فناوری و الگوریتم‌های توالی‌یابی ژنوم

The 2003 completion of the Human Genome Project was just one step in the evolution of DNA sequencing. Now from a "who's who" of pioneers in the field comes the latest genome sequencing and assembly advances that are redefining the field. This trail-blazing book gives researchers, unparalleled access to state-of-the-art DNA sequencing technologies, new algorithmic sequence assembly techniques, and emerging methods for both resequencing and genome analysis that together form the most solid foundation possible for tackling experimental and computational challenges in the genome sciences today. Including critiques of existing techniques, this far-reaching resource offers researchers assistance in achieving more rapid and accurate DNA sequencing and developing the next generation of high-throughput methods and devices. From a “who’s who” of pioneers in the field comes a unique resource covering the latest advances in next-generation genome sequencing and assembly. This groundbreaking book includes non-conventional techniques that are paving the way to potential new biomedical applications. You get unparalleled access to state-of-the-art DNA sequencing technologies, new algorithmic sequence assembly techniques, and emerging methods for both resequencing and de novo genome analysis which all together give you the most solid foundation possible for tackling the full range of experimental and computational challenges in the genome sciences today. This first-of-its-kind resource walks you step-by-step through new sequencing technologies involving sequencing by synthesis, nanopore sequencing, polymerase colonies, and single molecule sequencing. It then explores the latest algorithmic techniques for DNA sequence assembly and offers expert insight into assembly packages like Arachne, EULER, CAP, AMASS, and Celera. You also get coverage of emerging biotechnology-oriented approaches to the resequencing and analysis of genomes, with the latest techniques for SNP, genome rearrangement, and comparative sequencing and assembly. Moreover, the book critiques strengths and weaknesses of existing techniques and spotlights possible ways to improve them. The most comprehensive treatment of the field’s frontier topics, this definitive volume will prove indispensable in your efforts to achieve more rapid and accurate DNA sequencing and to develop the next generation of high-throughput methods and devices. An overview of new DNA sequencing technology -- Array-based pyrosequencing technology -- The role of resequencing arrays in revolutionizing DNA sequencing -- Polony sequencing -- Genome sequencing: a complex path to personalized medicine -- Overview of genome assembly techniques -- Fragment assembly algorithms -- Assembly for double-ended short read sequencing technologies -- Genome characterization in the post-human genome project era -- The haplotyping problem: an overview of computational models and solutions -- Analysis of genomic alterations in cancer -- High-throughput assessments of epigenomics in human disease -- Comparative sequencing, assembly, and anchoring "From a who's who of pioneers in the field comes a unique resource covering the latest advances in next generation genome sequencing and assembly. This groundbreaking book includes non-conventional techniques that are paving the way to potential new biomedical applications. Practitioners find unparalleled access to state-of-the-art DNA sequencing technologies, new algorithmic sequence assembly techniques, and emerging methods for both resequencing and de novo genome analysis, which all together offer the most solid foundation possible for tackling the full range of experimental and computational challenges in the genome sciences today."--Jacket From a "who's who" of pioneers in the field, this unique resource offers unparalleled access to state-of-the-art DNA sequencing technologies, new algorithmic sequence assembly techniques, and emerging methods for both resequencing and de novo genome analysis. The book offers a solid foundation for tackling the full range of experimental and computational challenges in the genome sciences today