راهنمای ژنتیک مخمر و زیست‌شناسی مولکولی، جلد ۱۹۴: راهنمای ژنتیک مخمر و زیست‌شناسی مولکولی (روش‌ها در آنزیم‌شناسی)

**Guide to Yeast Genetics and Molecular Biology** presents, for the first time, a comprehensive compilation of the protocols and procedures that have made __Saccharomyces cerevisiae__ such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study ''Your Favorite Gene'' in yeast. Key Features\* Basic Methods in Yeast Genetics\* Physical and genetic mapping\* Making and recovering mutants\* Cloning and Recombinant DNA Methods\* High-efficiency transformation\* Preparation of yeast artificial chromosome vectors\* Basic Methods of Cell Biology\* Immunomicroscopy\* Protein targeting assays\* Biochemistry of Gene Expression\* Vectors for regulated expression\* Isolation of labeled and unlabeled DNA, RNA, and protein Content: Contributors to volume 194 Pages xi-xvi Preface Page xvii Christine Guthrie, Gerald R. Fink Volumes in series Pages xix-xxxiv Process guide Pages xxxv-xxxvii [1] Getting started with yeast Original Research Article Pages 3-21 Fred Sherman [2] Micromanipulation and dissection of asci Original Research Article Pages 21-37 Fred Sherman, James Hicks [3] Mapping yeast genes Original Research Article Pages 38-57 Fred Sherman, Paul Wakem [4] Positional mapping of genes by chromosome blotting and chromosome fragmentation Original Research Article Pages 57-77 Sandra L. Gerring, Carla Connelly, Philip Hieter [5] Assay of yeast mating reaction Original Research Article Pages 77-93 George F. Sprague Jr. [6] Monitoring meiosis and sporulation in Saccharomyces cerevisiae Original Research Article Pages 94-110 Yona Kassir, Giora Simchen [7] Identifying sporulation genes, visualizing synaptonemal complexes, and large-scale spore and spore wall purification Original Research Article Pages 110-131 Rochelle Easton Esposito, Michael Dresser, Michael Breitenbach [8] Putting the HO gene to work: Practical uses for mating-type switching Original Research Article Pages 132-146 Ira Herskowitz, Robert E. Jensen [9] Spore enrichment Original Research Article Pages 146-149 Beth Rockmill, Eric J. Lambie, G.Shirleen Roeder [10] Analysis and manipulation of yeast mitochondrial genes Original Research Article Pages 149-165 Thomas D. Fox, Linda S. Folley, Julio J. Mulero, Thomas W. McMullin, Peter E. Thorsness, Lars O. Hedin, Maria C. Costanzo [11] DNA of Saccharomyces cerevisiae Original Research Article Pages 169-182 Peter Philippsen, Agathe Stotz, Christine Scherf [12] High-efficiency transformation of yeast by electroporation Original Research Article Pages 182-187 Daniel M. Becker, Leonard Guarente [13] Transmission of plasmid DNA to yeast by conjugation with bacteria Original Research Article Pages 187-195 Jack A. Heinemann, George F. Sprague Jr. [14] Cloning genes by complementation in yeast Original Research Article Pages 195-230 Mark D. Rose, James R. Broach [15] Gene isolation with λgt11 system Original Research Article Pages 230-238 Richard A. Young, Ronald W. Davis [16] Gene overexpression in studies of Saccharomyces cerevisiae Original Research Article Pages 239-251 Jasper Rine [17] Preparation of clone libraries in yeast artificial-chromosome vectors Original Research Article Pages 251-270 David T. Burke, Maynard V. Olson [18] Classical mutagenesis techniques Original Research Article Pages 273-281 Christopher W. Lawrence [19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast Original Research Article Pages 281-301 Rodney Rothstein [20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast Original Research Article Pages 302-318 Robert S. Sikorski, Jef D. Boeke [21] Recovery of plasmids from yeast into Escherichia coli : Shuttle vectors Original Research Article Pages 319-329 Jeffrey N. Strathern, David R. Higgins [22] Shuttle mutagenesis: Bacterial transposons for genetic manipulations in yeast Original Research Article Pages 329-342 Merl F. Hoekstra, H.Steven Seifert, Jac Nickoloff, Fred Heffron [23] Ty mutagenesis in Saccharomyces cerevisiae Original Research Article Pages 342-361 David J. Garfinkel, Jeffrey N. Strathern [24] Transformation of yeast directly with synthetic oligonucleotides Original Research Article Pages 362-369 Richard P. Moerschell, Goutam Das, Fred Sherman [25] Vectors for expression of cloned genes in yeast: Regulation, overproduction, and underproduction Original Research Article Pages 373-388 Jane C. Schneider, Leonard Guarente [26] Vectors for constitutive and inducible gene expression in yeast Original Research Article Pages 389-398 Mark Schena, Didier Picard, Keith R. Yamamoto [27] Preparation of high molecular weight RNA Original Research Article Pages 398-405 Karl Köhrer, Horst Domdey [28] Preparation and analysis of low molecular weight RNAs and small ribonucleoproteins Original Research Article Pages 405-415 Jo Ann Wise [29] Measurement of mRNA decay rates in Saccharomyces cerevisiae Original Research Article Pages 415-423 Roy Parker, David Herrick, Stuart W. Peltz, Allan Jacobson [30] Labeling of RNA and phosphoproteins in Saccharomyces cerevisiae Original Research Article Pages 423-428 Jonathan R. Warner [31] Tackling the protease problem in Saccharomyces cerevisiae Original Research Article Pages 428-453 Elizabeth W. Jones [32] Structural and functional analysis of yeast ribosomal proteins Original Research Article Pages 453-477 H.A. Raué, W.H. Mager, R.J. Planta [33] High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors Original Research Article Pages 477-490 T.J. Koerner, John E. Hill, Alan M. Myers, Alexander Tzagoloff [34] Production of proteins by secretion from yeast Original Research Article Pages 491-507 Donald T. Moir, Lance S. Davidow [35] Epitope tagging and protein surveillance Original Research Article Pages 508-519 Peter A. Kolodziej, Richard A. Young [36] Reverse biochemistry: Methods and applications for synthesizing yeast proteins in vitro Original Research Article Pages 520-535 Kevin Struhl [37] In vitro protein synthesis Original Research Article Pages 536-545 Michael J. Leibowitz, Francis P. Barbone, Denise E. Georgopoulos [38] RNA polymerase II transcription in vitro Original Research Article Pages 545-550 Neal F. Lue, Peter M. Flanagan, Raymond J. Kelleher III, Aled M. Edwards, Roger D. Kornberg [49] Direct sequence and footprint analysis of yeast DNA by primer extension Original Research Article Pages 550-562 Jon M. Huibregtse, David R. Engelke [40] Immunofluorescence methods for yeast Original Research Article Pages 565-602 John R. Pringle, Alison E.M. Adams, David G. Drubin, Brian K. Haarer [41] Preparation of yeast cells for thin-section electron microscopy Original Research Article Pages 602-608 Breck Byers, Loretta Goetsch [42] Immunogold labeling of yeast ultrathin sections Original Research Article Pages 608-626 Michael W. Clark [43] Analysis of mitochondrial function and assembly Original Research Article Pages 627-643 Michael P. Yaffe [44] Methods for studying the yeast vacuole Original Research Article Pages 644-661 Christopher J. Roberts, Christopher K. Raymond, Carl T. Yamashiro, Tom H. Stevens [45] Analysis of polypeptide transit through yeast secretory pathway Original Research Article Pages 662-674 Alex Franzusoff, Jonathan Rothblatt, Randy Schekman [46] In vitro protein translocation across microsomal membranes of Saccharomyces cerevisiae Original Research Article Pages 675-682 Pablo D. Garcia, William Hansen, Peter Walter [47] Analysis of glycoproteins from Saccharomyces cerevisiae Original Research Article Pages 682-697 P. Orlean, M.J. Kuranda, C.F. Albright [48] Yeast endocytosis assays Original Research Article Pages 697-710 Vjekoslav Dulic, Mark Egerton, Ibrahim Elguindi, Susan Raths, Birgit Singer, Howard Riezman [49] Inducing and assaying heat-shock response in Saccharomyces cerevisiae Original Research Article Pages 710-717 Charles M. Nicolet, Elizabeth A. Craig [50] Nucleolar-specific positive stains for optical and electron microscopy Original Research Article Pages 717-728 Michael W. Clark [51] Staining of actin with fluorochrome-conjugated phalloidin Original Research Article Pages 729-731 Alison E.M. Adams, John R. Pringle [52] Staining of bud scars and other cell wall chitin with Calcofluor Original Research Article Pages 732-735 John R. Pringle [53] Isolation of yeast nuclei Original Research Article Pages 735-749 John P. Aris, Günter Blobel [54] Analysis of chromosome segregation in Saccharomyces cerevisiae Original Research Article Pages 749-773 James H. Shero, Michael Koval, Forrest Spencer, Robert E. Palmer, Philip Hieter, Douglas Koshland [55] Genetic screens and selections for cell and nuclear fusion mutants Original Research Article Pages 774-792 Vivian Berlin, Julie A. Brill, Joshua Trueheart, Jef D. Boeke, Gerald R. Fink [56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe Original Research Article Pages 795-823 Sergio Moreno, Amar Klar, Paul Nurse [57] Genetic and physical maps of Saccharomyces cerevisiae Original Research Article Pages 827-863 Robert K. Mortimer, David Schild, C.Rebecca Contopoulou, Jonathan A. Kans Author index Pages 865-903 Subject index Pages 905-933