Handbook of Drug Screening (Drugs and the Pharmaceutical Sciences)

This reference includes discussions of current screening techniques, the technologies involved, and high capacity instrumentation for the development of new drugs, chemical compounds, and the targeted delivery of pharmaceuticals. Twenty-one papers address topics like high throughput screening assays, screening platforms, homogenous assays, microbe-based screening systems, receptor screens, functional assay screens, enzyme screens, ion channel targets, drug discovery, bioinformatics, laboratory automation, and miniaturization. Contributors include researchers for companies in the United States, Canada, Germany, and France. Machine generated contents note: 1. Moving Into the Third Millennium After a Century of Screening Prabhavathi B. Ferandes 2. Basic Considerations in Designing High-Throughput Screening Assays Duane Bronson, Nathaniel Hentz, William P. Janzen, Mark D. Lister, Karl Menke, Jeffrey Wegrzyn, and G. Sitta Sittampalam 3 Screening Platforms Ramakrishna Seethala Homogeneous Assays for High-Throughput and Ultrahigh- Throughput Screening Ramakrishna Seethala Microbe-Based Screening Systems 'rabhavathi B. Ferandes 6. Molecular Genetic Screen Design for Agricultural and Pharmaceutical Product Discovery 152 Donald R. Kirsch, William Baumbach, Julia N. Heinrich, Margaret Lai, Mark H. Pausch, Laura Sarokin, Sanford Silverman, and James C. Walsh 7. Receptor Screens for Small Molecule Agonist and Antagonist Discovery 1I Ramakrishna Seethala 8. Functional Assay Screens 2 Maria L. Webb, Robert A. Horlick, Bassam Damaj, and Kirk McMillan 9. Enzyme Screens Thomas D. Y. Chung and Dennis J. Murphy 10. Screening Strategies for Ion Channel Targets Thomas H. Large and Martin W. Smith 11. High-Throughput Screening Assays for Detection of Transcription Mohanram Sivaraja 12. Screening of Combinatorial Biology Libraries for Natural Products Discovery Christopher J. Silva, Paul Brian, and Todd Peterson 13. Higher-Throughput Screening Assays With Human Hepatocytes for Hepatotoxicity, Metabolic Stability, and Drug-Drug Interaction Potential Albert P. Li 14. High-Throughput Screening for Metabolism-Based Drug-Drug Interactions Vaughn P. Miller and Charles L. Crespi 15. The ATCG of Drug Discovery Prabhavathi B. Fernandes 16. Genomics/Functional Proteomics for Ilentification of New Targets A. Donny Strosberg 17. Bioinformatics: Identification of Novel Targets and Their Characterization Chandra S. Ramanathan 18. The Evolution of Laboratory Automation Jack Elands 19. Robotics and Automation Robert F. Trinka and Franz E. Leichtfried 20. Assay Miniaturization: Developing Technologies and Assay Formats Kevin R. Oldenburg, Ilona Kariv, Ji-hu Zhang, Thomas D. Y. Chung, and Siqi Lin 21. Screening in the NanoWorld: Single-Molecule Spectroscopy and Miniaturized High-Throughput Screening Rodney Turner, Dirk Ullmann, and Sylvia Sterrer Index. A presentation of screening techniques, modern technologies, and high-capacity instrumentation for increased productivity in the development and discovery of new drugs, chemical compounds, and targeted delivery of pharmaceuticals. It contains practical applications and examples of strategies in cell-based and cell-free screens as well as homogeneous, fluorescence, chemiluminescence, and radioactive-based technologies.