ Single-molecule Recognition: Extracting Information From Individual Binding Events And Their Correlation / Cheng Zhu [and Others]. Peter Hinterdorfer, Antoine Van Oijen, Editors. Includes Bibliographical References And Index. Single-molecule fluorescent particle tracking / Ahmet Yidiz Single-molecule analysis of biomembranes / Thomas Schmidt and Gerhard J. Schutz Single-molecule imaging in live cells / Jie Xiao Fluorescence imaging at sub-diffraction-limit resolution with stochastic optical reconstruction microscopy / Graham T. Dempsey, Wenqin Wang, and Xiaowei Zhuang Single-molecule FRET: methods and biological applications / Ling Chin Whang ... [et al.] Single-molecule enzymology / Joseph J. Loparo and Antoine van Oijen Single-molecule studies of rotary molecular motors / Teuta Pilizota, Yoshiyuki Sowa, and Richard M. Berry Fluorescence correlation spectroscopy in living cells / Thomas Weidemann and Petra Schwille Precise measurements of diffusion in solution by fluorescence correlations spectroscopy / Jorg Enderlein Single-molecule studies of nucleic acid interactions using nanopores / Meni Wanunu, Gautam V. Soni, and Amit Meller Nanopores: generation, engineering, and single-molecule applications / Stefan Howorka and Zuzanna Siwy Single-molecule manipulation using optical traps / Michael T. Woodside and Megan T. Valentine Magnetic tweezers for single-molecule experiments / I.D. Vilfan ... [et al.] Folding of proteins under mechanical force / Michael Schilerf and Matthias Rief Probing the energy landscape of protein-binding reactions by dynamic force spectroscopy / Andreas Ebner ... [et al.] Probing single membrane proteins by atomic force microscopy / S. Scheuring, K. Tanuj Sapra, and Daniel J. Muller High-speed atomic force microscopy / Toshio Ando and Takayuki Uchihashi Recognition imaging using atomic force microscopy / Andreas Ebner ... [et al.] Atomic force microscopy of protein - protein interactions / Xiaohui Zhang ... [et al.] A new approach to analysis of single-molecule force measurements / Evan Evans ... [et al.] Single-molecule recognition: extracting information from individual binding events and their correlation / Cheng Zhu ... [et al.]. Front Matter....Pages i-xxi Single-Molecule Fluorescent Particle Tracking....Pages 1-18 Single-Molecule Analysis of Biomembranes....Pages 19-42 Single-Molecule Imaging in Live Cells....Pages 43-93 Fluorescence Imaging at Sub-Diffraction-Limit Resolution with Stochastic Optical Reconstruction Microscopy....Pages 95-127 Single-Molecule FRET: Methods and Biological Applications....Pages 129-163 Single-Molecule Enzymology....Pages 165-182 Single-Molecule Studies of Rotary Molecular Motors....Pages 183-216 Fluorescence Correlation Spectroscopy in Living Cells....Pages 217-241 Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy....Pages 243-263 Single-Molecule Studies of Nucleic Acid Interactions Using Nanopores....Pages 265-291 Nanopores: Generation, Engineering, and Single-Molecule Applications....Pages 293-339 Single-Molecule Manipulation Using Optical Traps....Pages 341-370 Magnetic Tweezers for Single-Molecule Experiments....Pages 371-395 Folding of Proteins under Mechanical Force....Pages 397-406 Probing the Energy Landscape of Protein-Binding Reactions by Dynamic Force Spectroscopy....Pages 407-447 Probing Single Membrane Proteins by Atomic Force Microscopy....Pages 449-485 High-Speed Atomic Force Microscopy....Pages 487-523 Recognition Imaging Using Atomic Force Microscopy....Pages 525-554 Atomic Force Microscopy of Protein–Protein Interactions....Pages 555-570 A New Approach to Analysis of Single-Molecule Force Measurements....Pages 571-589 Single-Molecule Recognition: Extracting Information from Individual Binding Events and Their Correlation....Pages 591-610 Back Matter....Pages 611-626 This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.