Handbook of Isoelectric Focusing and Proteomics (Volume 7) (Separation Science and Technology, Volume 7)

Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics Content: Preface Pages xi-xiii David Garfin, Satinder Ahuja Contributors Pages xv-xvi Incidents of travel in IEF and IPGS Pages xvii-xxii Pier Giorgio Righetti I Overview Original Research Article Pages 1-12 David E. Garfin, Satinder Ahuja 2 Evolution and development of isoelectric focusing Original Research Article Pages 13-39 Ákos Végvári, Ferenc Kilár 3 Theory and simulation of isoelectric focusing Original Research Article Pages 41-68 T.L. Sounart, P.A. Safier, J.C. Baygents 4 Generation of pH gradients Original Research Article Pages 69-92 Tom Berkelman 5 Slab gel IEF Original Research Article Pages 93-122 Reiner Westermeier 6 Two-dimensional gel electrophoresis Original Research Article Pages 123-145 Mark P. Molloy, Michael T. McDowell 7 Some practices and pitfalls of sample preparation for isoelectric focusing in proteomics Original Research Article Pages 147-164 Ben Herbert 8 Protein detection and imaging in IEF gels Original Research Article Pages 165-179 Wayne F. Patton 9 Capillary isoelectric focusing Original Research Article Pages 181-210 Tim Wehr 10 Free-flow isoelectric focusing Original Research Article Pages 211-245 Peter J.A. Weber, Gerhard Weber, Christoph Eckerskorn, Ulrich Schneider, Anton Posch 11 Isoelectric focusing and proteomics Original Research Article Pages 247-264 Melanie Y. White, Stuart J. Cordwell 12 Chromatofocusing Original Research Article Pages 265-296 David Anderson 13 Alternative electrofocusing methods Original Research Article Pages 297-319 Cornelius F. Ivory Index Pages 321-334 The emergence of proteomics as a mature discipline has brought renewed interest in isoelectric focusing (IEF), as it is a rapid and reliable technique that provides high resolution for protein fractionation. The Handbook of Isoelectric Focusing and Proteomics is intended to serve as a single source for all relevant information on IEF.Each chapter includes special insights and practical tips from the experts. The subject matter is arranged in a logical order, from the history of IEF through alternative forms of focusing: evolution of the technique; theoretical considerations of IEF; generation of pH gradients; practical aspects of slab gel IEF; 2-DE methodology; important considerations in sample preparation; detection methods for visualizing proteins in IEF gels, including image acquisition and analysis; various techniques of capillary IEF; free-flow electrophoresis and its use as a preparative technique; role of IEF in 2-D gel-based proteomics; chromatofocusing; and alternate electrofocusing methods that can point to possible routes to more efficient techniques for fractionating and purifying proteins.Anyone working with proteins, from the level of graduate student to senior researcher, will be able to find something useful in this book. The text emphasizes a hands-on approach to IEF and is slanted toward proteomics, since that is currently considered the most useful approach to protein biochemistry. From the viewpoint of separation technology, IEF is very impressive in resolving capabilities, yet elegant in its operational simplicity.