Glycosaminoglycans: Chemistry and Biology (Methods in Molecular Biology, 1229)

__Glycosaminoglycans: Chemistry and Biology__ emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches. Chapters focus on chemical and enzymatic synthesis of GAGs for therapeutic purposes; biophysical and biochemical methods for studying GAG-protein interactions; molecular approaches for modulating and defining GAG biosynthesis; informatics approaches for deciphering GAG code; computational approaches for establishing specific and/or non-specific interactions and genetic and biochemical tools for manipulating/visualizing glycosaminoglycan expression and studying their functions in a variety of model organisms. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, __Glycosaminoglycans: Chemistry and Biology__ serves as a valuable manual for cutting edge methodologies and practical tips to overcome any obstacles with experimentation pertaining to chemistry and biology of glycosaminoglycans. Automated Synthesis of Chondroitin Sulfate Oligosaccharides -- Chien-Fu Liang, Heung Sik Hahm, and Peter H. Seeberger Enzymatic Synthesis of Heparan Sulfate and Heparin -- April Joice [and six others] Production of Size-Defined Heparosan, Heparan Sulfate, and Heparin Oligosaccharides by Enzymatic Depolymerization -- Spencer Brown and Balagurunathan Kuberan Chemical Modification of Heparin and Heparosan -- Karthik Raman, Balagurunathan Kuberan, and Sailaja Arungundram Synthesis of Sulfur Isotope-Labeled Sulfate Donor, 3'-Phosphoadenosine-5'- Phosphosulfate, for Studying Glycosaminoglycan Functions -- Caitlin Mencio [and three others] Preparation of Isotope-Enriched Heparan Sulfate Precursors for Structural Biology Studies -- Xylophone V. Victor [and three others] Synthesis of Glycosaminoglycan Mimetics Through Sulfation of Polyphenols -- Rami A. Al-Horani [and three others] Synthesis of Selective Inhibitors of Heparan Sulfate and Chondroitin Sulfate Proteoglycan Biosynthesis -- Caitlin Mencio [and three others] Ascidian (Chordata-Tunicata) Glycosaminoglycans : Extraction, Purification, Biochemical, and Spectroscopic Analysis -- Mauro S.G. Pavão Human Blood Glycosaminoglycans : Isolation and Analysis -- Md. Ferdous Anower-E-Khuda and Koji Kimata Chromatographic Molecular Weight Measurements for Heparin, Its Fragments and Fractions, and Other Glycosaminoglycans -- Barbara Mulloy and John Hogwood Mass Spectrometric Methods for the Analysis of Heparin and Heparan Sulfate -- Miroslaw Lech, Ishan Capila, and Ganesh V. Kaundinya Validated Capillary Electrophoretic Assays for Disaccharide Composition Analysis of Galactosaminoglycans in Biologic Samples and Drugs/Nutraceuticals -- Athanasia P. Asimakopoulou [and five others] Fast Screening of Glycosaminoglycan Disaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) : Applications to Biologic Samples and Pharmaceutical Formulations -- Evgenia Karousou [and seven others] Capillary Electrophoretic Analysis of Isolated Sulfated Polysaccharides to Characterize Pharmaceutical Products -- Zachary Shriver and Ram Sasisekharan Methods for Measuring Exchangeable Protons in Glycosaminoglycans -- Consuelo N. Beecher and Cynthia K. Larive Heparan Sulfate Structure : Methods to Study N-Sulfation and NDST Action -- Anders Dagälv [and five others] Analysis of Hyaluronan Synthase Activity -- Davide Vigetti [and three others] A Rapid, Nonradioactive Assay for Measuring Heparan Sulfate C-5 Epimerase Activity Using Hydrogen/Deuterium Exchange-Mass Spectrometry -- Ponnusamy Babu [and three others] Aggrecan : Approaches to Study Biophysical and Biomechanical Properties -- Hadi Tavakoli Nia, Christine Ortiz, and Alan Grodzinsky Use of Flow Cytometry for Characterization and Fractionation of Cell Populations Based on Their Expression of Heparan Sulfate Epitopes -- Rebecca J. Holley [and five others] A Transgenic Approach to Live Imaging of Heparan Sulfate Modification Patterns -- Matthew Attreed and Hannes E. Bülow Informatics Tools to Advance the Biology of Glycosaminoglycans and Proteoglycans -- Lewis J. Frey Designing "High-Affinity, High-Specificity" Glycosaminoglycan Sequences Through Computerized Modeling -- Nehru Viji Sankaranarayanan [and three others] Using Isothermal Titration Calorimetry to Determine Thermodynamic Parameters of Protein-Glycosaminoglycan Interactions -- Amit K. Dutta, Jörg Rösgen, and Krishna Rajarathnam Characterizing Protein-Glycosaminoglycan Interactions Using Solution NMR Spectroscopy -- Prem Raj B. Joseph, Krishna Mohan Poluri, Krishna Mohan Sepuru, and Krishna Rajarathnam Glycosaminoglycan-Protein Interaction Studies Using Fluorescence Spectroscopy -- Rio S. Boothello, Rami A. Al-Horani, and Umesh R. Desai Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis -- Aiye Liang and Umesh R. Desai Histochemical Analysis of Heparan Sulfate 3-O-Sulfotransferase Expression in Mouse Brain -- Tomio Yabe and Nobuaki Maeda Keratan Sulfate : Biosynthesis, Structures, and Biological Functions -- Kenji Uchimura The Sulfs : Expression, Purification, and Substrate Specificity -- Kenji Uchimura The Detection of Glycosaminoglycans in Pancreatic Islets and Lymphoid Tissues -- Marika Bogdani [and five others] Nonradioactive Glycosyltransferase and Sulfotransferase Assay to Study Glycosaminoglycan Biosynthesis -- Cheryl M. Ethen [and five others] Mapping Proteoglycan Functions with Glycosidases -- Mauricio Cortes, Leslie K. Cortes, and Nancy B. Schwartz Cell Substrate Patterning with Glycosaminoglycans to Study Their Biological Roles in the Central Nervous System -- Tony W. Hsiao [and three others] Analyzing the Role of Heparan Sulfate Proteoglycans in Axon Guidance In Vivo in Zebrafish -- Fabienne E. Poulain Murine Models in the Evaluation of Heparan Sulfate-Based Anticoagulants -- David Gailani, Qiufang Cheng, and Ivan S. Ivanov Genetic Approaches in the Study of Heparan Sulfate Functions in Drosophila -- Masahiko Takemura and Hiroshi Nakato Measuring Sulfatase Expression and Invasion in Glioblastoma -- Anna Wade [and three others] Synthesis and Biomedical Applications of Xylosides -- Mausam Kalita [and four others] A Strategic Approach to Identification of Selective Inhibitors of Cancer Stem Cells -- Nirmita Patel, Somesh Baranwal, and Bhaumik B. Patel Analysis of the Heavy-Chain Modification and TSG-6 Activity in Pathological Hyaluronan Matrices -- Mark E. Lauer [and three others] Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration -- Hong Qiu [and three others] Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions -- James A. Vassie, John M. Whitelock, and Megan S. Lord Role of Glycosaminoglycans in Infectious Disease -- Akiko Jinno and Pyong Woo Park Isolation and Purification of Versican and Analysis of Versican Proteolysis -- Simon J. Foulcer, Anthony J. Day, and Suneel S. Apte Analysis of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility in Myofibroblast Differentiation -- Adam C. Midgley and Timothy Bowen. Front Matter....Pages i-xvi Front Matter....Pages 1-1 Front Matter....Pages 3-10 Front Matter....Pages 11-19 Front Matter....Pages 21-29 Back Matter....Pages 31-36 ....Pages 37-42