Handbook of Cell-Penetrating Peptides, Second Edition (Pharmacology and Toxicology: Basic and Clinical Aspects)

Since the first Handbook of Cell-Penetrating Peptides was prepared in 2001, the wealth of new information on the use of these peptides as transport systems has in fact served to confound the field. The constant internal change in the field of cell-penetrating peptides (CPPs) is due to recent research uncovering apparent ambiguities in cellular uptake. There is still neither a common terminology nor a uniform explanation for the penetrative mechanism of cell-penetrating peptides. In this second edition of the Handbook of Cell-Penetrating Peptides, the authors summarize the current state of the field including recent reevaluations of earlier studies of CPP mechanisms. Beginning with an overview of the classes of peptides and their individual uptake mechanisms, from the earlier lipid models to the more recent endocytotic pathways, the book demonstrates the diversity and the opportunity for these biologically active proteins to serve as future drug leads. The text then covers the use of CPPs in gene modulation, addressing the application of antisense and decoy oligonucleotides, as well as the new avenue of research targeting specific tumors and other tissues-questions that had barely been asked when the first edition was published. By summarizing the diffuse information regarding CPPs, including the ambiguities and variety of mechanisms, the Handbook of Cell-Penetrating Peptides provides the most solid foundation available from which to expand the potential of this rapidly growing field of medicine. Doody Review Services Reviewer: Grace L Guo, PhD(University of Kansas Medical Center) Description: This book provides a comprehensive review of cell-penetrating peptides (CPP). It covers the introduction of CPP, the mechanism by which CPP functions, and application of CPP in biological pathway discovery and disease treatment, e.g. tumor therapy. This review will be of great interest to a broad range of scientific readers, including cell biologists and cancer researchers. Purpose: The purpose is to provide an update in the areas that invoke the most interest in cell-penetrating peptide research. Audience: The authors target general scientific researchers at both the basic and clinical science level. The authors are leading authorities in the field, which contributes to the success of this excellent review series. Features: This review covers various aspects of cell-penetrating peptide research, with the focus on CPP introduction and application of CPP in signal transduction and tumor treatment. It provides the up-to-date coverage on the use of CPP in biological research and cancer therapy. Assessment: This book is very informative for both basic science researchers and physicians, providing an update in many areas fundamental to the research of CPP. It provides an authoritative review on an exciting research topic and is an excellent reference on recent progress in CPP research. Preface......Page 6 Editor......Page 10 Contributors......Page 12 Contents......Page 18 Part I Classes and Prediction of Cell-Penetrating Peptides......Page 24 Penetratins......Page 28 Tat-Derived Cell-Penetrating Peptides: Discovery, Mechanism of Cell Uptake, and Applications to the Delivery of Oligonucleotides......Page 52 Model Amphipathic Peptides......Page 66 Cell-Penetrating cis-g-Amino-L-Proline-Derived Peptides......Page 84 Prediction of Cell-Penetrating Peptides......Page 100 Part II Mechanisms of Cell-Penetrating Peptides......Page 110 Inverted Lipid Models: A Pathway for Peptide Internalization?......Page 112 Membrane Interactions of Cell-Penetrating Peptides......Page 132 Interactions of Cell-Penetrating Peptides with Model Membranes......Page 162 The Import Mechanism of Cationic Cell-Penetrating Peptides and Its Implications for the Delivery of Peptide Inhibitors of Signal......Page 184 Mechanisms of Cargo Delivery by Transportans......Page 206 Tat-Mediated Peptide/Protein Transduction In Vivo......Page 224 Proteoglycans as Endocytosis Receptors for CPPs......Page 242 Part III Functionality of Shuttling Proteins and Protein-Derived Peptides......Page 258 Cell Permeable Peptides and Messenger Proteins, from a Serendipitous Observation to a New Signaling Mechanism......Page 262 Pharmacology and In Vivo Efficay of Pepducins in Hemostasis and Arterial Thrombosis......Page 268 HOXB4 Homeoprotein Transfer Promotes the Expansion of Hematopoietic Stem Cells......Page 282 Applications of Cell-Penetrating Peptides as Signal Transduction Modulators......Page 296 SynB Vectors as Drug Carriers......Page 316 Part IV Applications of Cell-Penetrating Peptides in Gene Modulation and Protein Transport......Page 332 Peptide Conjugates of Oligonucleotide Analogs and siRNA for Gene Expression Modulation......Page 336 Lego-Like Strategy for Assembling Multivalent Peptide-Based Vehicles Able to Deliver Molecular Cargoes into Cells......Page 352 Mechanistic Insights into Guanidinium-Rich Transporters Entering Cells......Page 376 Cell-Penetrating Short Interfering RNAs and Decoy Oligonucleotides......Page 398 A Noncovalent Peptide-Based Strategy for Peptide and Short Interfering RNA Delivery......Page 410 Part V Selective Targeting to Tumors and Other Tissues with Cell-Penetrating Peptides: In Vivo Applications......Page 432 Selective Delivery to Vascular Addresses: In Vivo Applications of Cell-Type-Specifi Cell-Penetrating Peptides......Page 436 In Vivo Applications of Cell-Penetrating Peptides......Page 446 Intracellular Delivery of Nanoparticles with CPPs......Page 462 Strategies to Incorporate CPPs into Synthetic Viruses for Tumor-Targeted Gene Therapy......Page 478 Cell-Permeable PentaPeptides Derived from Bax-Inhibiting Peptide......Page 492 Cellular Uptake of Liposomal and Micellar Carriers Mediated by ApoE-Derived Peptides......Page 506 Imaging the Influx of Cell-Penetrating Peptides into the Cytosol of Individual Live Cells......Page 528 Microbial MembranePenetrating Peptides and Their Applications......Page 538 Part VI CPP Methods......Page 558 Design and Synthesis of Cell-Penetrating Peptides......Page 560 Toxicity Methods for Cell-Penetrating Peptides......Page 576 Methods to Study the Translocation of Cell-Penetrating Peptides......Page 590 Index......Page 612 Since the first Handbook of Cell-Penetrating Peptides was prepared in 2001, the wealth of new information on the use of these peptides as transport systems has in fact served to confound the field. The constant internal change in the field of cell-penetrating peptides (CPPs) is due to recent research uncovering apparent ambiguities in cellular uptake. There is still neither a common terminology nor a uniform explanation for the penetrative mechanism of cell-penetrating peptides. In this second edition of the Handbook of Cell-Penetrating Peptides, the authors summarize the current state of the field including recent reevaluations of earlier studies of CPP mechanisms. Beginning with an overview of the classes of peptides and their individual uptake mechanisms, from the earlier lipid models to the more recent endocytotic pathways, the book demonstrates the diversity and the opportunity for these biologically active proteins to serve as future drug leads. The text then covers the use of CPPs in gene modulation, addressing the application of antisense and decoy oligonucleotides, as well as the new avenue of research targeting specific tumors and other tissues-questions that had barely been asked when the first edition was published. By summarizing the diffuse information regarding CPPs, including the ambiguities and variety of mechanisms, the Handbook of Cell-Penetrating Peptides provides the most solid foundation available from which to expand the potential of this rapidly growing field of medicine. The constant internal change in the field of cell-penetrating peptides (CPPs) is due to recent research uncovering apparent ambiguities in cellular uptake. There is still neither a common terminology nor a uniform explanation for the penetrative mechanism of cell-penetrating peptides. The authors summarize the current state of the field including recent reevaluations of earlier studies of CPP mechanisms. Beginning with an overview of the classes of peptides and their individual uptake mechanisms, from the earlier lipid models to the more recent endocytotic pathways, the book demonstrates the diversity and the opportunity for these biologically active proteins to serve as future drug leads. The text then covers the use of CPPs in gene modulation, addressing the application of antisense and decoy oligonucleotides, as well as the new avenue of research targeting specific tumors and other tissues-questions that had barely been asked when the first edition was published. By summarizing the diffuse information regarding CPPs, including the ambiguities and variety of mechanisms, this book provides the most solid foundation available from which to expand the potential of this rapidly growing field of medicine