Genetically engineered toxins

Cover......Page 1 Half Title......Page 2 Series Page......Page 3 Title Page......Page 4 Copyright Page......Page 5 Dedication......Page 6 Introduction to the Series......Page 7 Preface......Page 8 Table of Contents......Page 12 Contributors......Page 15 I: Strategies and Techniques......Page 20 1. General Methods......Page 22 2. Cloning Strategies......Page 62 3. Protein Engineering Strategies......Page 74 4. General Strategies in In Vivo Animal Modeling......Page 82 II: Ricin......Page 98 5. Molecular Cloning of Ricin......Page 100 6. Expression of Plant-Derived Ribosome-Inactivating Proteins in Heterologous Systems......Page 118 7. The Structure of Plant Toxins as a Guide to Rational Design......Page 152 8. Chemical and Genetic Characterization of the Enzymatic Activity Associated with Ricin A Chain......Page 170 9. Chimeric Proteins Containing Ricin A Chain......Page 202 III: Plant Hemitoxins......Page 210 10. Studies on Ribosome-Inactivating Proteins from Saponaria officinalis......Page 212 11. Cloning and Expression of Trichosanthin and a-Momorcharin cDNA......Page 232 12. Cloning and Expression of a Luffa Ribosome-Inactivating Protein-Related Protein......Page 242 IV: Fungal Ribotoxins......Page 254 13. The Aspergillus Ribonucleolytic Toxins (Ribotoxins)......Page 256 14. An Efficient Expression System for a-Sarcin in Escherichia coli......Page 278 V: Diphtheria Toxin......Page 290 15. Diphtheria Toxin Cloning and Expression in Corynebacterium diphtheriae......Page 292 16. Diphtheria Toxin Expression in Escherichia coli......Page 326 17. The Structure of Diphtheria Toxin as a Guide to Rational Design......Page 358 18. Protein Engineering of Diphtheria Toxin: Development of Receptor-Specific Cytotoxic Agents for the Treatment of Human Disease......Page 384 19. In Vivo Studies with Chimeric Toxins: Interleukin-2 Fusion Toxins as Immunosuppressive Agents......Page 402 20. Initial Clinical Experiences with an lnterleukin-2 Fusion Toxin (DAB-IL-2)......Page 414 VI: Pseudomonas Exotoxins......Page 422 21. Pseudomonas aeruginosa Exotoxin A......Page 424 22. Expression of Growth Factor-Toxin Fusion Proteins......Page 444 23. The Structure of Pseudomonas Exotoxin A as a Guide to Rational Design......Page 458 24. Generation of Chimeric Toxins......Page 466 VII: Conclusions......Page 482 25. Genetically Engineered Toxins in Perspective......Page 484 Appendix: Primary Amino Acid Sequences of Toxins......Page 498 Index......Page 504 Presenting all preclinical and clinical information available on genetically engineered toxins, this unique, single-source reference provides the most up-to-date methods and practical examples for conducting clinical studies in toxin molecular biology.;Reviewing difficult problems and their solutions, Genetically Engineered Toxins discusses techniques for clo;ning, expressing, and purifying recombinant toxins and genetically modified recombinant toxins; documents structure-function relationships in toxins, including comparative information; supplies theory and illustrations of chimeric toxins; delineates the preclinical assessments of new reagents; and summarizes approaches to drug design.;With over 1100 literature citations, Genetically Engineered Toxins is an invaluable resource for biochemists, molecular biologists, biotechnologists, pharmacologists, toxicologists, X-ray crystallographers, enzymologists, oncologists, hematologists, immunologists, rheumatologists, botanists, and graduate-level students in molecular biology, biotechnology, and clinical oncology courses