Handbook of Industrial Cell Culture : Mammalian, Microbial, and Plant Cells

The advent of molecular genetics, the progression of transgenic animal and plant methodologies from the laboratory to industrial-scale production, and the development of powerful analytical techniques have each helped standardize the culturing of cells to produce desirable commercial products. In Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells, a diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotechnological and industrial uses. Special emphasis is given to solving the critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery. The authors all have proven track records in the successful implementation of commercial-scale processes. Cutting-edge and highly practical, the Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells not only surveys the best current practices in the art of industrial cell culture across cell types, but also delineates today's evolving technologies with examples of proven practical applications, as well as projections of their future potential. Front Matter....Pages i-x Front Matter....Pages 1-1 Analysis and Manipulation of Recombinant Glycoproteins Manufactured in Mammalian Cell Culture....Pages 3-20 Genetic Approaches to Recombinant Protein Production in Mammalian Cells....Pages 21-49 Protein Expression Using Transgenic Animals....Pages 51-67 Mammalian Cell Culture....Pages 69-103 Front Matter....Pages 105-105 Natural Products....Pages 107-136 Genetic Engineering Solutions for Natural Products in Actinomycetes....Pages 137-170 Culture Medium Optimization and Scale-Up for Microbial Fermentations....Pages 171-193 Front Matter....Pages 195-195 Functional Genomics for Plant Trait Discovery....Pages 197-216 Molecular Tools for Engineering Plant Cells....Pages 217-241 Plant Cell Culture....Pages 243-258 Expression of Recombinant Proteins via the Plastid Genome....Pages 259-278 Oleosin Partitioning Technology for Production of Recombinant Proteins in Oil Seeds....Pages 279-295 Front Matter....Pages 297-297 Genome Sequencing and Genomic Technologies in Drug Discovery and Development....Pages 299-319 Proteomics....Pages 321-348 Metabolic Flux Analysis, Modeling, and Engineering Solutions....Pages 349-392 Advances in Analytical Chemistry for Biotechnology....Pages 393-464 DNA Shuffling for Whole Cell Engineering....Pages 465-481 Cell Culture Preservation and Storage for Industrial Bioprocesses....Pages 483-514 Back Matter....Pages 525-536

A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.

Mammalian cell culture has progressed in recent years from a lab-scale enterprise, mainly used for in vitro testing of chemical agents, to a major source of therapeutic and prophylactic agents.