Genetic Transformation Systems in Fungi, Volume 1 (Fungal Biology)

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. ​ Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (__Phytophthora__ and __Botrytis__) and mammalian infection (__Penicillium marneffei__ and __Candida__). __Genetic Transformation Systems in Fungi, Volume 1__ provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits. Front Matter....Pages i-xv Front Matter....Pages 1-1 Fungal Transformation: From Protoplasts to Targeted Recombination Systems....Pages 3-18 Front Matter....Pages 19-19 Protoplast Transformation for Genome Manipulation in Fungi....Pages 21-40 Trichoderma Transformation Methods....Pages 41-48 Transformation of Mucor circinelloides f. lusitanicus Protoplasts....Pages 49-59 Transformation of Saccharomyces cerevisiae : Spheroplast Method....Pages 61-63 Front Matter....Pages 65-65 Electroporation Mediated DNA Transformation of Filamentous Fungi....Pages 67-79 Chemical Transformation of Candida albicans ....Pages 81-85 Electroporation of Pichia pastoris ....Pages 87-91 Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareri) ....Pages 93-97 Front Matter....Pages 99-99 Biolistic Transformation for Delivering DNA into the Mitochondria....Pages 101-117 Biolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants....Pages 119-127 Use of the Biolistic Particle Delivery System to Transform Fungal Genomes....Pages 129-133 Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment....Pages 135-140 Front Matter....Pages 141-141 Agrobacterium tumefaciens- Mediated Transformation....Pages 143-162 Agrobacterium tumefaciens- Mediated Transformation of Pucciniomycotina Red Yeasts....Pages 163-168 Glass-Bead and Agrobacterium-Mediated Genetic Transformation of Fusarium oxysporum ....Pages 169-174 Front Matter....Pages 175-175 High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method....Pages 177-186 Transformation of Intact Cells of Saccharomyces cerevisiae : Lithium Methods and Possible Underlying Mechanism....Pages 187-192 Transformation of Lithium Acetate-treated Neurospora crassa ....Pages 193-197 Front Matter....Pages 199-199 Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells....Pages 201-207 Front Matter....Pages 199-199 Transformation of Fungi Using Shock Waves....Pages 209-219 Front Matter....Pages 221-221 Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics....Pages 223-237 Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle....Pages 239-252 Front Matter....Pages 253-253 Recombination and Gene Targeting in Neurospora ....Pages 255-262 Efficient Generation of Aspergillus niger Knock Out Strains by Combining NHEJ Mutants and a Split Marker Approach....Pages 263-272 REMI in Molecular Fungal Biology....Pages 273-287 TALEN-Based Genome Editing in Yeast....Pages 289-307 Back Matter....Pages 309-318