Genomics and Proteomics in Nutrition (Nutrition in Health and Disease)

With contributions from 66 world-renowned authorities on the subject, Genomics And Proteomics In Nutrition focuses on the effect of genetic expression on protein production, establishing links between gene expression and nutrition, the influence of micro- and macronutrients on cellular homeostasis, and the relationship between diet, genetic predisposition, and human disease. Shows how bioinformatics and functional genomic databases can transform molecular biology research using a yeast ELO2/ human HELO1 protein cloning example. A cutting-edge reference, Genomics and Proteomics in Nutrition reveals new applications and insights in nutrition science and explores tools for nutrition research in the post-genomic era. Part I: Mouse models of human diseases and gene transcription. 1. Genetic modifiers in rodent models of obesity / Yun Wang, Patsy M. Nishina, and Jürgen K. Naggert 2. Quantitative trait loci in obesity and type II diabetes in rodents / Jung Han Kim 3. The human sweet tooth and its relationship to obesity / Amanda H. McDaniel and Danielle R. Reed 4. The adipose renin-angiotensin system: genetics, regulation, and physiologic function / Brynn H. Voy ... [et al.] 5. Regulation of fat synthesis and adipogenesis / Kee-Hong Kim ... [et al.] 6. Transcriptional regulation of energy metabolism in liver / Manabu T. Nakamura. Part II: Genomics: large-scale analysis of gene expression by microarray. 7. Design and analysis of microarray studies for obesity research / Marie-Pierre St.-Onge ... [et al.] 8. Solving clinical problems in nutrition research with microarrays / Steven R. Smith ... [et al.] 9. Genomic approaches to understanding vitamin D action / James C. Fleet 10. Gene expression profiling in adipose tissue / Naima Moustaid-Moussa ... [et al.] 11. Phytochemicals and gene expression / Orsolya Mezei and Neil F. Shay 12. Gene expression profiling of immune cells: application for understanding aging of immune cells / Sung Nim Han and Simin Nikbin Meydani 13. Improving the nutritional value of cereal grains using a genomic approach / Liang Shi and Tong Zhu. Part III: Proteomics: large-scale analysis of protein expression and regulation. 14. Proteomics and genomics to detail responses to extracellular stimuli / James A. Carroll and Clayton E. Mathews 15. 2D gel proteomics studies on leukocytes / Ivan C. Gerling and Michael J. Pabst 16. Proteomics: tools for nutrition research in the postgenomic era / Arthur Grider 17. Proteomic analysis of the adipocyte secretome / Nieves Ibarrola, Akhilesh Pandey, and Chaerkady Raghothama 18. Mass spectrometry strategies for proteomic studies / Maya Belghazi and Hélène Rogniaux Bioinformatics tools / Simon M. Lin, Kimberly F. Johnson, and Seth W. Kullman 20. Statistical principles for analysis of array experiments / Arnold M. Saxton and E. Barry Moser. Book Cover......Page 1 Half-title......Page 2 Title......Page 4 Copyright......Page 5 Preface......Page 6 Contents......Page 8 Contributors......Page 12 1. Genetic Modifiers in Rodent Models of Obesity......Page 18 2. Quantitative Trait Loci for Obesity and Type II Diabetes in Rodents......Page 31 3. The Human Sweet Tooth and Its Relationship to Obesity......Page 61 4. The Adipose Renin-Angiotensin System: Genetics, Regulation, and Physiologic Function......Page 77 5. Regulation of Fat Synthesis and Adipogenesis......Page 94 6. Transcriptional Regulation of Energy Metabolism in Liver......Page 124 7. Design and Analysis of Microarray Studies for Obesity Research......Page 147 8. Solving Clinical Problems in Nutrition Research with Microarrays......Page 198 9. Genomic Approaches to Understanding Vitamin D Action......Page 226 10. Gene Expression Profiling in Adipose Tissue......Page 245 11. Phytochemicals and Gene Expression......Page 265 12. Gene Expression Profiling of Immune Cells: Application for Understanding Aging of Immune Cells......Page 279 13. Improving the Nutritional Value of Cereal Grains Using a Genomic Approach......Page 296 14. Proteomics and Genomics to Detail Responses to Extracellular Stimuli......Page 309 15. 2-D Gel Proteomics Studies on Leukocytes......Page 331 16. Proteomics: Tools for Nutrition Research in the Postgenomic Era......Page 349 17. Proteomic Analysis of the Adipocyte Secretome......Page 368 18. Mass Spectrometry Strategies for Proteomic Studies......Page 386 19. Bioinformatics Tools......Page 416 20. Statistical Principles for Analysis of Array Experiments......Page 438 Index......Page 457 "Genomics and Proteomics in Nutrition investigates the latest proteomic studies for clear understanding of the role of nutrients and drugs in nutrient and cellular metabolism ... explores advances in our understanding of obesity, birth defects, and cancer ... presents selected examples showcasing the effect of phytochemicals on gene expression in different model systems ... strives to identify molecular markers associated with various disease states for improved diagnostic and treatment regimens ... and familiarizes the clinical investigator with day-to-day problems encountered in the design, implementation, and evaluation of microarray analysis of clinical samples and provides the background necessary to develop solutions to these problems."--Jacket The identification of single gene mutations leading to obesity in the mouse has greatly enhanced our understanding of the regulation of bodyweight and energy metabolism.